Mouse IL-5 High Sensitivity ELISA Kit

$380.00$480.00

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TARGETSIL5
SPECIESMouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity0.14 pg/mL
Range1.56 pg/mL – 100 pg/mL
Assay Time3.5 h
Recovery104% – 107%
Average Recovery1.05
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity0.14 pg/mL
Range1.56 pg/mL – 100 pg/mL
Assay Time3.5 h
Recovery104% – 107%
Average Recovery1.05
Intra Precision4.8% – 5.0%
Inter-Precision3.0% – 5.3%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-5 monoclonal antibody
Mouse IL-5 freeze-dried standard
IL-5 detect Antibody
Standard Diluent
HRP-labeled streptavidin
Signal enhancer concentrate
Signal enhancer diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IL-5 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IL-5 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-5 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IL5

IL5 Target Infomation Overview

  • Target Symbol: IL5, interleukin 5
  • Gene Groups: Interleukins
  • Alias: IL-5; EDF; TRF
  • Alias Names: interleukin-5; T-cell replacing factor; B cell differentiation factor I; eosinophil differentiation factor; colony-stimulating factor, eosinophil; interleukin 5 (colony-stimulating factor, eosinophil)

IL5, interleukin 5 Target Infomation by Species

HumanMouseRat

Human IL5 Target Information

  • Target Symbol: IL5, interleukin 5
  • Alias:
    • B cell differentiation factor I
    • B-cell differentiation factor I
    • colony-stimulating factor, eosinophil
    • EDF
    • eosinophil differentiation factor
    • IL-5
    • interleukin-5
    • T-cell replacing factor
    • TRF
  • NCBI_Gene: 3567
  • UniProtKB: P05113

Human IL5 Predicted Functions

Predicted to enable cytokine activity; growth factor activity; and interleukin-5 receptor binding activity. Involved in positive regulation of podosome assembly. Located in extracellular region. Implicated in asthma. Biomarker of several diseases, including Churg-Strauss syndrome; Henoch-Schoenlein purpura; arthritis (multiple); asthma (multiple); and lymphoid leukemia (multiple).

Mouse Il5 Target Information

Mouse Il5 Predicted Functions

Enables cytokine activity. Involved in positive regulation of DNA-binding transcription factor activity; positive regulation of immunoglobulin production; and positive regulation of transcription, DNA-templated. Acts upstream of or within positive regulation of peptidyl-tyrosine phosphorylation. Located in extracellular space. Is expressed in colon; thymus; and thymus primordium. Human ortholog(s) of this gene implicated in asthma. Orthologous to human IL5 (interleukin 5).

Rat Il5 Target Information

  • Target Symbol: Il5, interleukin 5
  • Alias:
    • B-cell growth factor II
    • BCGF-II
    • cytotoxic T-lymphocyte inducer
    • eosinophil differentiation factor
    • IL-5
    • Interleukin 5 (colony-stimulating factor eosinophil)
    • Interleukin 5 (colony-stimulating factor, eosinophil)
    • interleukin-5
    • T-cell replacing factor
    • TRF
  • NCBI_Gene: 24497

Rat Il5 Predicted Functions

Enables cytokine activity and interleukin-5 receptor binding activity. Involved in several processes, including positive regulation of B cell proliferation; positive regulation of eosinophil differentiation; and positive regulation of receptor signaling pathway via JAK-STAT. Located in extracellular space. Used to study asthma. Biomarker of several diseases, including allergic rhinitis; esophageal cancer; middle cerebral artery infarction; peptic esophagitis; and prostatic hypertrophy. Human ortholog(s) of this gene implicated in asthma. Orthologous to human IL5 (interleukin 5).

IL5 Target News

    5.561
    Heat-Killed Bifidobacterium longum BBMN68 in Pasteurized Yogurt Alleviates Mugwort Pollen-Induced Allergic Airway Responses through Gut Microbiota Modulation in a Murine Model
    Foods
    2.4
    Lonicerin alleviates ovalbumin-induced asthma of mice via inhibiting EZH2/NF-κB signaling pathway
    EXPERIMENTAL ANIMALS
    2.8
    ADP Ribosylation Factor 6 Relieves Airway Inflammation and Remodeling by Inhibiting Ovalbumin Induced-Epithelial Mesenchymal Transition in Experimental Asthma, Possibly by Regulating of E2F Transcription Factor 8
    IMMUNOLOGICAL INVESTIGATIONS
    5.4
    Baiheqingjin formula reduces inflammation in mice with asthma by inhibiting the PI3K/AKT/NF-κb signaling pathway
    JOURNAL OF ETHNOPHARMACOLOGY
    5.4
    Effects of total alkaloids from Alstonia scholaris (L.) R. Br. on ovalbumin-induced asthma mice
    JOURNAL OF ETHNOPHARMACOLOGY
    6.388
    Transcriptomics reveals apigenin alleviates airway inflammation and epithelial cell apoptosis in allergic asthma via MAPK pathway
    PHYTOTHERAPY RESEARCH
    3.2
    Mitochondrial dynamics modulate the allergic inflammation in a murine model of allergic rhinitis
    Immunity Inflammation and Disease
    Allergic Reaction
    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Eye Diseases (Glaucoma & Cataract)
    Lung Disease (Asthma & COPD)
    Multiple Sclerosis
    Osteoarthrosis & Rheumatoid Arthritis
    Systemic Lupus Erythematosus
    Transplantation

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