Mouse IL-5 ELISA Kit

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EK205

Mouse IL-5 ELISA Kit

$245.00 – $350.00

 DATASHEET
Related Target
Speciesmouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 10 μL, cell culture supernatant: 100 μL
Sensitivity0.78 pg/mL
Array Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery78% – 115%
Average Recovery100%
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SKU: EK205 Category:

ELISA Kit Detail Information

Related Target
Speciesmouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 10 μL, cell culture supernatant: 100 μL
Sensitivity0.78 pg/mL
Array Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery78% – 115%
Average Recovery100%
Intra Precision5.2% – 7.2%
Inter Precision4.3% – 7.4%
PlateDetachable 96-well plate
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-5 monoclonal antibody
Mouse IL-5 freeze-dried standard
IL-5 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IL-5 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-5 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-5 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

IL5

IL5 Target Infomation Overview

  • Target Symbol: IL5, interleukin 5
  • Gene Groups: Interleukins
  • Alias: IL-5; EDF; TRF
  • Alias Names: interleukin-5; T-cell replacing factor; B cell differentiation factor I; eosinophil differentiation factor; colony-stimulating factor, eosinophil; interleukin 5 (colony-stimulating factor, eosinophil)

IL5, interleukin 5 Target Infomation by Species

  • human iconHuman
  • mouse iconMouse
  • rat iconRat

Human IL5 Target Information

  • Target Symbol: IL5, interleukin 5
  • Alias:
    • B cell differentiation factor I
    • B-cell differentiation factor I
    • colony-stimulating factor, eosinophil
    • EDF
    • eosinophil differentiation factor
    • IL-5
    • interleukin-5
    • T-cell replacing factor
    • TRF
  • NCBI_Gene: 3567
  • UniProtKB: P05113

Human IL5 Predicted Functions

Predicted to enable cytokine activity; growth factor activity; and interleukin-5 receptor binding activity. Involved in positive regulation of podosome assembly. Located in extracellular region. Implicated in asthma. Biomarker of several diseases, including Churg-Strauss syndrome; Henoch-Schoenlein purpura; arthritis (multiple); asthma (multiple); and lymphoid leukemia (multiple).

Mouse Il5 Target Information

Mouse Il5 Predicted Functions

Enables cytokine activity. Involved in positive regulation of DNA-binding transcription factor activity; positive regulation of immunoglobulin production; and positive regulation of transcription, DNA-templated. Acts upstream of or within positive regulation of peptidyl-tyrosine phosphorylation. Located in extracellular space. Is expressed in colon; thymus; and thymus primordium. Human ortholog(s) of this gene implicated in asthma. Orthologous to human IL5 (interleukin 5).

Rat Il5 Target Information

  • Target Symbol: Il5, interleukin 5
  • Alias:
    • B-cell growth factor II
    • BCGF-II
    • cytotoxic T-lymphocyte inducer
    • eosinophil differentiation factor
    • IL-5
    • Interleukin 5 (colony-stimulating factor eosinophil)
    • Interleukin 5 (colony-stimulating factor, eosinophil)
    • interleukin-5
    • T-cell replacing factor
    • TRF
  • NCBI_Gene: 24497

Rat Il5 Predicted Functions

Enables cytokine activity and interleukin-5 receptor binding activity. Involved in several processes, including positive regulation of B cell proliferation; positive regulation of eosinophil differentiation; and positive regulation of receptor signaling pathway via JAK-STAT. Located in extracellular space. Used to study asthma. Biomarker of several diseases, including allergic rhinitis; esophageal cancer; middle cerebral artery infarction; peptic esophagitis; and prostatic hypertrophy. Human ortholog(s) of this gene implicated in asthma. Orthologous to human IL5 (interleukin 5).

Products with the Same Target - IL5

Mouse IL-5 High Sensitivity ELISA Kit

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Human IL-5 ELISA Kit

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