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Mouse CCL20/MIP-3α ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 20 μL |
| Sensitivity | 5.82 pg/mL |
| Range | 62.5 pg/mL – 4000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 82% – 96% |
| Average Recovery | 0.9 |
| Intra Precision | 4.5% – 7.5% |
| Inter-Precision | 5.6% – 6.9% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CCL20/MIP-3α monoclonal antibody Mouse CCL20/MIP-3α freeze-dried standard CCL20/MIP-3α detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) technique. Monoclonal antibodies specific for mouse CCL20 are pre-coated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, the CCL20 present in the sample binds to the solid-phase antibody and the detection antibody. After washing to remove unbound substances, streptavidin-HRP is added. After washing again, the TMB chromogenic substrate is added and color development is carried out while avoiding light. The intensity of the color reaction is directly proportional to the concentration of CCL20 in the sample. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
CCL20
CCL20 Target Infomation Overview
- Target Symbol: CCL20, C-C motif chemokine ligand 20
- Gene Groups: Chemokine ligands
- Alias: LARC; MIP-3a; exodus-1; ST38; CKb4
- Previous Names: SCYA20
- Alias Names: small inducible cytokine subfamily A (Cys-Cys), member 20; chemokine (C-C motif) ligand 20
CCL20, C-C motif chemokine ligand 20 Target Infomation by Species
HumanMouseRat
Human CCL20 Target Information
- Target Symbol: CCL20, C-C motif chemokine ligand 20
- Alias:
- beta chemokine exodus-1
- beta-chemokine exodus-1
- C-C motif chemokine 20
- CC chemokine LARC
- chemokine (C-C motif) ligand 20
- CKb4
- Exodus
- exodus-1
- LARC
- liver and activation-regulated chemokine
- macrophage inflammatory protein 3 alpha
- MIP-3-alpha
- MIP-3a
- MIP3A
- SCYA20
- small inducible cytokine subfamily A (Cys-Cys), member 20
- small-inducible cytokine A20
- ST38
- NCBI_Gene: 6364
- UniProtKB: P78556
Human CCL20 Predicted Functions
Enables CCR6 chemokine receptor binding activity. Involved in several processes, including antimicrobial humoral immune response mediated by antimicrobial peptide; calcium-mediated signaling using intracellular calcium source; and cell chemotaxis. Acts upstream of or within positive regulation of T cell migration. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Biomarker of several diseases, including IgA glomerulonephritis; Lyme disease; anogenital venereal wart; dermatitis (multiple); and skin papilloma.
Mouse Ccl20 Target Information
- Target Symbol: Ccl20, chemokine (C-C motif) ligand 20
- Alias:
- CKb4
- exodus-1
- MIP-3[a]
- MIP-3A
- MIP3A
- Scya20
- small inducible cytokine subfamily A20
- ST38
- NCBI_Gene: 20297
Mouse Ccl20 Predicted Functions
Enables CCR6 chemokine receptor binding activity and cytokine activity. Involved in chemotaxis and thymocyte migration. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Orthologous to human CCL20 (C-C motif chemokine ligand 20).
Rat Ccl20 Target Information
- Target Symbol: Ccl20, C-C motif chemokine ligand 20
- Alias:
- beta chemokine exodus-1
- beta-chemokine exodus-1
- C-C motif chemokine 20
- CC chemokine LARC
- CC chemokine ST38
- chemokine (C-C motif) ligand 20
- liver and activation-regulated chemokine
- macrophage inflammatory protein 3 alpha
- MIP-3-alpha
- Scya20
- small inducible cytokine subfamily A20
- small-inducible cytokine A20
- ST38
- NCBI_Gene: 29538
- UniProtKB: P97884
Rat Ccl20 Predicted Functions
Enables chemokine activity. Involved in several processes, including cellular response to molecule of bacterial origin; positive regulation of macromolecule metabolic process; and wound healing. Located in extracellular space. Used to study transient cerebral ischemia. Biomarker of brain ischemia and ulcerative colitis. Orthologous to human CCL20 (C-C motif chemokine ligand 20).
