EK108EGA

EasyGo!™ Human IL-8 One-Step ELISA Kit

$294.00$420.00

Related Target
Specieshuman
Sample TypeSerum, plasma
Sample VolumeSerum, plasma:10μL
Sensitivity3.85 pg/mL
Array Range15.625 pg/mL - 1000 pg/mL
Assay Time1.5h
Recovery85% - 122%
Average Recovery102%

ELISA Kit Detail Information

Related Target
Specieshuman
Sample TypeSerum, plasma
Sample VolumeSerum, plasma:10μL
Sensitivity3.85 pg/mL
Array Range15.625 pg/mL - 1000 pg/mL
Assay Time1.5h
Recovery85% - 122%
Average Recovery102%
Intra Precision6.1% - 9.5%
Inter Precision8.9% - 9.8%
PlateDetachable 96-well plate
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components1× Washing Buffer
1× Assay Buffer
Stop Solution
Substrate TMB
Standard
Microplate Wells
Assay PrincipleThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human IL-8 has been immobilized onto microwells, and two pellets of the biotin-linked detect antibody specific for IL-8 (light yellow) and streptavidin-HRP (purple) are pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IL-8 present is bound by the immobilized antibody and detect antibody, of which the latter is conjugated with streptavidin-HRP in the incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-8 bound by the immobilized antibody. The color development is stopped and the intensity of the color is measured by microplate reader.

Related Targets

CXCL8

No description available.

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