Human IL-2 High Sensitivity ELISA Kit

$380.00$480.00

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TARGETSIL20
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity0.31 pg/mL
Range3.91 pg/mL – 250 pg/mL
Assay Time3.5 h
Recovery101% – 129%
Average Recovery1.22
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity0.31 pg/mL
Range3.91 pg/mL – 250 pg/mL
Assay Time3.5 h
Recovery101% – 129%
Average Recovery1.22
Intra Precision4.2% – 4.9%
Inter-Precision6.0% – 11.1%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-2 monoclonal antibody
Human IL-2 freeze-dried standard
IL-2 detect Antibody
Standard Diluent
HRP-labeled streptavidin
Signal enhancer concentrate
Signal enhancer diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-2 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IL-2 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-2 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IL20

IL20 Target Infomation Overview

  • Target Symbol: IL20, interleukin 20
  • Gene Groups: Interleukins
  • Alias: ZCYTO10; IL10D; IL-20

IL20, interleukin 20 Target Infomation by Species

HumanMouseRat

Human IL20 Target Information

Human IL20 Predicted Functions

Predicted to enable interleukin-20 receptor binding activity. Predicted to be involved in positive regulation of keratinocyte differentiation; positive regulation of tyrosine phosphorylation of STAT protein; and regulation of inflammatory response. Predicted to act upstream of or within positive regulation of osteoclast differentiation. Predicted to be located in extracellular region. Implicated in psoriasis. Biomarker of end stage renal disease and rheumatoid arthritis.

Mouse Il20 Target Information

Mouse Il20 Predicted Functions

Enables cytokine activity; interleukin-20 receptor binding activity; and interleukin-22 receptor binding activity. Acts upstream of or within positive regulation of osteoclast differentiation. Located in extracellular space. Is expressed in Harderian gland; ductus deferens; epididymis; prostate gland; and skin. Human ortholog(s) of this gene implicated in psoriasis. Orthologous to human IL20 (interleukin 20).

Rat Il20 Target Information

Rat Il20 Predicted Functions

Predicted to enable cytokine activity; interleukin-20 receptor binding activity; and interleukin-22 receptor binding activity. Predicted to be involved in signal transduction. Predicted to act upstream of or within positive regulation of osteoclast differentiation. Predicted to be located in extracellular space. Biomarker of end stage renal disease. Human ortholog(s) of this gene implicated in psoriasis. Orthologous to human IL20 (interleukin 20).

IL20 Target News

    Allergic Reaction
    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Eye Diseases (Glaucoma & Cataract)
    Lung Disease (Asthma & COPD)
    Multiple Sclerosis
    Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
    Obesity Diabetes
    Osteoarthrosis & Rheumatoid Arthritis
    Sepsis
    Systemic Lupus Erythematosus
    Transplantation
    Tumor Marker

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