6 - Why ELISA CV Values Are High & How to Fix Poor Repeatability

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ELISA IS POEM

Possible Causes & Recommended Actions

Air Bubbles in the Microplate

Air Bubbles in the Microplate

Ensure that there are no air bubbles in the microplate during incubation and reading

Low Accuracy of Pipette

Low Accuracy of Pipette

Regularly calibrate the pipette and select the correct pipetting method

Edge Effect

Edge Effect

Ensure that the microplate and all reagents are at the same temperature

Reagent Mixing

Reagent Mixing

Do not mix reagents from different batches or different indicators for the same indicator

Insufficient Dissolution of Microspheres in One-Step Experiments

Insufficient Dissolution of Microspheres in One-Step Experiments

Ensure sufficient dissolution of microspheres in each well of the one-step experiments and control the time interval for sample loading in each well as much as possible

Moisture Dissolution of Microspheres in One-Step Experiments

Moisture Dissolution of Microspheres in One-Step Experiments

Avoid exposing microspheres to high humidity environments

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