Human Ki-67/MKI67 ELISA Kit

$350.00$450.00

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TARGETSMKI67
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity0.35 pg/mL
Range6.25 pg/mL – 400 pg/mL
Assay Time3.5 h
Recovery85% – 102%
Average Recovery0.95
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity0.35 pg/mL
Range6.25 pg/mL – 400 pg/mL
Assay Time3.5 h
Recovery85% – 102%
Average Recovery0.95
Intra Precision1.9% – 2.5%
Inter-Precision2.4%-3.0%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Ki-67/MKI67 monoclonal antibody
Human Ki-67/MKI67 freeze-dried standard
Human Ki-67/MKI67 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Ki-67 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Ki-67 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Ki-67 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

MKI67

MKI67 Target Infomation Overview

  • Target Symbol: MKI67, marker of proliferation Ki-67
  • Gene Groups: Protein phosphatase 1 regulatory subunits
  • Alias: MIB-1; PPP1R105; Ki-67
  • Alias Names: protein phosphatase 1, regulatory subunit 105; Molecular Immunology Borstel antibody 1; antigen identified by monoclonal antibody Ki-67

MKI67, marker of proliferation Ki-67 Target Infomation by Species

HumanMouseRat

Human MKI67 Target Information

  • Target Symbol: MKI67, marker of proliferation Ki-67
  • Alias:
    • antigen identified by monoclonal antibody Ki-67
    • antigen KI-67
    • antigen Ki67
    • KIA
    • MIB-
    • MIB-1
    • Molecular Immunology Borstel antibody 1
    • PPP1R105
    • proliferation marker protein Ki-67
    • proliferation-related Ki-67 antigen
  • NCBI_Gene: 4288
  • UniProtKB: P46013

Human MKI67 Predicted Functions

Enables protein C-terminus binding activity. Involved in regulation of chromosome segregation and regulation of mitotic nuclear division. Located in chromosome; nuclear body; and nucleolus. Colocalizes with condensed chromosome. Implicated in Crohn’s disease; breast cancer; human immunodeficiency virus infectious disease; and pancreatic cancer. Biomarker of several diseases, including Barrett’s esophagus; autoimmune disease of musculoskeletal system (multiple); endocrine gland cancer (multiple); gastrointestinal system cancer (multiple); and interstitial cystitis.

Mouse Mki67 Target Information

Mouse Mki67 Predicted Functions

Predicted to enable protein C-terminus binding activity. Involved in regulation of chromatin organization. Acts upstream of or within cholangiocyte proliferation; hepatocyte proliferation; and meiotic cell cycle. Located in chromosome; cytoplasm; and nucleolus. Is expressed in several structures, including alimentary system; brain; genitourinary system; sensory organ; and skin. Human ortholog(s) of this gene implicated in Crohn’s disease; breast cancer; human immunodeficiency virus infectious disease; and pancreatic cancer. Orthologous to human MKI67 (marker of proliferation Ki-67).

Rat Mki67 Target Information

  • Target Symbol: Mki67, marker of proliferation Ki-67
  • Alias:
    • antigen identified by monoclonal antibody Ki-67
    • antigen KI-67
    • LOC291234
    • proliferation marker protein Ki-67
  • NCBI_Gene: 291234
  • UniProtKB: D4A0Y6

Rat Mki67 Predicted Functions

Predicted to enable protein C-terminus binding activity. Involved in several processes, including DNA metabolic process; animal organ regeneration; and cellular response to heat. Located in nucleus. Used to study high grade glioma; leiomyoma; and prostate carcinoma. Biomarker of colon cancer; kidney disease; mucositis; type 2 diabetes mellitus; and urinary bladder cancer. Human ortholog(s) of this gene implicated in Crohn’s disease; breast cancer; human immunodeficiency virus infectious disease; and pancreatic cancer. Orthologous to human MKI67 (marker of proliferation Ki-67).

MKI67 Target News

    Transplantation
    Tumor Marker

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