Mouse IL-33 ELISA Kit
$245.00 – $350.00
| Related Target | |
|---|---|
| Species | mouse |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | Serum, plasma: 20 μL, cell culture supernatant: 100 μL |
| Sensitivity | 8.74 pg/mL |
| Array Range | 46.88 pg/mL – 3000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 85% – 117% |
| Average Recovery | 98% |
ELISA Kit Detail Information
| Related Target | |
|---|---|
| Species | mouse |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | Serum, plasma: 20 μL, cell culture supernatant: 100 μL |
| Sensitivity | 8.74 pg/mL |
| Array Range | 46.88 pg/mL – 3000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 85% – 117% |
| Average Recovery | 98% |
| Intra Precision | 4.9% – 7.2% |
| Inter Precision | 4.9% – 8.1% |
| Plate | Detachable 96-well plate |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-33 monoclonal antibody Mouse IL-33 freeze-dried standard IL-33 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IL-33 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-33 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-33 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Related Targets
IL33
IL33 Target Infomation Overview
- Target Symbol: IL33, interleukin 33
- Gene Groups: Interleukins
- Alias: DVS27; DKFZp586H0523; NF-HEV; IL1F11
- Previous Names: C9orf26
- Alias Names: DVS27-related protein; nuclear factor for high endothelial venules; interleukin-1 family, member 11; chromosome 9 open reading frame 26 (NF-HEV)
IL33, interleukin 33 Target Infomation by Species
- Human
- Mouse
- Rat
Human IL33 Target Information
- Target Symbol: IL33, interleukin 33
- Alias:
- C9orf26
- DKFZp586H0523
- DVS27
- DVS27-related protein
- IL-1F11
- IL-33
- IL1F11
- interleukin-1 family member 11
- interleukin-33
- NF-HEV
- NFEHEV
- nuclear factor for high endothelial venules
- nuclear factor from high endothelial venules
- RP11-575C20.2
- NCBI_Gene: 90865
- UniProtKB: O95760
Human IL33 Predicted Functions
Enables cytokine activity. Involved in negative regulation of transcription by RNA polymerase II; positive regulation of chemokine production; and positive regulation of macrophage activation. Located in nucleoplasm. Part of cytoplasm and nucleus. Implicated in candidiasis; inflammatory bowel disease; and peptic ulcer disease. Biomarker of several diseases, including colitis (multiple); common cold; gout; liver disease (multiple); and tuberculosis (multiple).
Mouse Il33 Target Information
- Target Symbol: Il33, interleukin 33
- Alias:
- 9230117N10Rik
- Il-33
- Il1f11
- NF-HEV
- RIKEN cDNA 9230117N10 gene
- NCBI_Gene: 77125
Mouse Il33 Predicted Functions
Enables cytokine activity and interleukin-33 receptor binding activity. Involved in several processes, including interleukin-33-mediated signaling pathway; microglial cell activation involved in immune response; and regulation of gene expression. Acts upstream of or within several processes, including negative regulation of macrophage proliferation; positive regulation of cellular defense response; and positive regulation of macromolecule metabolic process. Located in nucleus. Is expressed in several structures, including adrenal gland; alimentary system; genitourinary system; nervous system; and sensory organ. Used to study Alzheimer’s disease. Human ortholog(s) of this gene implicated in candidiasis; inflammatory bowel disease; and peptic ulcer disease. Orthologous to human IL33 (interleukin 33).
Rat Il33 Target Information
- Target Symbol: Il33, interleukin 33
- Alias:
- IL-33
- interleukin-33
- LOC361749
- RGD1311155
- similar to RIKEN cDNA 9230117N10
- NCBI_Gene: 361749
Rat Il33 Predicted Functions
Predicted to enable cytokine activity and interleukin-33 receptor binding activity. Involved in several processes, including cellular response to mechanical stimulus; positive regulation of oligodendrocyte differentiation; and regulation of sensory perception of pain. Located in nucleus. Biomarker of acute myocardial infarction; colon cancer; and diabetes mellitus. Human ortholog(s) of this gene implicated in candidiasis; inflammatory bowel disease; and peptic ulcer disease. Orthologous to human IL33 (interleukin 33).
Products with the Same Target - IL33
Citations for Mouse IL-33 ELISA Kit
There are no citations for this product yet.
If you buy this product and mention it in your thesis, we welcome you to provide us with the thesis information. As a thank you, we will give you some gifts, such as discount coupons.
Citations data is updating...
Competitive ELISA: Principles, Methods, and Key Differences
Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.
ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol
Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.
ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects
Learn whether to use serum or plasma for ELISA testing, including preparation protocols, key differences (fibrinogen, clotting factors), and how hemolysis impacts results. Essential guide for accurate immunoassays.
Related products
-
-
- EK1202
- ELISA Kit
Human Angiotensinogen/AGT/SerpinA8 ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1193
- ELISA Kit
Human Apolipoprotein H/Apo H ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1223
- ELISA Kit
Human Apolipoprotein D/Apo D ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1120
- ELISA Kit
Human Activin A ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1122
- ELISA Kit
Human Angiopoietin-1/ANG-1 ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1272
- ELISA Kit
Human B7-1/CD80 ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
Reviews
There are no reviews yet.