Human Ki-67/MKI67 ELISA Kit

$245.00 – $350.00

Related Target	MKI67
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL
Sensitivity	0.35 pg/mL
Array Range	6.25 pg/mL – 400 pg/mL
Assay Time	3.5 h
Recovery	85% – 102%
Average Recovery	95%

ELISA Kit Detail Information

Related Target	MKI67
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL
Sensitivity	0.35 pg/mL
Array Range	6.25 pg/mL – 400 pg/mL
Assay Time	3.5 h
Recovery	85% – 102%
Average Recovery	95%
Intra Precision	1.9% – 2.5%
Inter Precision	2.4% - 3.0%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Ki-67/MKI67 monoclonal antibody Human Ki-67/MKI67 freeze-dried standard Human Ki-67/MKI67 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Ki-67 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Ki-67 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Ki-67 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

MKI67

MKI67 Target Infomation Overview

Target Symbol: MKI67, marker of proliferation Ki-67
Gene Groups: Protein phosphatase 1 regulatory subunits
Alias: MIB-1; PPP1R105; Ki-67
Alias Names: protein phosphatase 1, regulatory subunit 105; Molecular Immunology Borstel antibody 1; antigen identified by monoclonal antibody Ki-67

MKI67, marker of proliferation Ki-67 Target Infomation by Species

Human
Mouse
Rat

Human MKI67 Target Information

Target Symbol: MKI67, marker of proliferation Ki-67
Alias:
- antigen identified by monoclonal antibody Ki-67
- antigen KI-67
- antigen Ki67
- KIA
- MIB-
- MIB-1
- Molecular Immunology Borstel antibody 1
- PPP1R105
- proliferation marker protein Ki-67
- proliferation-related Ki-67 antigen
NCBI_Gene: 4288
UniProtKB: P46013

Human MKI67 Predicted Functions

Enables protein C-terminus binding activity. Involved in regulation of chromosome segregation and regulation of mitotic nuclear division. Located in chromosome; nuclear body; and nucleolus. Colocalizes with condensed chromosome. Implicated in Crohn’s disease; breast cancer; human immunodeficiency virus infectious disease; and pancreatic cancer. Biomarker of several diseases, including Barrett’s esophagus; autoimmune disease of musculoskeletal system (multiple); endocrine gland cancer (multiple); gastrointestinal system cancer (multiple); and interstitial cystitis.

Mouse Mki67 Target Information

Target Symbol: Mki67, antigen identified by monoclonal antibody Ki 67
Alias:
- D630048A14Rik
- Ki-67
- Ki67
- RIKEN cDNA D630048A14 gene
NCBI_Gene: 17345
- UniprotKB: E9PVX6
- UniprotKB: A0A1B0GQV9

Mouse Mki67 Predicted Functions

Predicted to enable protein C-terminus binding activity. Involved in regulation of chromatin organization. Acts upstream of or within cholangiocyte proliferation; hepatocyte proliferation; and meiotic cell cycle. Located in chromosome; cytoplasm; and nucleolus. Is expressed in several structures, including alimentary system; brain; genitourinary system; sensory organ; and skin. Human ortholog(s) of this gene implicated in Crohn’s disease; breast cancer; human immunodeficiency virus infectious disease; and pancreatic cancer. Orthologous to human MKI67 (marker of proliferation Ki-67).

Rat Mki67 Target Information

Target Symbol: Mki67, marker of proliferation Ki-67
Alias:
- antigen identified by monoclonal antibody Ki-67
- antigen KI-67
- LOC291234
- proliferation marker protein Ki-67
NCBI_Gene: 291234
UniProtKB: D4A0Y6

Rat Mki67 Predicted Functions

Predicted to enable protein C-terminus binding activity. Involved in several processes, including DNA metabolic process; animal organ regeneration; and cellular response to heat. Located in nucleus. Used to study high grade glioma; leiomyoma; and prostate carcinoma. Biomarker of colon cancer; kidney disease; mucositis; type 2 diabetes mellitus; and urinary bladder cancer. Human ortholog(s) of this gene implicated in Crohn’s disease; breast cancer; human immunodeficiency virus infectious disease; and pancreatic cancer. Orthologous to human MKI67 (marker of proliferation Ki-67).

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