Rat IL-10 High Sensitivity ELISA Kit

High Sensitivity ELISA

$380.00 – $480.00

Download:

Datasheet

SDS File

TARGETS	IL10
SPECIES	Rat
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	10 μL
Sensitivity	0.22 pg/mL
Range	6.25 pg/mL – 400 pg/mL
Assay Time	3.5 h
Recovery	94% – 115%
Average Recovery	1.03

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	10 μL
Sensitivity	0.22 pg/mL
Range	6.25 pg/mL – 400 pg/mL
Assay Time	3.5 h
Recovery	94% – 115%
Average Recovery	1.03
Intra Precision	4.4% – 4.8%
Inter-Precision	3.9% – 4.9%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-10 monoclonal antibody Rat IL-10 freeze-dried standard IL-10 detect Antibody Standard Diluent HRP-labeled streptavidin Signal enhancer concentrate Signal enhancer diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-rat IL-10 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IL-10 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-10 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

IL10

IL10 Target Infomation Overview

Target Symbol: IL10, interleukin 10
Gene Groups: Interleukins
Alias: CSIF; TGIF; IL10A; IL-10
Alias Names: cytokine synthesis inhibitory factor; T-cell growth inhibitory factor

IL10, interleukin 10 Target Infomation by Species

HumanMouseRat

Human IL10 Target Information

Target Symbol: IL10, interleukin 10
Alias:
- CSIF
- cytokine synthesis inhibitory factor
- GVHDS
- IL-10
- IL10A
- interleukin-10
- MGC126450
- MGC126451
- T-cell growth inhibitory factor
- TGIF
NCBI_Gene: 3586
UniProtKB: P22301

Human IL10 Predicted Functions

Enables protein dimerization activity. Involved in several processes, including negative regulation of cellular catabolic process; regulation of gene expression; and regulation of signal transduction. Acts upstream of or within several processes, including negative regulation of B cell proliferation; positive regulation of B cell apoptotic process; and positive regulation of signaling receptor activity. Located in extracellular space. Implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and hematologic cancer (multiple). Biomarker of several diseases, including autoimmune disease (multiple); diabetes mellitus (multiple); eye disease (multiple); female reproductive organ cancer (multiple); and hematologic cancer (multiple).

Mouse Il10 Target Information

Target Symbol: Il10, interleukin 10
Alias:
- cytokine synthesis inhibitory factor
- IL-10
- Il-10
NCBI_Gene: 16153

Mouse Il10 Predicted Functions

Enables cytokine activity. Involved in several processes, including negative regulation of cell death; negative regulation of heterotypic cell-cell adhesion; and negative regulation of vascular associated smooth muscle cell proliferation. Acts upstream of or within several processes, including cellular response to hepatocyte growth factor stimulus; negative regulation of cytokine production; and response to bacterium. Located in extracellular space. Is expressed in colon; liver; thymus; and thymus primordium. Used to study inflammatory bowel disease. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and hematologic cancer (multiple). Orthologous to human IL10 (interleukin 10).

Rat Il10 Target Information

Target Symbol: Il10, interleukin 10
Alias:
- CSIF
- cytokine synthesis inhibitory factor
- If2a
- IL-10
- IL10X
- interferon 2a
- interleukin-10
NCBI_Gene: 25325
- UniprotKB: A0A7R8C3K4
- UniprotKB: P29456

Rat Il10 Predicted Functions

Enables interleukin-10 receptor binding activity. Involved in several processes, including cellular response to estradiol stimulus; liver regeneration; and negative regulation of nitric oxide biosynthetic process. Located in extracellular space. Used to study several diseases, including lung disease (multiple); perinatal necrotizing enterocolitis; sciatic neuropathy; toxic encephalopathy; and transient cerebral ischemia. Biomarker of several diseases, including artery disease (multiple); decubitus ulcer; lung disease (multiple); obstructive sleep apnea; and postpartum depression. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and hematologic cancer (multiple). Orthologous to human IL10 (interleukin 10).