Product Category
Newest Products
Glucagon ELISA Kit
$350.00 – $450.00
| TARGETS | GCG |
|---|---|
| SPECIES | Human Mouse Rat |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 100 μL |
| Sensitivity | 8.42 pg/mL |
| Range | 78.13 pg/mL – 5000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 80% – 127% |
| Average Recovery | 1.02 |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 100 μL |
| Sensitivity | 8.42 pg/mL |
| Range | 78.13 pg/mL – 5000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 80% – 127% |
| Average Recovery | 1.02 |
| Intra Precision | 1.5% – 3.7% |
| Inter-Precision | 4.4% – 6.2% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Glucagon monoclonal antibody Glucagon freeze-dried standard Glucagon detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-Glucagon antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the Glucagon present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Glucagon in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
GCG
GCG Target Infomation Overview
- Target Symbol: GCG, glucagon
- Gene Groups: Receptor ligands
- Alias: GLP1; GLP2; GRPP; GLP-1
GCG, glucagon Target Infomation by Species
HumanMouseRat
Human GCG Target Information
- Target Symbol: GCG, glucagon
- Alias:
- glicentin-related polypeptide
- GLP-1
- GLP1
- GLP2
- glucagon-like peptide 1
- glucagon-like peptide 2
- GRPP
- preproglucagon
- pro-glucagon
- NCBI_Gene: 2641
- UniProtKB: P01275
Human GCG Predicted Functions
Enables gastric inhibitory polypeptide receptor binding activity and identical protein binding activity. Predicted to be involved in several processes, including positive regulation of bicellular tight junction assembly; positive regulation of insulin secretion involved in cellular response to glucose stimulus; and protein kinase A signaling. Predicted to act upstream of or within negative regulation of execution phase of apoptosis; positive regulation of gluconeogenesis; and positive regulation of histone H3-K4 methylation. Predicted to be located in cytoplasm. Predicted to be active in extracellular space. Implicated in Alzheimer’s disease and prediabetes syndrome.
Mouse Gcg Target Information
- Target Symbol: Gcg, glucagon
- Alias:
- GLP-1
- Glu
- glucagon-like peptide I
- PPG
- NCBI_Gene: 14526
Mouse Gcg Predicted Functions
Predicted to enable identical protein binding activity and signaling receptor binding activity. Involved in several processes, including positive regulation of protein phosphorylation; positive regulation of transport; and regulation of intracellular signal transduction. Acts upstream of or within several processes, including positive regulation of gluconeogenesis; positive regulation of histone H3-K4 methylation; and regulation of insulin secretion. Located in cytoplasm and extracellular space. Is expressed in several structures, including brain; gut; and urogenital ridge. Human ortholog(s) of this gene implicated in Alzheimer’s disease and prediabetes syndrome. Orthologous to human GCG (glucagon).
Rat Gcg Target Information
- Target Symbol: Gcg, glucagon
- Alias:
- GLP-1
- Glp1
- glucagon-like peptide-1
- pro-glucagon
- NCBI_Gene: 24952
Rat Gcg Predicted Functions
Enables glucagon receptor binding activity and hormone activity. Involved in adenylate cyclase-modulating G protein-coupled receptor signaling pathway; negative regulation of appetite; and response to L-arginine. Located in extracellular space. Human ortholog(s) of this gene implicated in Alzheimer’s disease and prediabetes syndrome. Orthologous to human GCG (glucagon).
GCG Target News
Data not found.
