Human IL-2 High Sensitivity ELISA Kit

High Sensitivity ELISA

$380.00 – $480.00

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Datasheet

SDS File

TARGETS	IL20
SPECIES	Human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	0.31 pg/mL
Range	3.91 pg/mL – 250 pg/mL
Assay Time	3.5 h
Recovery	101% – 129%
Average Recovery	1.22

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	0.31 pg/mL
Range	3.91 pg/mL – 250 pg/mL
Assay Time	3.5 h
Recovery	101% – 129%
Average Recovery	1.22
Intra Precision	4.2% – 4.9%
Inter-Precision	6.0% – 11.1%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-2 monoclonal antibody Human IL-2 freeze-dried standard IL-2 detect Antibody Standard Diluent HRP-labeled streptavidin Signal enhancer concentrate Signal enhancer diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-2 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IL-2 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-2 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

IL20

IL20 Target Infomation Overview

Target Symbol: IL20, interleukin 20
Gene Groups: Interleukins
Alias: ZCYTO10; IL10D; IL-20

IL20, interleukin 20 Target Infomation by Species

HumanMouseRat

Human IL20 Target Information

Target Symbol: IL20, interleukin 20
Alias:
- cytokine Zcyto10
- four alpha helix cytokine
- IL-20
- IL10D
- interleukin-20
- MGC96907
- ZCYTO10
NCBI_Gene: 50604
UniProtKB: Q9NYY1

Human IL20 Predicted Functions

Predicted to enable interleukin-20 receptor binding activity. Predicted to be involved in positive regulation of keratinocyte differentiation; positive regulation of tyrosine phosphorylation of STAT protein; and regulation of inflammatory response. Predicted to act upstream of or within positive regulation of osteoclast differentiation. Predicted to be located in extracellular region. Implicated in psoriasis. Biomarker of end stage renal disease and rheumatoid arthritis.

Mouse Il20 Target Information

Target Symbol: Il20, interleukin 20
Alias:
- Zcyto10
NCBI_Gene: 58181

Mouse Il20 Predicted Functions

Enables cytokine activity; interleukin-20 receptor binding activity; and interleukin-22 receptor binding activity. Acts upstream of or within positive regulation of osteoclast differentiation. Located in extracellular space. Is expressed in Harderian gland; ductus deferens; epididymis; prostate gland; and skin. Human ortholog(s) of this gene implicated in psoriasis. Orthologous to human IL20 (interleukin 20).

Rat Il20 Target Information

Target Symbol: Il20, interleukin 20
Alias:
- If2d
- interferon 2d
- interleukin-20
- LOC681112
- similar to interleukin 20
NCBI_Gene: 681112
UniProtKB: D4A2T8

Rat Il20 Predicted Functions

Predicted to enable cytokine activity; interleukin-20 receptor binding activity; and interleukin-22 receptor binding activity. Predicted to be involved in signal transduction. Predicted to act upstream of or within positive regulation of osteoclast differentiation. Predicted to be located in extracellular space. Biomarker of end stage renal disease. Human ortholog(s) of this gene implicated in psoriasis. Orthologous to human IL20 (interleukin 20).