Human Pentraxin 3/TSG-14 ELISA Kit

$350.00$450.00

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TARGETSPTX3
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity0.56 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery75% – 117%
Average Recovery0.93
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity0.56 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery75% – 117%
Average Recovery0.93
Intra Precision2.9% – 6.3%
Inter-Precision2.9% – 3.8%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Pentraxin 3/TSG-14 monoclonal antibody
Human Pentraxin 3/TSG-14 freeze-dried standard
Pentraxin 3/TSG-14 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Pentraxin 3 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Pentraxin 3 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Pentraxin 3 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

PTX3

PTX3 Target Infomation Overview

  • Target Symbol: PTX3, pentraxin 3
  • Gene Groups: Long pentraxins
  • Alias: TSG-14
  • Previous Names: TNFAIP5
  • Alias Names: long pentraxin 3; pentaxin-related gene, rapidly induced by IL-1 beta; tumor necrosis factor, alpha-induced protein 5; pentraxin-related gene, rapidly induced by IL-1 beta; pentraxin 3, long

PTX3, pentraxin 3 Target Infomation by Species

HumanMouseRat

Human PTX3 Target Information

  • Target Symbol: PTX3, pentraxin 3
  • Alias:
    • long pentraxin 3
    • pentaxin-related gene, rapidly induced by il-1 beta
    • pentaxin-related gene, rapidly induced by IL-1 beta, tumor necrosis factor, alpha-induced protein 5
    • pentaxin-related protein PTX3
    • pentraxin 3, long
    • pentraxin-3
    • pentraxin-related gene, rapidly induced by IL-1 beta
    • pentraxin-related protein PTX3
    • TNF alpha-induced protein 5
    • TNFAIP5
    • TSG-14
    • tumor necrosis factor alpha-induced protein 5
    • tumor necrosis factor-inducible gene 14 protein
    • tumor necrosis factor-inducible protein TSG-14
    • tumor necrosis factor, alpha-induced protein 5
  • NCBI_Gene: 5806
  • UniProtKB: P26022

Human PTX3 Predicted Functions

Enables complement component C1q complex binding activity; identical protein binding activity; and virion binding activity. Involved in innate immune response; modulation by host of viral process; and negative regulation of viral entry into host cell. Located in extracellular space. Implicated in cystic fibrosis and invasive aspergillosis. Biomarker of hepatitis C; invasive aspergillosis; pelvic inflammatory disease; pulmonary aspergilloma; and toxic shock syndrome.

Mouse Ptx3 Target Information

Mouse Ptx3 Predicted Functions

Enables (1->3)-beta-D-glucan binding activity. Acts upstream of or within several processes, including opsonization; ovarian cumulus expansion; and positive regulation of nitric oxide biosynthetic process. Located in extracellular matrix. Is expressed in cochlea; cochlea epithelium; inner ear vestibular component; organ of Corti; and stria vascularis. Human ortholog(s) of this gene implicated in cystic fibrosis and invasive aspergillosis. Orthologous to human PTX3 (pentraxin 3).

Rat Ptx3 Target Information

  • Target Symbol: Ptx3, pentraxin 3
  • Alias:
    • LOC689388
    • pentraxin 3, long
    • pentraxin related
    • pentraxin related gene
    • pentraxin-related protein PTX3
    • similar to Pentraxin-related protein PTX3 precursor (Pentaxin-related protein PTX3) (Tumor necrosis factor-inducible protein TSG-14)
  • NCBI_Gene: 689388
  • UniProtKB: D3ZT94

Rat Ptx3 Predicted Functions

Predicted to enable several functions, including (1->3)-beta-D-glucan binding activity; complement component C1q complex binding activity; and virion binding activity. Predicted to be involved in innate immune response; modulation by host of viral process; and negative regulation of viral entry into host cell. Predicted to act upstream of or within several processes, including opsonization; ovarian cumulus expansion; and positive regulation of nitric oxide biosynthetic process. Predicted to be located in extracellular matrix. Predicted to be active in extracellular space. Human ortholog(s) of this gene implicated in cystic fibrosis and invasive aspergillosis. Orthologous to human PTX3 (pentraxin 3).

PTX3 Target News

    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Sepsis

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