Human IL-10 High Sensitivity ELISA Kit

$380.00$480.00

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TARGETSIL10
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity0.10 pg/mL
Range0.39 pg/mL – 25 pg/mL
Assay Time3.5 h
Recovery88% – 100%
Average Recovery0.92
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity0.10 pg/mL
Range0.39 pg/mL – 25 pg/mL
Assay Time3.5 h
Recovery88% – 100%
Average Recovery0.92
Intra Precision3.5% – 4.1%
Inter-Precision4.0% – 8.8%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-10 monoclonal antibody
Human IL-10 freeze-dried standard
IL-10 detect Antibody
Standard Diluent
HRP-labeled streptavidin
Signal enhancer concentrate
Signal enhancer diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-10 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IL-10 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-10 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IL10

IL10 Target Infomation Overview

  • Target Symbol: IL10, interleukin 10
  • Gene Groups: Interleukins
  • Alias: CSIF; TGIF; IL10A; IL-10
  • Alias Names: cytokine synthesis inhibitory factor; T-cell growth inhibitory factor

IL10, interleukin 10 Target Infomation by Species

HumanMouseRat

Human IL10 Target Information

  • Target Symbol: IL10, interleukin 10
  • Alias:
    • CSIF
    • cytokine synthesis inhibitory factor
    • GVHDS
    • IL-10
    • IL10A
    • interleukin-10
    • MGC126450
    • MGC126451
    • T-cell growth inhibitory factor
    • TGIF
  • NCBI_Gene: 3586
  • UniProtKB: P22301

Human IL10 Predicted Functions

Enables protein dimerization activity. Involved in several processes, including negative regulation of cellular catabolic process; regulation of gene expression; and regulation of signal transduction. Acts upstream of or within several processes, including negative regulation of B cell proliferation; positive regulation of B cell apoptotic process; and positive regulation of signaling receptor activity. Located in extracellular space. Implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and hematologic cancer (multiple). Biomarker of several diseases, including autoimmune disease (multiple); diabetes mellitus (multiple); eye disease (multiple); female reproductive organ cancer (multiple); and hematologic cancer (multiple).

Mouse Il10 Target Information

Mouse Il10 Predicted Functions

Enables cytokine activity. Involved in several processes, including negative regulation of cell death; negative regulation of heterotypic cell-cell adhesion; and negative regulation of vascular associated smooth muscle cell proliferation. Acts upstream of or within several processes, including cellular response to hepatocyte growth factor stimulus; negative regulation of cytokine production; and response to bacterium. Located in extracellular space. Is expressed in colon; liver; thymus; and thymus primordium. Used to study inflammatory bowel disease. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and hematologic cancer (multiple). Orthologous to human IL10 (interleukin 10).

Rat Il10 Target Information

Rat Il10 Predicted Functions

Enables interleukin-10 receptor binding activity. Involved in several processes, including cellular response to estradiol stimulus; liver regeneration; and negative regulation of nitric oxide biosynthetic process. Located in extracellular space. Used to study several diseases, including lung disease (multiple); perinatal necrotizing enterocolitis; sciatic neuropathy; toxic encephalopathy; and transient cerebral ischemia. Biomarker of several diseases, including artery disease (multiple); decubitus ulcer; lung disease (multiple); obstructive sleep apnea; and postpartum depression. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and hematologic cancer (multiple). Orthologous to human IL10 (interleukin 10).

IL10 Target News

    3.1
    The Effect of an Extract of Sappanwood, Protosappanin A and Protosappanin B on Osteogenesis in Periodontitis
    Frontiers in Bioscience-Landmark
    2.8
    Characteristics of Serum Autoantibody Repertoire and Immune Subgroup Variation of Tuberculosis-Associated Obstructive Pulmonary Disease
    International Journal of Chronic Obstructive Pulmonary Disease
    7.259
    Azithromycin attenuates wheezing after pulmonary inflammation through inhibiting histone H3K27me3 hypermethylation mediated by EZH2
    Clinical Epigenetics
    4.047
    Clonorchis sinensis granulin promotes malignant transformation of human intrahepatic biliary epithelial cells through interaction with M2 macrophages via regulation of STAT3  phosphorylation and the MEK/ERK pathway
    Parasites & Vectors
    15.9
    CAP2 promotes gastric cancer metastasis by mediating the interaction between tumor cells and tumor-associated macrophages
    JOURNAL OF CLINICAL INVESTIGATION
    8
    Single-cell transcriptomics reveals variations in monocytes and Tregs between gout flare and remission
    JCI Insight
    4.7
    α5-nAChR/STAT3/CD47 axis contributed to nicotine-related lung adenocarcinoma progression and immune escape
    CARCINOGENESIS
    4.6
    Orosomucoid 1 promotes colorectal cancer progression and liver metastasis by affecting PI3K/AKT pathway and inducing macrophage M2 polarization
    Scientific Reports
    5.195
    Modified Gegen Qinlian decoction ameliorated ulcerative colitis by attenuating inflammation and oxidative stress and enhancing intestinal barrier function in vivo and in vitro
    JOURNAL OF ETHNOPHARMACOLOGY
    5.714
    Parthenolide alleviates microglia‐mediated neuroinflammation via MAPK/TRIM31/NLRP3 signaling to ameliorate cognitive disorder
    INTERNATIONAL IMMUNOPHARMACOLOGY
    5.6
    The enrichment of Arg1+ILC2s and ILCregs facilitates the progression of endometriosis: A preliminary study
    INTERNATIONAL IMMUNOPHARMACOLOGY
    3.7
    A role of NR4A2 in Graves’ disease: regulation of Th17/Treg
    ENDOCRINE
    5.8
    BMI-specific inflammatory response to phthalate exposure in early pregnancy: findings from the TMCHESC study
    ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
    5.1
    hPMSCs Regulate the Level of TNF-α and IL-10 in Th1 Cells and Improve Hepatic Injury in a GVHD Mouse Model via CD73/ADO/Fyn/Nrf2 Axis
    INFLAMMATION
    5.139
    Potentiated effects of lactate receptor GPR81 on immune microenvironment in breast cancer
    MOLECULAR CARCINOGENESIS
    3.124
    MicroRNA-143-3p ameliorates collagen-induced arthritis by polarizing naive CD4+T cells into Treg cells
    JOURNAL OF CLINICAL LABORATORY ANALYSIS
    Allergic Reaction
    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Eye Diseases (Glaucoma & Cataract)
    Lung Disease (Asthma & COPD)
    Multiple Sclerosis
    Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
    Obesity Diabetes
    Osteoarthrosis & Rheumatoid Arthritis
    Osteoporosis
    Sepsis
    Systemic Lupus Erythematosus
    Transplantation
    Tumor Marker

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