Human GM-CSF ELISA Kit

$350.00$450.00

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TARGETSCSF2
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity4.76 pg/mL
Range1.56 pg/mL – 100 pg/mL
Assay Time3.5 h
Recovery97% – 110%
Average Recovery1.04
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity4.76 pg/mL
Range1.56 pg/mL – 100 pg/mL
Assay Time3.5 h
Recovery97% – 110%
Average Recovery1.04
Intra Precision2.5% – 2.9%
Inter-Precision3.7% – 4.9%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-GM-CSF monoclonal antibody
Human GM-CSF freeze-dried standard
GM-CSF detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human GM-CSF antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, GM-CSF present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of GM-CSF in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

CSF2

CSF2 Target Infomation Overview

  • Target Symbol: CSF2, colony stimulating factor 2
  • Alias: GM-CSF; GMCSF
  • Alias Names: sargramostim; molgramostim; granulocyte-macrophage colony stimulating factor; colony stimulating factor 2 (granulocyte-macrophage)

CSF2, colony stimulating factor 2 Target Infomation by Species

HumanMouseRat

Human CSF2 Target Information

  • Target Symbol: CSF2, colony stimulating factor 2
  • Alias:
    • colony stimulating factor 2 (granulocyte-macrophage)
    • colony-stimulating factor
    • CSF
    • GMCSF
    • granulocyte macrophage-colony stimulating factor
    • granulocyte-macrophage colony stimulating factor
    • granulocyte-macrophage colony-stimulating factor
    • MGC131935
    • MGC138897
    • molgramostim
    • molgramostin
    • sargramostim
  • NCBI_Gene: 1437
  • UniProtKB: P04141

Human CSF2 Predicted Functions

Enables cytokine activity. Involved in several processes, including cellular response to granulocyte macrophage colony-stimulating factor stimulus; myeloid leukocyte differentiation; and positive regulation of macromolecule metabolic process. Located in extracellular space and intracellular membrane-bounded organelle. Implicated in acute myeloid leukemia; juvenile myelomonocytic leukemia; neutropenia; pulmonary alveolar proteinosis; and visceral leishmaniasis. Biomarker of COVID-19; asthma; chronic obstructive pulmonary disease; and temporal arteritis.

Mouse Csf2 Target Information

Mouse Csf2 Predicted Functions

Enables cytokine activity. Acts upstream of or within several processes, including embryonic placenta development; myeloid leukocyte differentiation; and positive regulation of cell population proliferation. Located in extracellular space. Is expressed in several structures, including genitourinary system; hemolymphoid system; labyrinthine zone; liver; and lung. Used to study pulmonary alveolar proteinosis. Human ortholog(s) of this gene implicated in acute myeloid leukemia; juvenile myelomonocytic leukemia; neutropenia; pulmonary alveolar proteinosis; and visceral leishmaniasis. Orthologous to human CSF2 (colony stimulating factor 2).

Rat Csf2 Target Information

  • Target Symbol: Csf2, colony stimulating factor 2
  • Alias:
    • colony stimulating factor 2 (granulocyte-macrophage)
    • colony-stimulating factor
    • CSF
    • Gm-csf
    • Gmcsf
    • granulocyte-macrophage colony-stimulating factor
  • NCBI_Gene: 116630
  • UniProtKB: P48750

Rat Csf2 Predicted Functions

Enables cytokine activity. Involved in several processes, including cellular response to lipopolysaccharide; macrophage activation; and response to silicon dioxide. Located in extracellular space. Used to study Parkinsonism and myocardial infarction. Biomarker of abdominal aortic aneurysm; interstitial cystitis; and lung disease (multiple). Human ortholog(s) of this gene implicated in acute myeloid leukemia; juvenile myelomonocytic leukemia; neutropenia; pulmonary alveolar proteinosis; and visceral leishmaniasis. Orthologous to human CSF2 (colony stimulating factor 2).

CSF2 Target News

    8.185
    CSF2 upregulates CXCL3 expression in adipocytes to promote metastasis of breast cancer via the FAK signaling pathway
    Journal of Molecular Cell Biology
    Allergic Reaction
    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Lung Disease (Asthma & COPD)
    Multiple Sclerosis
    Osteoarthrosis & Rheumatoid Arthritis
    Sepsis
    Systemic Lupus Erythematosus
    Transplantation
    Tumor Marker

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