EK108EGA
EasyGo!™ Human IL-8 One-Step ELISA Kit
$294.00 – $420.00
Related Target | |
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Species | human |
Sample Type | Serum, plasma |
Sample Volume | Serum, plasma:10μL |
Sensitivity | 3.85 pg/mL |
Array Range | 15.625 pg/mL - 1000 pg/mL |
Assay Time | 1.5h |
Recovery | 85% - 122% |
Average Recovery | 102% |
ELISA Kit Detail Information
Related Target | |
---|---|
Species | human |
Sample Type | Serum, plasma |
Sample Volume | Serum, plasma:10μL |
Sensitivity | 3.85 pg/mL |
Array Range | 15.625 pg/mL - 1000 pg/mL |
Assay Time | 1.5h |
Recovery | 85% - 122% |
Average Recovery | 102% |
Intra Precision | 6.1% - 9.5% |
Inter Precision | 8.9% - 9.8% |
Plate | Detachable 96-well plate |
Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard should be stored at -20℃, while the other components should be stored at 4℃. |
Delivery | 4℃ blue ice transportation |
Components | 1× Washing Buffer 1× Assay Buffer Stop Solution Substrate TMB Standard Microplate Wells |
Assay Principle | This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human IL-8 has been immobilized onto microwells, and two pellets of the biotin-linked detect antibody specific for IL-8 (light yellow) and streptavidin-HRP (purple) are pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IL-8 present is bound by the immobilized antibody and detect antibody, of which the latter is conjugated with streptavidin-HRP in the incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-8 bound by the immobilized antibody. The color development is stopped and the intensity of the color is measured by microplate reader. |
Related Targets
CXCL8
No description available.
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