Multi Sciences Biotech Shines at AACR 2025: Showcasing China’s Biotech Innovation on the Global Stage
From April 25-30, 2025, Lianke Bio made a remarkable appearance at the American Association for Cancer Research (AACR) Annual Meeting in Chicago—the world’s premier oncology event. Led by General Manager Grace Song, the team bridged Sino-US trade barriers to present cutting-edge ELISA kits, flow antibodies, and detection kits, captivating global researchers with their high-quality, cost-effective solutions.
1 - Essential ELISA Tips: Prevent Common Failures & Ensure Reliable Results
Essential ELISA Tips: Prevent Common Failures & Ensure Reliable Results Struggling with ELISA failures? Follow these expert-recommended best practices to optimize your assay performance: Key Recommendations for Successful ELISA Experiments:
- Pre-experiment preparation
- Reagent quality control
- Precision techniques
4 - ELISA Troubleshooting: Normal Standard Curve But Low/No Sample Signal?
Is your ELISA showing a proper standard curve but weak or undetectable sample signals? Discover common causes and proven solutions: Key Reasons & Fixes for Low Sample Detection:
- Degraded/improperly prepared samples
- Low target concentration
- Excessive freeze-thaw cycles
- Matrix interference
- Insufficient incubation time
5 - High Background in ELISA? Causes and Solutions for Optimal Results
Struggling with high background values in ELISA, leading to poor signal-to-noise ratios? Discover the key causes and expert-recommended fixes:
- Inadequate washing
- Non-specific binding
- Contaminated reagents
- Overly concentrated antibodies
- Plate overdevelopment
- Improper storage of substrates
6 - Why ELISA CV Values Are High & How to Fix Poor Repeatability
A high coefficient of variation (CV) in ELISA indicates poor repeatability, often caused by technical errors. Common issues and solutions include:
- Air bubbles in wells
- Pipetting inaccuracies
- Edge effect (temperature variation)
- Inconsistent reagent batches
- Poor microsphere dissolution (one-step ELISA)
- Microsphere moisture exposure
7 - Sandwich ELISA & Competitive ELISA Kit Data Analysis Guide
Learn how to accurately analyze ELISA kit data using the Double-Antibody Sandwich and Competitive Assay methods. Key steps include:
- Optical Density (OD) Measurement
- Standard Curve Generation
- Sample Concentration Calculation
- Competitive ELISA Analysis
Competitive ELISA: Principles, Methods, and Key Differences
Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.