Name: Human/Mouse/Rat TGF-β2 ELISA Kit
SKU: EK9162
Availability: InStock

EK9162

Human/Mouse/Rat TGF-β2 ELISA Kit

$245.00 – $350.00

Related Target	TGFB2
Species	human,mouse,rat
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	0.33 pg/mL
Array Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	88% – 120%
Average Recovery	107%

SKU: EK9162 Category: ELISA Kit

ELISA Kit Detail Information

Related Target	TGFB2
Species	human,mouse,rat
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	0.33 pg/mL
Array Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	88% – 120%
Average Recovery	107%
Intra Precision	2.7% – 4.5%
Inter Precision	3.3% – 4.8%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TGF-β2 monoclonal antibody Human /Mouse /Rat TGF-β2 freeze-dried standard TGF-β2 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human /mouse /rat TGF-β2 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, TGF-β2 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of TGF-β2 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

TGFB2

TGFB2 Target Infomation Overview

Target Symbol: TGFB2, transforming growth factor beta 2
Gene Groups: Transforming growth factor beta family
Alias Names: prepro-transforming growth factor beta-2; transforming growth factor, beta 2

TGFB2, transforming growth factor beta 2 Target Infomation by Species

Human
Mouse
Rat

Human TGFB2 Target Information

Target Symbol: TGFB2, transforming growth factor beta 2
Alias:
- BSC-1 cell growth inhibitor
- cetermin
- G-TSF
- glioblastoma-derived T-cell suppressor factor
- LDS4
- MGC116892
- polyergin
- prepro-transforming growth factor beta-2
- TGF-beta-2
- TGF-beta2
- transforming growth factor beta-2
- transforming growth factor beta-2 proprotein
- transforming growth factor, beta 2
NCBI_Gene: 7042
UniProtKB: P61812

Human TGFB2 Predicted Functions

Enables amyloid-beta binding activity; protein homodimerization activity; and transforming growth factor beta receptor binding activity. Involved in several processes, including animal organ development; cell surface receptor signaling pathway; and positive regulation of signal transduction. Located in collagen-containing extracellular matrix and extracellular space. Implicated in Loeys-Dietz syndrome 4 and colorectal cancer. Biomarker of colon carcinoma; colorectal cancer; and liver cirrhosis.

Mouse Tgfb2 Target Information

Target Symbol: Tgfb2, transforming growth factor, beta 2
Alias:
- BB105277
- expressed sequence BB105277
- Tgf-beta2
- Tgfb-2
NCBI_Gene: 21808

Mouse Tgfb2 Predicted Functions

Predicted to enable several functions, including amyloid-beta binding activity; protein homodimerization activity; and transforming growth factor beta receptor binding activity. Involved in several processes, including animal organ development; regulation of epithelial to mesenchymal transition involved in endocardial cushion formation; and tube morphogenesis. Acts upstream of or within several processes, including animal organ development; neuron differentiation; and positive regulation of apoptotic process. Located in several cellular components, including axon; endosome; and neuronal cell body. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; heart; and sensory organ. Used to study Loeys-Dietz syndrome. Human ortholog(s) of this gene implicated in Loeys-Dietz syndrome 4 and colorectal cancer. Orthologous to human TGFB2 (transforming growth factor beta 2).

Rat Tgfb2 Target Information

Target Symbol: Tgfb2, transforming growth factor, beta 2
Alias:
- TGF beta 2 protein
- TGF-B2
- TGF-beta-2
- transforming growth factor beta-2
- transforming growth factor beta-2 proprotein
NCBI_Gene: 81809

Rat Tgfb2 Predicted Functions

Enables identical protein binding activity and protein N-terminus binding activity. Involved in several processes, including animal organ development; negative regulation of release of sequestered calcium ion into cytosol; and regulation of apoptotic process. Located in several cellular components, including basement membrane; secretory granule; and trans-Golgi network. Biomarker of diabetic neuropathy; myocardial infarction; and osteochondrodysplasia. Human ortholog(s) of this gene implicated in Loeys-Dietz syndrome 4 and colorectal cancer. Orthologous to human TGFB2 (transforming growth factor beta 2).

Citations for Human/Mouse/Rat TGF-β2 ELISA Kit

There are no citations for this product yet.

If you buy this product and mention it in your thesis, we welcome you to provide us with the thesis information. As a thank you, we will give you some gifts, such as discount coupons.

Citations data is updating...

Competitive ELISA: Principles, Methods, and Key Differences

Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.

ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol

Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.

ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects

Learn whether to use serum or plasma for ELISA testing, including preparation protocols, key differences (fibrinogen, clotting factors), and how hemolysis impacts results. Essential guide for accurate immunoassays.