Name: Human Pentraxin 3/TSG-14 ELISA Kit
SKU: EK1157
Availability: InStock

EK1157

Human Pentraxin 3/TSG-14 ELISA Kit

$245.00 – $350.00

Related Target	PTX3
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	10 μL
Sensitivity	0.56 pg/mL
Array Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	75% – 117%
Average Recovery	93%

ELISA Kit Detail Information

Related Target	PTX3
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	10 μL
Sensitivity	0.56 pg/mL
Array Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	75% – 117%
Average Recovery	93%
Intra Precision	2.9% – 6.3%
Inter Precision	2.9% – 3.8%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Pentraxin 3/TSG-14 monoclonal antibody Human Pentraxin 3/TSG-14 freeze-dried standard Pentraxin 3/TSG-14 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Pentraxin 3 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Pentraxin 3 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Pentraxin 3 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

PTX3

PTX3 Target Infomation Overview

Target Symbol: PTX3, pentraxin 3
Gene Groups: Long pentraxins
Alias: TSG-14
Previous Names: TNFAIP5
Alias Names: long pentraxin 3; pentaxin-related gene, rapidly induced by IL-1 beta; tumor necrosis factor, alpha-induced protein 5; pentraxin-related gene, rapidly induced by IL-1 beta; pentraxin 3, long

PTX3, pentraxin 3 Target Infomation by Species

Human
Mouse
Rat

Human PTX3 Target Information

Target Symbol: PTX3, pentraxin 3
Alias:
- long pentraxin 3
- pentaxin-related gene, rapidly induced by il-1 beta
- pentaxin-related gene, rapidly induced by IL-1 beta, tumor necrosis factor, alpha-induced protein 5
- pentaxin-related protein PTX3
- pentraxin 3, long
- pentraxin-3
- pentraxin-related gene, rapidly induced by IL-1 beta
- pentraxin-related protein PTX3
- TNF alpha-induced protein 5
- TNFAIP5
- TSG-14
- tumor necrosis factor alpha-induced protein 5
- tumor necrosis factor-inducible gene 14 protein
- tumor necrosis factor-inducible protein TSG-14
- tumor necrosis factor, alpha-induced protein 5
NCBI_Gene: 5806
UniProtKB: P26022

Human PTX3 Predicted Functions

Enables complement component C1q complex binding activity; identical protein binding activity; and virion binding activity. Involved in innate immune response; modulation by host of viral process; and negative regulation of viral entry into host cell. Located in extracellular space. Implicated in cystic fibrosis and invasive aspergillosis. Biomarker of hepatitis C; invasive aspergillosis; pelvic inflammatory disease; pulmonary aspergilloma; and toxic shock syndrome.

Mouse Ptx3 Target Information

Target Symbol: Ptx3, pentraxin related gene
Alias:
- AI607804
- expressed sequence AI607804
- pentraxin 3
- TSG-14
NCBI_Gene: 19288
- UniprotKB: P48759

Mouse Ptx3 Predicted Functions

Enables (1->3)-beta-D-glucan binding activity. Acts upstream of or within several processes, including opsonization; ovarian cumulus expansion; and positive regulation of nitric oxide biosynthetic process. Located in extracellular matrix. Is expressed in cochlea; cochlea epithelium; inner ear vestibular component; organ of Corti; and stria vascularis. Human ortholog(s) of this gene implicated in cystic fibrosis and invasive aspergillosis. Orthologous to human PTX3 (pentraxin 3).

Rat Ptx3 Target Information

Target Symbol: Ptx3, pentraxin 3
Alias:
- LOC689388
- pentraxin 3, long
- pentraxin related
- pentraxin related gene
- pentraxin-related protein PTX3
- similar to Pentraxin-related protein PTX3 precursor (Pentaxin-related protein PTX3) (Tumor necrosis factor-inducible protein TSG-14)
NCBI_Gene: 689388
UniProtKB: D3ZT94

Rat Ptx3 Predicted Functions

Predicted to enable several functions, including (1->3)-beta-D-glucan binding activity; complement component C1q complex binding activity; and virion binding activity. Predicted to be involved in innate immune response; modulation by host of viral process; and negative regulation of viral entry into host cell. Predicted to act upstream of or within several processes, including opsonization; ovarian cumulus expansion; and positive regulation of nitric oxide biosynthetic process. Predicted to be located in extracellular matrix. Predicted to be active in extracellular space. Human ortholog(s) of this gene implicated in cystic fibrosis and invasive aspergillosis. Orthologous to human PTX3 (pentraxin 3).

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