3 - ELISA Standard Curve Problems? Fix Poor Linearity & Gradient Issues

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ELISA IS POEM

Possible Causes & Recommended Actions

Incorrect Incubation Temperature or Time

Incorrect Incubation Temperature or Time

Incubate according to the manuals.

Insufficient Washing or Incomplete Removal of Residual Liquids During Plate Washing

Insufficient Washing or Incomplete Removal of Residual Liquids During Plate Washing

Ensure no residues in the wells during plate tapping and wash according to the manuals

Low Accuracy of Pipette

Low Accuracy of Pipette

Regularly calibrate the pipette and select the correct pipetting method

Insufficient Dissolution of Microspheres in One-Step Experiments

Insufficient Dissolution of Microspheres in One-Step Experiments

Ensure sufficient dissolution of microspheres in each well of the one-step experiments and control the time interval for sample loading in each well as much as possible

Moisture Dissolution of Microspheres in One-Step Experiments

Moisture Dissolution of Microspheres in One-Step Experiments

Avoid exposing microspheres to high humidity environments

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