Rat IL-10 ELISA Kit

$350.00$450.00

TARGETS IL10
SPECIES Rat
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume 20 μL
Sensitivity 0.48 pg/mL
Range 62.5 pg/mL – 4000 pg/mL
Assay Time 3.5 h
Recovery 86% – 108%
Average Recovery 0.98
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume 20 μL
Sensitivity 0.48 pg/mL
Range 62.5 pg/mL – 4000 pg/mL
Assay Time 3.5 h
Recovery 86% – 108%
Average Recovery 0.98
Intra Precision 3.4% – 5.3%
Inter-Precision 4.5% – 6.1%
Platform ELISA
Plate Detachable 96-well plate
Size 96T/48T
Storage If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery 4℃ blue ice transportation
Components 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-10 monoclonal antibody
Rat IL-10 freeze-dried standard
IL-10 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay Principle This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-rat IL-10 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-10 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-10 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IL10

IL10 Target Infomation Overview

  • Target Symbol: IL10, interleukin 10
  • Gene Groups: Interleukins
  • Alias: CSIF; TGIF; IL10A; IL-10
  • Alias Names: cytokine synthesis inhibitory factor; T-cell growth inhibitory factor

IL10, interleukin 10 Target Infomation by Species

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Human IL10 Target Information

  • Target Symbol: IL10, interleukin 10
  • Alias:
    • CSIF
    • cytokine synthesis inhibitory factor
    • GVHDS
    • IL-10
    • IL10A
    • interleukin-10
    • MGC126450
    • MGC126451
    • T-cell growth inhibitory factor
    • TGIF
  • NCBI_Gene: 3586
  • UniProtKB: P22301

Human IL10 Predicted Functions

Enables protein dimerization activity. Involved in several processes, including negative regulation of cellular catabolic process; regulation of gene expression; and regulation of signal transduction. Acts upstream of or within several processes, including negative regulation of B cell proliferation; positive regulation of B cell apoptotic process; and positive regulation of signaling receptor activity. Located in extracellular space. Implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and hematologic cancer (multiple). Biomarker of several diseases, including autoimmune disease (multiple); diabetes mellitus (multiple); eye disease (multiple); female reproductive organ cancer (multiple); and hematologic cancer (multiple).

[/su_tab]
[su_tab title=”Mouse” disabled=”no” anchor=”” url=”” target=”blank” class=”tab-mouse”]

Mouse Il10 Target Information

Mouse Il10 Predicted Functions

Enables cytokine activity. Involved in several processes, including negative regulation of cell death; negative regulation of heterotypic cell-cell adhesion; and negative regulation of vascular associated smooth muscle cell proliferation. Acts upstream of or within several processes, including cellular response to hepatocyte growth factor stimulus; negative regulation of cytokine production; and response to bacterium. Located in extracellular space. Is expressed in colon; liver; thymus; and thymus primordium. Used to study inflammatory bowel disease. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and hematologic cancer (multiple). Orthologous to human IL10 (interleukin 10).

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Rat Il10 Target Information

Rat Il10 Predicted Functions

Enables interleukin-10 receptor binding activity. Involved in several processes, including cellular response to estradiol stimulus; liver regeneration; and negative regulation of nitric oxide biosynthetic process. Located in extracellular space. Used to study several diseases, including lung disease (multiple); perinatal necrotizing enterocolitis; sciatic neuropathy; toxic encephalopathy; and transient cerebral ischemia. Biomarker of several diseases, including artery disease (multiple); decubitus ulcer; lung disease (multiple); obstructive sleep apnea; and postpartum depression. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and hematologic cancer (multiple). Orthologous to human IL10 (interleukin 10).

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IL10 Target News

[catlist tags=”il10″ template=targetnews thumbnail=yes thumbnail_class=”related-post-media clr” thumbnail_size=833]

4
Heat-killed Bifidobacterium longum BBMN68 and inulin protect against high-fat diet-induced obesity by modulating gut microbiota
Frontiers in Nutrition
2.9
Glycoprotein Non-metastatic Melanoma Protein B (GPNMB) Protects Against Neuroinflammation and Neuronal Loss in Pilocarpine-induced Epilepsy via the Regulation of Microglial Polarization
NEUROSCIENCE
4.8
Multi-omics integrated analysis indicated that non-polysaccharides of Sijunzi decoction ameliorated spleen deficiency syndrome via regulating microbiota-gut-metabolites axis and exerted synergistic compatibility
JOURNAL OF ETHNOPHARMACOLOGY
1.5
Maqin Xiaoke Granule Alleviates Acute Bronchitis Induced by Smoking Combined With Streptococcus pneumoniae by Regulating NLRP3/p38 MAPK/Glycerophospholipid Pathway
Natural Product Communications
8.3
Autoantigenic Peptide and Immunomodulator Codelivery System for Rheumatoid Arthritis Treatment by Reestablishing Immune Tolerance
ACS Applied Materials & Interfaces
10.5
ROS-responsive sprayable hydrogel as ROS scavenger and GATA6+ macrophages trap for the prevention of postoperative abdominal adhesions
JOURNAL OF CONTROLLED RELEASE
3.5
Isobavachalcone alleviates ischemic stroke by suppressing HDAC1 expression and improving M2 polarization
BRAIN RESEARCH BULLETIN
4.2
PTPRO inhibition ameliorates spinal cord injury through shifting microglial M1/M2 polarization via the NF-κB/STAT6 signaling pathway
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
4.2
Lilrb4 ameliorates ileal injury in rats with hemorrhagic shock and suppresses the activation of NF-κB signaling pathway
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
13.3
Polyphenol-integrated carboxymethyl chitosan hydrogels with immunoregulatory properties remodeling of inflammatory microenvironment for spinal cord injury repair
CHEMICAL ENGINEERING JOURNAL
5.6
Clostridium butyricum Prevents Dysbiosis and the Rise in Blood Pressure in Spontaneously Hypertensive Rats
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
6.1
M2 macrophages mediate fibrotic scar formation in the early stages after cerebral ischemia in rats
Neural Regeneration Research
5.2
Astaxanthin prevents osteoarthritis by blocking Rspo2-mediated Wnt/β-catenin signaling in chondrocytes and abolishing Rspo2-related inflammatory factors in macrophages
Aging-US
4.8
GRP78 improves the therapeutic effect of mesenchymal stem cells on hemorrhagic shock-induced liver injury: Involvement of the NF–кB and HO-1/Nrf-2 pathways
FASEB JOURNAL
4.1
TNF-α preconditioned UCMSCs-derived EVs enhance the inhibition of necroptosis of acinar cells in SAP
Tissue Engineering Part A
17.1
Tailored Apoptotic Vesicle Delivery Platform for Inflammatory Regulation and Tissue Repair to Ameliorate Ischemic Stroke
ACS Nano
Allergic Reaction
Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
Eye Diseases (Glaucoma & Cataract)
Lung Disease (Asthma & COPD)
Multiple Sclerosis
Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
Obesity Diabetes
Osteoarthrosis & Rheumatoid Arthritis
Osteoporosis
Sepsis
Systemic Lupus Erythematosus
Transplantation
Tumor Marker

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