Human IL-5 ELISA Kit
$245.00 – $350.00
| Related Target | |
|---|---|
| Species | human |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 50 μL |
| Sensitivity | 0.76 pg/mL |
| Array Range | 7.81 pg/mL – 500 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 91% – 107% |
| Average Recovery | 99% |
ELISA Kit Detail Information
| Related Target | |
|---|---|
| Species | human |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 50 μL |
| Sensitivity | 0.76 pg/mL |
| Array Range | 7.81 pg/mL – 500 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 91% – 107% |
| Average Recovery | 99% |
| Intra Precision | 2.5% – 4.0% |
| Inter Precision | 3.2% – 6.1% |
| Plate | Detachable 96-well plate |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-5 monoclonal antibody Human IL-5 freeze-dried standard IL-5 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-5 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-5 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-5 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Related Targets
IL5
IL5 Target Infomation Overview
- Target Symbol: IL5, interleukin 5
- Gene Groups: Interleukins
- Alias: IL-5; EDF; TRF
- Alias Names: interleukin-5; T-cell replacing factor; B cell differentiation factor I; eosinophil differentiation factor; colony-stimulating factor, eosinophil; interleukin 5 (colony-stimulating factor, eosinophil)
IL5, interleukin 5 Target Infomation by Species
- Human
- Mouse
- Rat
Human IL5 Target Information
- Target Symbol: IL5, interleukin 5
- Alias:
- B cell differentiation factor I
- B-cell differentiation factor I
- colony-stimulating factor, eosinophil
- EDF
- eosinophil differentiation factor
- IL-5
- interleukin-5
- T-cell replacing factor
- TRF
- NCBI_Gene: 3567
- UniProtKB: P05113
Human IL5 Predicted Functions
Predicted to enable cytokine activity; growth factor activity; and interleukin-5 receptor binding activity. Involved in positive regulation of podosome assembly. Located in extracellular region. Implicated in asthma. Biomarker of several diseases, including Churg-Strauss syndrome; Henoch-Schoenlein purpura; arthritis (multiple); asthma (multiple); and lymphoid leukemia (multiple).
Mouse Il5 Target Information
- Target Symbol: Il5, interleukin 5
- Alias:
- Il-5
- NCBI_Gene: 16191
Mouse Il5 Predicted Functions
Enables cytokine activity. Involved in positive regulation of DNA-binding transcription factor activity; positive regulation of immunoglobulin production; and positive regulation of transcription, DNA-templated. Acts upstream of or within positive regulation of peptidyl-tyrosine phosphorylation. Located in extracellular space. Is expressed in colon; thymus; and thymus primordium. Human ortholog(s) of this gene implicated in asthma. Orthologous to human IL5 (interleukin 5).
Rat Il5 Target Information
- Target Symbol: Il5, interleukin 5
- Alias:
- B-cell growth factor II
- BCGF-II
- cytotoxic T-lymphocyte inducer
- eosinophil differentiation factor
- IL-5
- Interleukin 5 (colony-stimulating factor eosinophil)
- Interleukin 5 (colony-stimulating factor, eosinophil)
- interleukin-5
- T-cell replacing factor
- TRF
- NCBI_Gene: 24497
Rat Il5 Predicted Functions
Enables cytokine activity and interleukin-5 receptor binding activity. Involved in several processes, including positive regulation of B cell proliferation; positive regulation of eosinophil differentiation; and positive regulation of receptor signaling pathway via JAK-STAT. Located in extracellular space. Used to study asthma. Biomarker of several diseases, including allergic rhinitis; esophageal cancer; middle cerebral artery infarction; peptic esophagitis; and prostatic hypertrophy. Human ortholog(s) of this gene implicated in asthma. Orthologous to human IL5 (interleukin 5).
Products with the Same Target - IL5
Citations for Human IL-5 ELISA Kit
There are no citations for this product yet.
If you buy this product and mention it in your thesis, we welcome you to provide us with the thesis information. As a thank you, we will give you some gifts, such as discount coupons.
Citations data is updating...
Competitive ELISA: Principles, Methods, and Key Differences
Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.
ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol
Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.
ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects
Learn whether to use serum or plasma for ELISA testing, including preparation protocols, key differences (fibrinogen, clotting factors), and how hemolysis impacts results. Essential guide for accurate immunoassays.
Related products
-
-
- EK9125
- ELISA Kit
Glucagon ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1258
- ELISA Kit
Human Apolipoprotein AII/Apo A2 ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1203
- ELISA Kit
Human Apolipoprotein B/Apo B ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1263
- ELISA Kit
Human 4-1BB/TNFRSF9/CD137 ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK406
- ELISA Kit
Guinea Pig IL-6 ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1109
- ELISA Kit
Human sTNF RI/TNFRSF1A ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
Reviews
There are no reviews yet.