Mouse IL-33 High Sensitivity ELISA Kit

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EK233HS

Mouse IL-33 High Sensitivity ELISA Kit

$266.00 – $380.00

Related Target
Speciesmouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 20 μL, cell culture supernatant: 100 μL
Sensitivity0.26 pg/mL
Array Range7.81 pg/mL – 500 pg/mL
Assay Time3.5 h
Recovery80% – 102%
Average Recovery87%
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SKU: EK233HS Category:

ELISA Kit Detail Information

Related Target
Speciesmouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 20 μL, cell culture supernatant: 100 μL
Sensitivity0.26 pg/mL
Array Range7.81 pg/mL – 500 pg/mL
Assay Time3.5 h
Recovery80% – 102%
Average Recovery87%
Intra Precision2.2% – 3.2%
Inter Precision2.6% – 3.5%
PlateDetachable 96-well plate
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-33 monoclonal antibody
Mouse IL-33 high sensitivity freeze-dried standard
IL-33 detect Antibody
Standard Diluent
HRP-labeled streptavidin
Signal enhancer concentrate
Signal enhancer diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IL-33 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IL-33 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-33 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

IL33

IL33 Target Infomation Overview

  • Target Symbol: IL33, interleukin 33
  • Gene Groups: Interleukins
  • Alias: DVS27; DKFZp586H0523; NF-HEV; IL1F11
  • Previous Names: C9orf26
  • Alias Names: DVS27-related protein; nuclear factor for high endothelial venules; interleukin-1 family, member 11; chromosome 9 open reading frame 26 (NF-HEV)

IL33, interleukin 33 Target Infomation by Species

  • human iconHuman
  • mouse iconMouse
  • rat iconRat

Human IL33 Target Information

  • Target Symbol: IL33, interleukin 33
  • Alias:
    • C9orf26
    • DKFZp586H0523
    • DVS27
    • DVS27-related protein
    • IL-1F11
    • IL-33
    • IL1F11
    • interleukin-1 family member 11
    • interleukin-33
    • NF-HEV
    • NFEHEV
    • nuclear factor for high endothelial venules
    • nuclear factor from high endothelial venules
    • RP11-575C20.2
  • NCBI_Gene: 90865
  • UniProtKB: O95760

Human IL33 Predicted Functions

Enables cytokine activity. Involved in negative regulation of transcription by RNA polymerase II; positive regulation of chemokine production; and positive regulation of macrophage activation. Located in nucleoplasm. Part of cytoplasm and nucleus. Implicated in candidiasis; inflammatory bowel disease; and peptic ulcer disease. Biomarker of several diseases, including colitis (multiple); common cold; gout; liver disease (multiple); and tuberculosis (multiple).

Mouse Il33 Target Information

Mouse Il33 Predicted Functions

Enables cytokine activity and interleukin-33 receptor binding activity. Involved in several processes, including interleukin-33-mediated signaling pathway; microglial cell activation involved in immune response; and regulation of gene expression. Acts upstream of or within several processes, including negative regulation of macrophage proliferation; positive regulation of cellular defense response; and positive regulation of macromolecule metabolic process. Located in nucleus. Is expressed in several structures, including adrenal gland; alimentary system; genitourinary system; nervous system; and sensory organ. Used to study Alzheimer’s disease. Human ortholog(s) of this gene implicated in candidiasis; inflammatory bowel disease; and peptic ulcer disease. Orthologous to human IL33 (interleukin 33).

Rat Il33 Target Information

Rat Il33 Predicted Functions

Predicted to enable cytokine activity and interleukin-33 receptor binding activity. Involved in several processes, including cellular response to mechanical stimulus; positive regulation of oligodendrocyte differentiation; and regulation of sensory perception of pain. Located in nucleus. Biomarker of acute myocardial infarction; colon cancer; and diabetes mellitus. Human ortholog(s) of this gene implicated in candidiasis; inflammatory bowel disease; and peptic ulcer disease. Orthologous to human IL33 (interleukin 33).

Products with the Same Target - IL33

Mouse IL-33 ELISA Kit

$245.00 – $350.00

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