5 - High Background in ELISA? Causes and Solutions for Optimal Results

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ELISA IS POEM

Possible Causes & Recommended Actions

The Incubation Temperature is Too High

The Incubation Temperature is Too High

Follow the instructions for operation. The ELISA kits from Linkage Biosciences are incubated at room temperature (25°C±3°C). The one-step ELISA kits are incubated at room temperature/37°C.

Using Hemolytic Samples

Using Hemolytic Samples

Using non hemolytic samples

There are Residues or Incomplete Washing Observed in the Wells During the Washing Step

There are Residues or Incomplete Washing Observed in the Wells During the Washing Step

Washing according to the manuals

Something Wrong with the Water Quality Used for Preparing the Solution

Something Wrong with the Water Quality Used for Preparing the Solution

Select clean and sterile ultrapure water

Insufficient Dissolution of Microspheres in One-Step Experiments

Insufficient Dissolution of Microspheres in One-Step Experiments

Ensure sufficient dissolution of microspheres in each well of the one-step experiments and control the time interval for sample loading in each well as much as possible

Moisture Dissolution of Microspheres in One-Step Experiments

Moisture Dissolution of Microspheres in One-Step Experiments

Avoid exposing microspheres to high humidity environments

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