Human PD-1 ELISA Kit

$350.00$450.00

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TARGETSPDCD1
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity8.62 pg/mL
Range93.75 pg/mL – 6000 pg/mL
Assay Time3.5 h
Recovery95% – 118%
Average Recovery1.07
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity8.62 pg/mL
Range93.75 pg/mL – 6000 pg/mL
Assay Time3.5 h
Recovery95% – 118%
Average Recovery1.07
Intra Precision4.2% – 4.6%
Inter-Precision4.8% – 4.9%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-PD-1 monoclonal antibody
Human PD-1 freeze-dried standard
HRP标记的抗Human PD-1 detect Antibody
Standard Diluent
HRP-labeled streptavidin
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human PD-1单克隆 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, PD-1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of PD-1 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

PDCD1

PDCD1 Target Infomation Overview

  • Target Symbol: PDCD1, programmed cell death 1
  • Gene Groups: CD molecules; V-set domain containing
  • Alias: CD279; PD1; hSLE1; PD-1
  • Previous Names: SLEB2
  • Alias Names: systemic lupus erythematosus susceptibility 2

PDCD1, programmed cell death 1 Target Infomation by Species

HumanMouseRat

Human PDCD1 Target Information

  • Target Symbol: PDCD1, programmed cell death 1
  • Alias:
    • CD279
    • hPD-1
    • hPD-l
    • hSLE1
    • PD-1
    • PD1
    • programmed cell death protein 1
    • SLEB2
    • systemic lupus erythematosus susceptibility 2
  • NCBI_Gene: 5133
  • UniProtKB: Q15116

Human PDCD1 Predicted Functions

Involved in positive regulation of T cell apoptotic process. Predicted to be located in plasma membrane. Predicted to be active in external side of plasma membrane. Implicated in autoimmune disease (multiple); hepatitis B; hepatitis C; hepatocellular carcinoma; and lupus nephritis. Biomarker of several diseases, including Cryptococcal meningitis; anogenital venereal wart; cervix uteri carcinoma in situ; liver disease (multiple); and tuberculosis (multiple).

Mouse Pdcd1 Target Information

Mouse Pdcd1 Predicted Functions

Involved in negative regulation of immune response. Acts upstream of or within negative regulation of apoptotic process; negative regulation of tolerance induction; and positive regulation of apoptotic process. Located in external side of plasma membrane. Is expressed in retina. Used to study dilated cardiomyopathy and systemic lupus erythematosus. Human ortholog(s) of this gene implicated in autoimmune disease (multiple); hepatitis B; hepatitis C; hepatocellular carcinoma; and lupus nephritis. Orthologous to human PDCD1 (programmed cell death 1).

Rat Pdcd1 Target Information

  • Target Symbol: Pdcd1, programmed cell death 1
  • Alias:
    • LOC102550808
    • LOC301626
    • programmed cell death protein 1
    • programmed cell death protein 1-like
  • NCBI_Gene: 301626
  • UniProtKB: D3ZIN8

Rat Pdcd1 Predicted Functions

Predicted to be involved in negative regulation of immune response and positive regulation of T cell apoptotic process. Predicted to act upstream of or within negative regulation of apoptotic process; negative regulation of tolerance induction; and positive regulation of apoptotic process. Located in plasma membrane. Biomarker of anti-basement membrane glomerulonephritis; periodontitis; and pre-eclampsia. Human ortholog(s) of this gene implicated in autoimmune disease (multiple); hepatitis B; hepatitis C; hepatocellular carcinoma; and lupus nephritis. Orthologous to human PDCD1 (programmed cell death 1).

PDCD1 Target News

    Multiple Sclerosis
    Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
    Obesity Diabetes
    Osteoarthrosis & Rheumatoid Arthritis
    Sepsis
    Systemic Lupus Erythematosus
    Transplantation
    Tumor Marker

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