Human sCD40L ELISA Kit

$350.00$450.00

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TARGETSCD40LG
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity4.09 pg/mL
Range62.5 ng/mL – 4000 ng/mL
Assay Time3.5 h
Recovery88% – 110%
Average Recovery1.01
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity4.09 pg/mL
Range62.5 ng/mL – 4000 ng/mL
Assay Time3.5 h
Recovery88% – 110%
Average Recovery1.01
Intra Precision5.2% – 6.2%
Inter-Precision3.2% – 8.4%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-sCD40L monoclonal antibody
Human sCD40L freeze-dried standard
sCD40L detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human sCD40L antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, sCD40L present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of sCD40L in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

CD40LG

CD40LG Target Infomation Overview

  • Target Symbol: CD40LG, CD40 ligand
  • Gene Groups: CD molecules; Tumor necrosis factor superfamily
  • Alias: CD40L; TRAP; gp39; hCD40L; CD154
  • Previous Names: HIGM1; IMD3; TNFSF5
  • Alias Names: CD40 antigen ligand; tumor necrosis factor (ligand) superfamily member 5; T-B cell-activating molecule; TNF-related activation protein; hyper-IgM syndrome; tumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome)

CD40LG, CD40 ligand Target Infomation by Species

HumanMouseRat

Human CD40LG Target Information

  • Target Symbol: CD40LG, CD40 ligand
  • Alias:
    • CD154
    • CD40 antigen ligand
    • CD40-L
    • CD40L
    • gp39
    • hCD40L
    • HIGM1
    • IGM
    • IMD3
    • T-B cell-activating molecule
    • T-BAM
    • T-cell antigen Gp39
    • TNF-related activation protein
    • TNFSF5
    • TRAP
    • tumor necrosis factor (ligand) superfamily member 5
  • NCBI_Gene: 959
  • UniProtKB: P29965

Human CD40LG Predicted Functions

Enables CD40 receptor binding activity and integrin binding activity. Involved in several processes, including cell surface receptor signaling pathway; positive regulation of cytokine production; and regulation of apoptotic process. Located in cell surface. Implicated in several diseases, including Kawasaki disease; autoimmune disease (multiple); combined T cell and B cell immunodeficiency (multiple); hematologic cancer (multiple); and lupus nephritis. Biomarker of several diseases, including allergic rhinitis; autoimmune disease (multiple); cardiovascular system disease (multiple); diabetes mellitus (multiple); and hematologic cancer (multiple).

Mouse Cd40lg Target Information

Mouse Cd40lg Predicted Functions

Predicted to enable CD40 receptor binding activity and integrin binding activity. Involved in isotype switching. Acts upstream of or within B cell differentiation and regulation of immunoglobulin production. Located in external side of plasma membrane. Is expressed in Meckel’s cartilage; dorsal root ganglion; and limb. Used to study inflammatory bowel disease and systemic lupus erythematosus. Human ortholog(s) of this gene implicated in several diseases, including Kawasaki disease; artery disease (multiple); autoimmune disease (multiple); combined T cell and B cell immunodeficiency (multiple); and hematologic cancer (multiple). Orthologous to human CD40LG (CD40 ligand).

Rat Cd40lg Target Information

Rat Cd40lg Predicted Functions

Enables CD40 receptor binding activity. Involved in several processes, including positive regulation of B cell proliferation; positive regulation of MHC class II biosynthetic process; and positive regulation of cytokine production. Located in several cellular components, including cell body; cell surface; and extracellular space. Used to study Ehrlich tumor carcinoma; artery disease (multiple); autoimmune disease (multiple); thrombosis; and vitamin B12 deficiency. Biomarker of atherosclerosis; coronary artery disease; perinatal necrotizing enterocolitis; periodontal disease; and pulmonary hypertension. Human ortholog(s) of this gene implicated in several diseases, including Kawasaki disease; autoimmune disease (multiple); combined T cell and B cell immunodeficiency (multiple); hematologic cancer (multiple); and lupus nephritis. Orthologous to human CD40LG (CD40 ligand).

CD40LG Target News

    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)

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