Human Apolipoprotein E/Apo E ELISA Kit

$350.00$450.00

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TARGETSAPOE
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume90 μL 1×Assay Buffer and 10 μL prediluted sample
Sensitivity1.71 pg/mL
Range78.13 pg/mL – 5000 pg/mL
Assay Time3.5 h
Recovery85% – 117%
Average Recovery1.08
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume90 μL 1×Assay Buffer and 10 μL prediluted sample
Sensitivity1.71 pg/mL
Range78.13 pg/mL – 5000 pg/mL
Assay Time3.5 h
Recovery85% – 117%
Average Recovery1.08
Intra Precision3.5% – 5.0%
Inter-Precision3.8% – 5.0%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Apolipoprotein E/Apo E monoclonal antibody
Human Apolipoprotein E/Apo E freeze-dried standard
Apolipoprotein E/Apo E detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Apo E antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Apo E present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Apo E in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

APOE

APOE Target Infomation Overview

  • Target Symbol: APOE, apolipoprotein E
  • Gene Groups: Apolipoproteins
  • Previous Names: AD2
  • Alias Names: Alzheimer disease 2 (APOE*E4-associated, late onset)

APOE, apolipoprotein E Target Infomation by Species

HumanMouseRat

Human APOE Target Information

  • Target Symbol: APOE, apolipoprotein E
  • Alias:
    • AD2
    • Alzheimer disease 2 (APOE*E4-associated, late onset)
    • apo-E
    • ApoE4
    • apolipoprotein E3
    • LDLCQ5
    • LPG
    • MGC1571
  • NCBI_Gene: 348
  • UniProtKB: P02649

Human APOE Predicted Functions

Enables several functions, including amyloid-beta binding activity; heparan sulfate proteoglycan binding activity; and lipoprotein particle receptor binding activity. Involved in several processes, including lipid transport; regulation of lipid transport; and regulation of protein metabolic process. Acts upstream of or within with a positive effect on AMPA glutamate receptor clustering and NMDA glutamate receptor clustering. Acts upstream of or within negative regulation of dendritic spine development; positive regulation of dendritic spine development; and regulation of dendritic spine maintenance. Located in several cellular components, including Golgi apparatus; endoplasmic reticulum; and extracellular space. Part of several cellular components, including chylomicron; low-density lipoprotein particle; and triglyceride-rich plasma lipoprotein particle. Is active in glutamatergic synapse and synaptic cleft. Implicated in several diseases, including Alzheimer’s disease (multiple); artery disease (multiple); biliary tract cancer (multiple); eye disease (multiple); and familial hyperlipidemia (multiple). Biomarker of several diseases, including Lewy body dementia; diabetes mellitus (multiple); end stage renal disease; neurodegenerative disease (multiple); and obesity.

Mouse Apoe Target Information

Mouse Apoe Predicted Functions

Enables several functions, including cholesterol transfer activity; heparan sulfate proteoglycan binding activity; and phosphatidylcholine-sterol O-acyltransferase activator activity. Involved in several processes, including negative regulation of macromolecule metabolic process; plasma lipoprotein particle remodeling; and regulation of defense response. Acts upstream of or within with a negative effect on gene expression. Acts upstream of or within several processes, including cholesterol catabolic process; cholesterol efflux; and lipoprotein metabolic process. Located in extracellular space and glutamatergic synapse. Part of low-density lipoprotein particle. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; liver and biliary system; and sensory organ. Human ortholog(s) of this gene implicated in several diseases, including Alzheimer’s disease (multiple); artery disease (multiple); biliary tract cancer (multiple); eye disease (multiple); and familial hyperlipidemia (multiple). Orthologous to human APOE (apolipoprotein E).

Rat Apoe Target Information

Rat Apoe Predicted Functions

Enables several functions, including amyloid-beta binding activity; hydroxyapatite binding activity; and phospholipid binding activity. Involved in several processes, including cellular response to alcohol; neurogenesis; and regulation of lipid metabolic process. Located in several cellular components, including late endosome; neuronal cell body; and nuclear envelope. Is extrinsic component of external side of plasma membrane. Part of several cellular components, including discoidal high-density lipoprotein particle; low-density lipoprotein particle; and triglyceride-rich plasma lipoprotein particle. Colocalizes with early endosome and microtubule. Used to study artery disease (multiple); familial hyperlipidemia; fatty liver disease (multiple); glomerulosclerosis; and middle cerebral artery infarction. Biomarker of several diseases, including glomerulonephritis (multiple); hyperhomocysteinemia; hypertension; sciatic neuropathy; and transient cerebral ischemia. Human ortholog(s) of this gene implicated in several diseases, including Alzheimer’s disease (multiple); artery disease (multiple); biliary tract cancer (multiple); eye disease (multiple); and familial hyperlipidemia (multiple). Orthologous to human APOE (apolipoprotein E).

APOE Target News

    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Multiple Sclerosis
    Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
    Obesity Diabetes

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