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Mouse IL-20 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 10 μL |
| Sensitivity | 0.87 pg/mL |
| Range | 15.63 pg/mL – 1000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 87% – 124% |
| Average Recovery | 1.05 |
| Intra Precision | 1.1% – 4.3% |
| Inter-Precision | 1.2%-4.2% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-20 monoclonal antibody Human E-Cadherin freeze-dried standard E-Cadherin detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IL-20 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-20 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-20 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
IL20
IL20 Target Infomation Overview
- Target Symbol: IL20, interleukin 20
- Gene Groups: Interleukins
- Alias: ZCYTO10; IL10D; IL-20
IL20, interleukin 20 Target Infomation by Species
HumanMouseRat
Human IL20 Target Information
- Target Symbol: IL20, interleukin 20
- Alias:
- cytokine Zcyto10
- four alpha helix cytokine
- IL-20
- IL10D
- interleukin-20
- MGC96907
- ZCYTO10
- NCBI_Gene: 50604
- UniProtKB: Q9NYY1
Human IL20 Predicted Functions
Predicted to enable interleukin-20 receptor binding activity. Predicted to be involved in positive regulation of keratinocyte differentiation; positive regulation of tyrosine phosphorylation of STAT protein; and regulation of inflammatory response. Predicted to act upstream of or within positive regulation of osteoclast differentiation. Predicted to be located in extracellular region. Implicated in psoriasis. Biomarker of end stage renal disease and rheumatoid arthritis.
Mouse Il20 Target Information
- Target Symbol: Il20, interleukin 20
- Alias:
- Zcyto10
- NCBI_Gene: 58181
Mouse Il20 Predicted Functions
Enables cytokine activity; interleukin-20 receptor binding activity; and interleukin-22 receptor binding activity. Acts upstream of or within positive regulation of osteoclast differentiation. Located in extracellular space. Is expressed in Harderian gland; ductus deferens; epididymis; prostate gland; and skin. Human ortholog(s) of this gene implicated in psoriasis. Orthologous to human IL20 (interleukin 20).
Rat Il20 Target Information
- Target Symbol: Il20, interleukin 20
- Alias:
- If2d
- interferon 2d
- interleukin-20
- LOC681112
- similar to interleukin 20
- NCBI_Gene: 681112
- UniProtKB: D4A2T8
Rat Il20 Predicted Functions
Predicted to enable cytokine activity; interleukin-20 receptor binding activity; and interleukin-22 receptor binding activity. Predicted to be involved in signal transduction. Predicted to act upstream of or within positive regulation of osteoclast differentiation. Predicted to be located in extracellular space. Biomarker of end stage renal disease. Human ortholog(s) of this gene implicated in psoriasis. Orthologous to human IL20 (interleukin 20).
