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Human P4HB ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 20 μL |
| Sensitivity | 7.77 pg/mL |
| Range | 0.16 ng/mL – 10 ng/mL |
| Assay Time | 3.5 h |
| Recovery | 77% – 104% |
| Average Recovery | 0.93 |
| Intra Precision | 2.5% – 3.9% |
| Inter-Precision | 1.6%-6.2% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-P4HB monoclonal antibody Human P4HB freeze-dried standard Human P4HB detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human P4HB antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, P4HB present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of P4HB in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
P4HB
P4HB Target Infomation Overview
- Target Symbol: P4HB, prolyl 4-hydroxylase subunit beta
- Gene Groups: Protein disulfide isomerases
- Alias: PDIA1; PROHB; DSI; GIT; PDI; PO4HB; P4Hbeta
- Previous Names: PO4DB; ERBA2L
- Alias Names: protein disulfide isomerase-associated 1; protein disulfide isomerase family A, member 1; collagen prolyl 4-hydroxylase beta; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide (protein disulfide isomerase; thyroid hormone binding protein p55); procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide (protein disulfide isomerase-associated 1); procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide; prolyl 4-hydroxylase, beta polypeptide
P4HB, prolyl 4-hydroxylase subunit beta Target Infomation by Species
HumanMouseRat
Human P4HB Target Information
- Target Symbol: P4HB, prolyl 4-hydroxylase subunit beta
- Alias:
- cellular thyroid hormone-binding protein
- CLCRP1
- collagen prolyl 4-hydroxylase beta
- DSI
- ERBA2L
- GIT
- glutathione-insulin transhydrogenase
- P4Hbeta
- p55
- PDI
- PDIA1
- PHDB
- PO4DB
- PO4HB
- procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide
- PROHB
- prolyl 4-hydroxylase, beta polypeptide
- protein disulfide isomerase family A, member 1
- protein disulfide isomerase-associated 1
- protein disulfide isomerase/oxidoreductase
- protein disulfide-isomerase
- protocollagen hydroxylase
- testicular secretory protein Li 32
- thyroid hormone-binding protein p55
- v-erb-a avian erythroblastic leukemia viral oncogene homolog 2-like
- NCBI_Gene: 5034
- UniProtKB: P07237
Human P4HB Predicted Functions
Enables several functions, including oxidoreductase activity, acting on a sulfur group of donors; protein disulfide isomerase activity; and protein heterodimerization activity. Contributes to procollagen-proline 4-dioxygenase activity. Involved in several processes, including positive regulation of substrate adhesion-dependent cell spreading; positive regulation of viral entry into host cell; and protein folding in endoplasmic reticulum. Acts upstream of or within peptidyl-proline hydroxylation to 4-hydroxy-L-proline. Located in several cellular components, including endoplasmic reticulum-Golgi intermediate compartment; external side of plasma membrane; and lamellipodium. Part of procollagen-proline 4-dioxygenase complex. Implicated in Cole-Carpenter syndrome.
Mouse P4hb Target Information
- Target Symbol: P4hb, prolyl 4-hydroxylase, beta polypeptide
- Alias:
- ERp59
- PDI
- Pdia1
- protein disulfide isomerase
- Thbp
- thyroid hormone binding protein
- NCBI_Gene: 18453
Mouse P4hb Predicted Functions
Enables integrin binding activity. Acts upstream of or within cellular response to interleukin-7. Located in endoplasmic reticulum and external side of plasma membrane. Part of endoplasmic reticulum chaperone complex. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; heart; and sensory organ. Human ortholog(s) of this gene implicated in Cole-Carpenter syndrome. Orthologous to human P4HB (prolyl 4-hydroxylase subunit beta).
Rat P4hb Target Information
- Target Symbol: P4hb, prolyl 4-hydroxylase subunit beta
- Alias:
- cellular thyroid hormone-binding protein
- PDI
- PDIR
- prolyl 4-hydroxylase, beta polypeptide
- protein disulfide isomerase
- protein disulfide isomerase (Prolyl 4-hydroxylase, beta polypeptide)
- protein disulfide-isomerase
- NCBI_Gene: 25506
- UniProtKB: P04785
Rat P4hb Predicted Functions
Enables enzyme binding activity and protein disulfide isomerase activity. Predicted to be involved in several processes, including positive regulation of substrate adhesion-dependent cell spreading; positive regulation of viral entry into host cell; and protein folding in endoplasmic reticulum. Predicted to act upstream of or within cellular response to interleukin-7 and peptidyl-proline hydroxylation to 4-hydroxy-L-proline. Predicted to be located in several cellular components, including cytosol; endoplasmic reticulum-Golgi intermediate compartment; and lamellipodium. Predicted to be part of endoplasmic reticulum chaperone complex and procollagen-proline 4-dioxygenase complex. Predicted to be active in endoplasmic reticulum and external side of plasma membrane. Human ortholog(s) of this gene implicated in Cole-Carpenter syndrome. Orthologous to human P4HB (prolyl 4-hydroxylase subunit beta).
