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Human PIGF High Sensitivity ELISA Kit
$380.00 – $480.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 10 μL |
| Sensitivity | 0.30 pg/mL |
| Range | 3.13 pg/mL – 200 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 82% – 104% |
| Average Recovery | 0.93 |
| Intra Precision | 1.6% – 3.1% |
| Inter-Precision | 2.6% – 3.4% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-PIGFA monoclonal antibody Human PIGFA freeze-dried standard PIGFA detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human PIGF antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the PIGF present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of PIGF in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
PIGF
PIGF Target Infomation Overview
- Target Symbol: PIGF, phosphatidylinositol glycan anchor biosynthesis class F
- Gene Groups: Phosphatidylinositol glycan anchor biosynthesis
- Alias Names: phosphatidylinositol glycan, class F
PIGF, phosphatidylinositol glycan anchor biosynthesis class F Target Infomation by Species
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Human PIGF Target Information
- Target Symbol: PIGF, phosphatidylinositol glycan anchor biosynthesis class F
- Alias:
- GPI11 homolog
- MGC32646
- MGC33136
- OORS
- phosphatidylinositol glycan anchor biosynthesis, class F
- phosphatidylinositol glycan, class f
- phosphatidylinositol-glycan biosynthesis class F protein
- PIG-F
- NCBI_Gene: 5281
- UniProtKB: Q07326
Human PIGF Predicted Functions
Predicted to enable mannose-ethanolamine phosphotransferase activity. Predicted to be involved in GPI anchor biosynthetic process. Predicted to be integral component of membrane. Predicted to be active in endoplasmic reticulum membrane.
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Mouse Pigf Target Information
- Target Symbol: Pigf, phosphatidylinositol glycan anchor biosynthesis, class F
- Alias:
- 无
- NCBI_Gene: 18701
Mouse Pigf Predicted Functions
Predicted to enable mannose-ethanolamine phosphotransferase activity. Involved in GPI anchor biosynthetic process. Located in endoplasmic reticulum membrane. Is expressed in several structures, including alimentary system; brain; respiratory system; sensory organ; and urinary system. Orthologous to human PIGF (phosphatidylinositol glycan anchor biosynthesis class F).
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Rat Pigf Target Information
- Target Symbol: Pigf, phosphatidylinositol glycan anchor biosynthesis, class F
- Alias:
- LOC681086
- phosphatidylinositol-glycan biosynthesis class F protein
- similar to Phosphatidylinositol-glycan biosynthesis, class F protein (PIG-F)
- NCBI_Gene: 681086
- UniProtKB: D3ZZU7
Rat Pigf Predicted Functions
Predicted to enable mannose-ethanolamine phosphotransferase activity. Predicted to be involved in GPI anchor biosynthetic process. Predicted to be integral component of membrane. Predicted to be active in endoplasmic reticulum membrane. Orthologous to human PIGF (phosphatidylinositol glycan anchor biosynthesis class F).
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PIGF Target News
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