Human Granulin/GRN ELISA Kit

$350.00$450.00

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TARGETSGRN
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity10.81 pg/mL
Range125 pg/mL – 8000 pg/mL
Assay Time3.5 h
Recovery92% – 135%
Average Recovery1.04
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity10.81 pg/mL
Range125 pg/mL – 8000 pg/mL
Assay Time3.5 h
Recovery92% – 135%
Average Recovery1.04
Intra Precision3.3% – 4.3%
Inter-Precision3.9% – 4.8%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Granulin/GRN monoclonal antibody
Human Granulin/GRN freeze-dried standard
Granulin/GRN detect Antibody
Standard Diluent
HRP-labeled streptavidin
Signal enhancer concentrate
Signal enhancer diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human GRN antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, GRN present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of GRN in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

GRN

GRN Target Infomation Overview

  • Target Symbol: GRN, granulin precursor
  • Alias: PCDGF; PGRN; CLN11
  • Alias Names: progranulin

GRN, granulin precursor Target Infomation by Species

HumanMouseRat

Human GRN Target Information

  • Target Symbol: GRN, granulin precursor
  • Alias:
    • acrogranin
    • CLN11
    • epithelin
    • GEP
    • glycoprotein 88
    • glycoprotein of 88 Kda
    • GP88
    • granulin
    • granulin-epithelin
    • granulins
    • PC cell-derived growth factor
    • PCDGF
    • PEPI
    • PGRN
    • proepithelin
    • progranulin
  • NCBI_Gene: 2896
  • UniProtKB: P28799

Human GRN Predicted Functions

Enables chaperone binding activity. Involved in several processes, including lysosomal lumen acidification; negative regulation of respiratory burst involved in inflammatory response; and positive regulation of lysosome organization. Located in several cellular components, including Golgi apparatus; late endosome; and lysosomal membrane. Implicated in dementia (multiple); neurodegenerative disease (multiple); neuronal ceroid lipofuscinosis 11; and primary progressive multiple sclerosis. Biomarker of several diseases, including Grn-related frontotemporal lobar degeneration with Tdp43 inclusions; atherosclerosis; autistic disorder; multiple sclerosis (multiple); and neurodegenerative disease (multiple).

Mouse Grn Target Information

Mouse Grn Predicted Functions

Predicted to enable chaperone binding activity. Involved in several processes, including astrocyte activation involved in immune response; positive regulation of cellular component organization; and regulation of defense response. Acts upstream of or within several processes, including blastocyst hatching; locomotory exploration behavior; and lysosomal protein catabolic process. Located in several cellular components, including endoplasmic reticulum; lysosome; and trans-Golgi network. Is expressed in several structures, including early conceptus; gonad; integument; nervous system; and placenta. Used to study Grn-related frontotemporal lobar degeneration with Tdp43 inclusions and nephrogenic diabetes insipidus. Human ortholog(s) of this gene implicated in dementia (multiple); neurodegenerative disease (multiple); neuronal ceroid lipofuscinosis 11; and primary progressive multiple sclerosis. Orthologous to human GRN (granulin precursor).

Rat Grn Target Information

Rat Grn Predicted Functions

Predicted to enable chaperone binding activity. Involved in several processes, including chondrocyte proliferation; positive regulation of dendritic spine development; and response to estradiol. Predicted to be located in several cellular components, including late endosome; lysosomal membrane; and trans-Golgi network. Predicted to be active in extracellular region. Biomarker of status epilepticus. Human ortholog(s) of this gene implicated in dementia (multiple); neurodegenerative disease (multiple); neuronal ceroid lipofuscinosis 11; and primary progressive multiple sclerosis. Orthologous to human GRN (granulin precursor).

GRN Target News

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