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Human Galectin-3 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 100 μL |
| Sensitivity | 2.46 pg/mL |
| Range | 62.5 pg/mL – 4000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 85% – 105% |
| Average Recovery | 0.91 |
| Intra Precision | 1.7% – 3.5% |
| Inter-Precision | 3.9% – 4.9% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Galectin-3A monoclonal antibody Human Galectin-3A freeze-dried standard Galectin-3A detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Galectin-3 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Galectin-3 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Galectin-3 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
LGALS3
LGALS3 Target Infomation Overview
- Target Symbol: LGALS3, galectin 3
- Gene Groups: Receptor ligands; Galectins
- Alias: MAC-2; GALIG
- Previous Names: LGALS2
- Alias Names: advanced glycation end-product receptor 3; lectin, galactoside-binding, soluble, 3
LGALS3, galectin 3 Target Infomation by Species
HumanMouseRat
Human LGALS3 Target Information
- Target Symbol: LGALS3, galectin 3
- Alias:
- 35 kDa lectin
- advanced glycation end-product receptor 3
- carbohydrate-binding protein 35
- CBP35
- GAL3
- galactose-specific lectin 3
- GALBP
- galectin-3
- GALIG
- IgE-binding protein
- L31
- laminin-binding protein
- lectin L-29
- lectin, galactose binding, soluble 3
- lectin, galactoside binding soluble 3
- lectin, galactoside-binding, soluble, 3
- LGALS2
- MAC-2 antigen
- MAC2
- NCBI_Gene: 3958
- UniProtKB: P17931
Human LGALS3 Predicted Functions
Enables several functions, including IgE binding activity; chemoattractant activity; and laminin binding activity. Involved in several processes, including leukocyte chemotaxis; positive regulation of protein localization to plasma membrane; and regulation of apoptotic process. Located in immunological synapse; mitochondrial inner membrane; and nucleus. Is active in extracellular space. Colocalizes with collagen-containing extracellular matrix. Implicated in asthma. Biomarker of aortic valve stenosis and liver cirrhosis.
Mouse Lgals3 Target Information
- Target Symbol: Lgals3, lectin, galactose binding, soluble 3
- Alias:
- gal3
- galectin-3
- L-34
- Mac-2
- NCBI_Gene: 16854
Mouse Lgals3 Predicted Functions
Predicted to enable several functions, including IgE binding activity; advanced glycation end-product receptor activity; and signaling receptor binding activity. Involved in negative regulation of T cell receptor signaling pathway; negative regulation of endocytosis; and negative regulation of lymphocyte activation. Acts upstream of or within extracellular matrix organization and skeletal system development. Located in several cellular components, including external side of plasma membrane; glial cell projection; and immunological synapse. Is expressed in several structures, including alimentary system; genitourinary system; respiratory system; skeleton; and skin. Used to study fatty liver disease. Human ortholog(s) of this gene implicated in asthma. Orthologous to human LGALS3 (galectin 3).
Rat Lgals3 Target Information
- Target Symbol: Lgals3, galectin 3
- Alias:
- 35 kDa lectin
- AGE-R3
- carbohydrate-binding protein 35
- CBP 35
- CBP30
- epsilon BP
- gal-3
- galactose-specific lectin 3
- galectin-3
- IgE binding protein
- L-34
- laminin-binding protein
- lectin galactoside-binding soluble 3 protein
- lectin L-29
- lectin, galactose binding, soluble 3
- lectin, galactoside-binding, soluble, 3
- mac-2 antigen
- MGC105387
- NCBI_Gene: 83781
Rat Lgals3 Predicted Functions
Enables several functions, including Fc-gamma receptor I complex binding activity; IgE binding activity; and advanced glycation end-product receptor activity. Involved in several processes, including negative regulation of cell proliferation in bone marrow; positive regulation of angiogenesis; and positive regulation of serotonin secretion. Located in several cellular components, including cell surface; extracellular space; and nucleus. Biomarker of several diseases, including acute kidney failure; attention deficit hyperactivity disorder; brain ischemia; congestive heart failure; and type 1 diabetes mellitus. Human ortholog(s) of this gene implicated in asthma. Orthologous to human LGALS3 (galectin 3).
