Human CCL27/CTACK ELISA Kit

$350.00$450.00

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TARGETSCCL27
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL 1×Assay Buffer and 50 μL sample
Sensitivity1.85 pg/mL
Range93.75 pg/mL – 6000 pg/mL
Assay Time3.5 h
Recovery86% – 108%
Average Recovery0.92
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL 1×Assay Buffer and 50 μL sample
Sensitivity1.85 pg/mL
Range93.75 pg/mL – 6000 pg/mL
Assay Time3.5 h
Recovery86% – 108%
Average Recovery0.92
Intra Precision4.4% – 5.2%
Inter-Precision2.2% – 2.6%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CCL27/CTACK monoclonal antibody
Human CCL27/CTACK freeze-dried standard
CCL27/CTACK detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human CCL27 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, CCL27 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CCL27 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

CCL27

CCL27 Target Infomation Overview

  • Target Symbol: CCL27, C-C motif chemokine ligand 27
  • Gene Groups: Chemokine ligands
  • Alias: ALP; ILC; CTACK; skinkine; ESkine; PESKY; CTAK
  • Previous Names: SCYA27
  • Alias Names: CC chemokine ILC; IL-11 Ralpha-locus chemokine; cutaneous T-cell attracting chemokine; small inducible cytokine subfamily A (Cys-Cys), member 27; chemokine (C-C motif) ligand 27

CCL27, C-C motif chemokine ligand 27 Target Infomation by Species

HumanMouseRat

Human CCL27 Target Information

  • Target Symbol: CCL27, C-C motif chemokine ligand 27
  • Alias:
    • ALP
    • C-C motif chemokine 27
    • CC chemokine ILC
    • chemokine (C-C motif) ligand 27
    • CTACK
    • CTAK
    • cutaneous T-cell attracting chemokine
    • cutaneous T-cell-attracting chemokine
    • ESKINE
    • IL-11 R-alpha-locus chemokine
    • IL-11 Ralpha-locus chemokine
    • ILC
    • PESKY
    • SCYA27
    • skinkine
    • small inducible cytokine subfamily A (Cys-Cys), member 27
    • small-inducible cytokine A27
  • NCBI_Gene: 10850
  • UniProtKB: Q9Y4X3

Human CCL27 Predicted Functions

Enables CCR3 chemokine receptor binding activity. Involved in antimicrobial humoral immune response mediated by antimicrobial peptide and killing of cells of other organism. Predicted to be located in nucleus. Predicted to be active in extracellular region. Biomarker of COVID-19; asthma; and atopic dermatitis.

Mouse Ccl27a Target Information

  • Target Symbol: Ccl27a, chemokine (C-C motif) ligand 27A
  • Alias:
    • ALP
    • AW558992
    • Ccl27
    • chemokine (C-C motif) ligand 27
    • chemokine (C-C motif) ligand 27a
    • CTACK
    • CTAK
    • ESkine
    • expressed sequence AW558992
    • ILC
    • mILC
    • PESKY
    • Scya27
    • Scya27a
    • skinskine
    • small inducible cytokine A27
    • small inducible cytokine A27a
  • NCBI_Gene: 20301

Mouse Ccl27a Predicted Functions

Enables chemokine activity. Involved in cell chemotaxis. Acts upstream of or within positive regulation of T cell chemotaxis and positive regulation of actin cytoskeleton reorganization. Located in extracellular region and nucleus. Is expressed in several structures, including genitourinary system; hip; liver; sensory organ; and skeleton. Orthologous to human CCL27 (C-C motif chemokine ligand 27).

Rat Ccl27 Target Information

Rat Ccl27 Predicted Functions

Predicted to enable CCR3 chemokine receptor binding activity and chemokine activity. Predicted to be involved in several processes, including antimicrobial humoral immune response mediated by antimicrobial peptide; positive regulation of T cell chemotaxis; and positive regulation of actin cytoskeleton reorganization. Predicted to act upstream of or within positive regulation of mononuclear cell migration. Predicted to be located in nucleus. Predicted to be active in extracellular region. Orthologous to human CCL27 (C-C motif chemokine ligand 27).

CCL27 Target News

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