4 - ELISA Troubleshooting: Normal Standard Curve But Low/No Sample Signal?

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ELISA IS POEM

Possible Causes & Recommended Actions

The Sample is Not Fresh or the Preparation Method is Inappropriate

The Sample is Not Fresh or the Preparation Method is Inappropriate

Reprepare the sample and use a suitable preparation method

Low Expression Level of the Target Indicator in the Sample

Low Expression Level of the Target Indicator in the Sample

Increase the amount of sample loading

The Sample Has Been Stored for Too Long or Subjected to Multiple Freeze-thaw Cycles

The Sample Has Been Stored for Too Long or Subjected to Multiple Freeze-thaw Cycles

It is recommended to use freshly prepared samples and store aliquots for no longer than 3 months to avoid multiple freeze-thaw cycles

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