Human TNF-β/Lymphotoxin-α ELISA Kit

$350.00$450.00

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TARGETSLTA
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity2.02 pg/mL
Range62.5 pg/mL – 4000 pg/mL
Assay Time3.5 h
Recovery87% – 113%
Average Recovery1.02
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity2.02 pg/mL
Range62.5 pg/mL – 4000 pg/mL
Assay Time3.5 h
Recovery87% – 113%
Average Recovery1.02
Intra Precision3.0% – 6.2%
Inter-Precision3.5% – 4.7%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TNF-β/Lymphotoxin-α monoclonal antibody
Human TNF-β/Lymphotoxin-α freeze-dried standard
TNF-β/Lymphotoxin-α detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human TNF-β antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, TNF-β present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of TNF-β in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

LTA

LTA Target Infomation Overview

  • Target Symbol: LTA, lymphotoxin alpha
  • Gene Groups: Tumor necrosis factor superfamily
  • Alias: TNFSF1; LT
  • Previous Names: TNFB
  • Alias Names: TNF superfamily member 1; lymphotoxin alpha (TNF superfamily, member 1)

LTA, lymphotoxin alpha Target Infomation by Species

HumanMouseRat

Human LTA Target Information

  • Target Symbol: LTA, lymphotoxin alpha
  • Alias:
    • CDF
    • cholinergic differentiation factor
    • D factor
    • DIA
    • differentiation inhibitory activity
    • differentiation stimulating factor
    • differentiation-inducing factor
    • differentiation-stimulating factor
    • hepatocyte-stimulating factor III
    • HILDA
    • human interleukin in DA cells
    • leukemia inhibitory factor
    • LIF, interleukin 6 family cytokine
    • melanoma-derived LPL inhibitor
    • MLPLI
  • NCBI_Gene: 4049
  • UniProtKB: P01374

Human LTA Predicted Functions

Enables cytokine activity; growth factor activity; and leukemia inhibitory factor receptor binding activity. Involved in several processes, including leukemia inhibitory factor signaling pathway; positive regulation of protein modification process; and regulation of epithelial cell differentiation involved in kidney development. Acts upstream of or within several processes, including positive regulation of MAPK cascade; positive regulation of macrophage differentiation; and positive regulation of protein phosphorylation. Located in cytosol.

Mouse Lta Target Information

Mouse Lta Predicted Functions

Predicted to enable tumor necrosis factor receptor binding activity. Acts upstream of or within several processes, including lymph node development; positive regulation of immune response; and positive regulation of interferon-gamma production. Predicted to be located in extracellular space. Is expressed in several structures, including extraembryonic component; gut; hemolymphoid system; liver; and reproductive system. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); glucose metabolism disease (multiple); leishmaniasis (multiple); and lung disease (multiple). Orthologous to human LTA (lymphotoxin alpha).

Rat Lta Target Information

  • Target Symbol: Lta, lymphotoxin alpha
  • Alias:
    • LOC103690381
    • LT-alpha
    • lymphotoxin A
    • Lymphotoxin alpha (formerly tumor necrosis factor beta)
    • Lymphotoxin alpha (formerly tumor necrosis factor, beta)
    • lymphotoxin alpha (TNF superfamily, member 1)
    • lymphotoxin-alpha
    • lymphotoxin-alpha-like
    • TNF-beta
    • Tnfb
    • tumor necrosis factor beta
    • tumor necrosis factor ligand superfamily member 1
  • NCBI_Gene: 25008
  • UniProtKB: Q06332

Rat Lta Predicted Functions

Predicted to enable cytokine activity and tumor necrosis factor receptor binding activity. Involved in several processes, including negative regulation of fibroblast proliferation; positive regulation of glial cell proliferation; and response to lipopolysaccharide. Located in extracellular space. Biomarker of cystitis. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); glucose metabolism disease (multiple); leishmaniasis (multiple); and lung disease (multiple). Orthologous to human LTA (lymphotoxin alpha).

LTA Target News

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