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Human Ki-67/MKI67 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 50 μL |
| Sensitivity | 0.35 pg/mL |
| Range | 6.25 pg/mL – 400 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 85% – 102% |
| Average Recovery | 0.95 |
| Intra Precision | 1.9% – 2.5% |
| Inter-Precision | 2.4%-3.0% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Ki-67/MKI67 monoclonal antibody Human Ki-67/MKI67 freeze-dried standard Human Ki-67/MKI67 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Ki-67 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Ki-67 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Ki-67 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
MKI67
MKI67 Target Infomation Overview
- Target Symbol: MKI67, marker of proliferation Ki-67
- Gene Groups: Protein phosphatase 1 regulatory subunits
- Alias: MIB-1; PPP1R105; Ki-67
- Alias Names: protein phosphatase 1, regulatory subunit 105; Molecular Immunology Borstel antibody 1; antigen identified by monoclonal antibody Ki-67
MKI67, marker of proliferation Ki-67 Target Infomation by Species
HumanMouseRat
Human MKI67 Target Information
- Target Symbol: MKI67, marker of proliferation Ki-67
- Alias:
- antigen identified by monoclonal antibody Ki-67
- antigen KI-67
- antigen Ki67
- KIA
- MIB-
- MIB-1
- Molecular Immunology Borstel antibody 1
- PPP1R105
- proliferation marker protein Ki-67
- proliferation-related Ki-67 antigen
- NCBI_Gene: 4288
- UniProtKB: P46013
Human MKI67 Predicted Functions
Enables protein C-terminus binding activity. Involved in regulation of chromosome segregation and regulation of mitotic nuclear division. Located in chromosome; nuclear body; and nucleolus. Colocalizes with condensed chromosome. Implicated in Crohn’s disease; breast cancer; human immunodeficiency virus infectious disease; and pancreatic cancer. Biomarker of several diseases, including Barrett’s esophagus; autoimmune disease of musculoskeletal system (multiple); endocrine gland cancer (multiple); gastrointestinal system cancer (multiple); and interstitial cystitis.
Mouse Mki67 Target Information
- Target Symbol: Mki67, antigen identified by monoclonal antibody Ki 67
- Alias:
- D630048A14Rik
- Ki-67
- Ki67
- RIKEN cDNA D630048A14 gene
- NCBI_Gene: 17345
Mouse Mki67 Predicted Functions
Predicted to enable protein C-terminus binding activity. Involved in regulation of chromatin organization. Acts upstream of or within cholangiocyte proliferation; hepatocyte proliferation; and meiotic cell cycle. Located in chromosome; cytoplasm; and nucleolus. Is expressed in several structures, including alimentary system; brain; genitourinary system; sensory organ; and skin. Human ortholog(s) of this gene implicated in Crohn’s disease; breast cancer; human immunodeficiency virus infectious disease; and pancreatic cancer. Orthologous to human MKI67 (marker of proliferation Ki-67).
Rat Mki67 Target Information
- Target Symbol: Mki67, marker of proliferation Ki-67
- Alias:
- antigen identified by monoclonal antibody Ki-67
- antigen KI-67
- LOC291234
- proliferation marker protein Ki-67
- NCBI_Gene: 291234
- UniProtKB: D4A0Y6
Rat Mki67 Predicted Functions
Predicted to enable protein C-terminus binding activity. Involved in several processes, including DNA metabolic process; animal organ regeneration; and cellular response to heat. Located in nucleus. Used to study high grade glioma; leiomyoma; and prostate carcinoma. Biomarker of colon cancer; kidney disease; mucositis; type 2 diabetes mellitus; and urinary bladder cancer. Human ortholog(s) of this gene implicated in Crohn’s disease; breast cancer; human immunodeficiency virus infectious disease; and pancreatic cancer. Orthologous to human MKI67 (marker of proliferation Ki-67).
