Mouse CCL11/Eotaxin ELISA Kit

$350.00$450.00

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TARGETSCCL11
SPECIESMouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity0.73 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery83% – 114%
Average Recovery0.97
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity0.73 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery83% – 114%
Average Recovery0.97
Intra Precision3.8% – 6.4%
Inter-Precision2.2% – 3.4%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CCL11/Eotaxin monoclonal antibody
Mouse CCL11/Eotaxin freeze-dried standard
CCL11/Eotaxin detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse Eotaxin antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Eotaxin present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Eotaxin in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

CCL11

CCL11 Target Infomation Overview

  • Target Symbol: CCL11, C-C motif chemokine ligand 11
  • Gene Groups: Chemokine ligands
  • Alias: eotaxin; MGC22554
  • Previous Names: SCYA11
  • Alias Names: eotaxin-1; small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin); chemokine (C-C motif) ligand 11

CCL11, C-C motif chemokine ligand 11 Target Infomation by Species

HumanMouseRat

Human CCL11 Target Information

  • Target Symbol: CCL11, C-C motif chemokine ligand 11
  • Alias:
    • C-C motif chemokine 11
    • chemokine (C-C motif) ligand 11
    • eosinophil chemotactic protein
    • eotaxin
    • eotaxin-1
    • MGC22554
    • SCYA11
    • small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)
    • small-inducible cytokine A11
  • NCBI_Gene: 6356
  • UniProtKB: P51671

Human CCL11 Predicted Functions

Enables CCR3 chemokine receptor binding activity; chemokine activity; and protein dimerization activity. Involved in several processes, including eosinophil chemotaxis; positive regulation of GTPase activity; and positive regulation of actin filament polymerization. Located in extracellular region. Implicated in asthma; human immunodeficiency virus infectious disease; and hyperglycemia. Biomarker of several diseases, including cystic fibrosis; hyperglycemia; lung disease (multiple); nephrotic syndrome type 1; and ureteral obstruction.

Mouse Ccl11 Target Information

Mouse Ccl11 Predicted Functions

Predicted to enable CCR3 chemokine receptor binding activity; chemokine activity; and protein dimerization activity. Involved in several processes, including modulation of age-related behavioral decline; negative regulation of neurogenesis; and positive regulation of angiogenesis. Acts upstream of or within branching involved in mammary gland duct morphogenesis and mammary duct terminal end bud growth. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Is expressed in embryo; endocardial lining; liver parenchyma; liver vasculature; and yolk sac blood vessel. Human ortholog(s) of this gene implicated in asthma; human immunodeficiency virus infectious disease; hyperglycemia; and multiple sclerosis. Orthologous to several human genes including CCL11 (C-C motif chemokine ligand 11).

Rat Ccl11 Target Information

  • Target Symbol: Ccl11, C-C motif chemokine ligand 11
  • Alias:
    • C-C motif chemokine 11
    • chemokine (C-C motif) ligand 11
    • eosinophil chemotactic protein
    • eotaxin
    • Scya11
    • small inducible cytokine A11
    • small inducible cytokine subfamily A11
    • small-inducible cytokine A11
  • NCBI_Gene: 29397
  • UniProtKB: P97545

Rat Ccl11 Predicted Functions

Enables chemokine activity. Involved in several processes, including ERK1 and ERK2 cascade; leukocyte chemotaxis; and response to interleukin-13. Located in extracellular space. Used to study allergic rhinitis; asthma; pleurisy; and pneumonia. Biomarker of several diseases, including allergic conjunctivitis; pneumonia; prostatitis; rhinitis; and type 1 diabetes mellitus. Human ortholog(s) of this gene implicated in asthma; human immunodeficiency virus infectious disease; hyperglycemia; and multiple sclerosis. Orthologous to several human genes including CCL11 (C-C motif chemokine ligand 11).

CCL11 Target News

    5.4
    Effects of total alkaloids from Alstonia scholaris (L.) R. Br. on ovalbumin-induced asthma mice
    JOURNAL OF ETHNOPHARMACOLOGY
    3.2
    Plumbagin alleviates obesity-related asthma: Targeting inflammation, oxidative stress, and the AMPK pathway
    Immunity Inflammation and Disease
    Allergic Reaction
    Lung Disease (Asthma & COPD)

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