Human Galectin-3 ELISA Kit

$350.00$450.00

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TARGETSLGALS3
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity2.46 pg/mL
Range62.5 pg/mL – 4000 pg/mL
Assay Time3.5 h
Recovery85% – 105%
Average Recovery0.91
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity2.46 pg/mL
Range62.5 pg/mL – 4000 pg/mL
Assay Time3.5 h
Recovery85% – 105%
Average Recovery0.91
Intra Precision1.7% – 3.5%
Inter-Precision3.9% – 4.9%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Galectin-3A monoclonal antibody
Human Galectin-3A freeze-dried standard
Galectin-3A detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Galectin-3 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Galectin-3 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Galectin-3 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

LGALS3

LGALS3 Target Infomation Overview

  • Target Symbol: LGALS3, galectin 3
  • Gene Groups: Receptor ligands; Galectins
  • Alias: MAC-2; GALIG
  • Previous Names: LGALS2
  • Alias Names: advanced glycation end-product receptor 3; lectin, galactoside-binding, soluble, 3

LGALS3, galectin 3 Target Infomation by Species

HumanMouseRat

Human LGALS3 Target Information

  • Target Symbol: LGALS3, galectin 3
  • Alias:
    • 35 kDa lectin
    • advanced glycation end-product receptor 3
    • carbohydrate-binding protein 35
    • CBP35
    • GAL3
    • galactose-specific lectin 3
    • GALBP
    • galectin-3
    • GALIG
    • IgE-binding protein
    • L31
    • laminin-binding protein
    • lectin L-29
    • lectin, galactose binding, soluble 3
    • lectin, galactoside binding soluble 3
    • lectin, galactoside-binding, soluble, 3
    • LGALS2
    • MAC-2 antigen
    • MAC2
  • NCBI_Gene: 3958
  • UniProtKB: P17931

Human LGALS3 Predicted Functions

Enables several functions, including IgE binding activity; chemoattractant activity; and laminin binding activity. Involved in several processes, including leukocyte chemotaxis; positive regulation of protein localization to plasma membrane; and regulation of apoptotic process. Located in immunological synapse; mitochondrial inner membrane; and nucleus. Is active in extracellular space. Colocalizes with collagen-containing extracellular matrix. Implicated in asthma. Biomarker of aortic valve stenosis and liver cirrhosis.

Mouse Lgals3 Target Information

Mouse Lgals3 Predicted Functions

Predicted to enable several functions, including IgE binding activity; advanced glycation end-product receptor activity; and signaling receptor binding activity. Involved in negative regulation of T cell receptor signaling pathway; negative regulation of endocytosis; and negative regulation of lymphocyte activation. Acts upstream of or within extracellular matrix organization and skeletal system development. Located in several cellular components, including external side of plasma membrane; glial cell projection; and immunological synapse. Is expressed in several structures, including alimentary system; genitourinary system; respiratory system; skeleton; and skin. Used to study fatty liver disease. Human ortholog(s) of this gene implicated in asthma. Orthologous to human LGALS3 (galectin 3).

Rat Lgals3 Target Information

Rat Lgals3 Predicted Functions

Enables several functions, including Fc-gamma receptor I complex binding activity; IgE binding activity; and advanced glycation end-product receptor activity. Involved in several processes, including negative regulation of cell proliferation in bone marrow; positive regulation of angiogenesis; and positive regulation of serotonin secretion. Located in several cellular components, including cell surface; extracellular space; and nucleus. Biomarker of several diseases, including acute kidney failure; attention deficit hyperactivity disorder; brain ischemia; congestive heart failure; and type 1 diabetes mellitus. Human ortholog(s) of this gene implicated in asthma. Orthologous to human LGALS3 (galectin 3).

LGALS3 Target News

    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Tumor Marker

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