Rat IgA ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	CD79A
SPECIES	Rat
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	0.48 ng/mL
Range	15.63 ng/mL – 1000 ng/mL
Assay Time	3.5 h
Recovery	82% – 105%
Average Recovery	0.95

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	0.48 ng/mL
Range	15.63 ng/mL – 1000 ng/mL
Assay Time	3.5 h
Recovery	82% – 105%
Average Recovery	0.95
Intra Precision	2.7% – 7.9%
Inter-Precision	3.9% – 8.6%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IgA monoclonal antibody Rat IgA freeze-dried standard IgA detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) technique. Specific anti-rat IgA antibodies are pre-coated on a high-affinity ELISA plate. Standard samples and test samples are added to the wells of the ELISA plate. After incubation, the IgA present in the sample binds to the solid-phase antibody. After washing to remove unbound substances, HRP-labeled detection antibodies are added and incubated. After washing to remove unbound detection antibodies, the TMB chromogenic substrate is added for color development while avoiding light. The intensity of the color reaction is directly proportional to the concentration of IgA in the sample. The reaction is terminated by adding a stop solution, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

CD79A

CD79A Target Infomation Overview

Target Symbol: CD79A, CD79a molecule
Gene Groups: CD molecules; V-set domain containing
Alias: MB-1
Previous Names: IGA
Alias Names: B-cell antigen receptor complex-associated protein alpha chain; CD79A antigen (immunoglobulin-associated alpha); CD79a molecule, immunoglobulin-associated alpha

CD79A, CD79a molecule Target Infomation by Species

HumanMouseRat

Human CD79A Target Information

Target Symbol: CD79A, CD79a molecule
Alias:
- B-cell antigen receptor complex-associated protein alpha chain
- CD79a antigen (immunoglobulin-associated alpha)
- CD79a molecule, immunoglobulin-associated alpha
- ig-alpha
- IGA
- MB-1
- MB-1 membrane glycoprotein
- membrane-bound immunoglobulin-associated protein
- surface IgM-associated protein
NCBI_Gene: 973
UniProtKB: P11912

Human CD79A Predicted Functions

Enables identical protein binding activity. Predicted to be involved in B cell differentiation; B cell proliferation; and B cell receptor signaling pathway. Predicted to be located in membrane raft and multivesicular body. Predicted to be part of B cell receptor complex. Predicted to be active in external side of plasma membrane. Implicated in agammaglobulinemia.

Mouse Cd79a Target Information

Target Symbol: Cd79a, CD79A antigen (immunoglobulin-associated alpha)
Alias:
- aimmunoglobulin-associated alpha
- CD79a antigen
- Ig alpha
- Ig-alpha
- Iga
- Igalpha
- immunoglobulin-associated alpha
- Ly-54
- Ly54
- lymphocyte antigen 54
- mb-1
NCBI_Gene: 12518
- UniprotKB: P11911

Mouse Cd79a Predicted Functions

Predicted to enable identical protein binding activity. Involved in B cell differentiation and B cell proliferation. Acts upstream of or within B cell receptor signaling pathway. Located in external side of plasma membrane; membrane raft; and multivesicular body. Part of B cell receptor complex. Is expressed in liver. Human ortholog(s) of this gene implicated in agammaglobulinemia. Orthologous to human CD79A (CD79a molecule).

Rat Cd79a Target Information

Target Symbol: Cd79a, CD79a molecule
Alias:
- -cell antigen receptor complex-associated protein alpha chain-like
- B-cell antigen receptor complex-associated protein alpha chain
- B-cell antigen receptor complex-associated protein alpha chain-like
- CD79A antigen (immunoglobulin-associated alpha)
- Cd79a molecule-like
- Cd79a molecule, immunoglobulin-associated alpha
- Cd79al
- LOC100913063
NCBI_Gene: 100913063
- UniprotKB: F1MAC3
- UniprotKB: M0R9P4

Rat Cd79a Predicted Functions

Predicted to enable identical protein binding activity. Predicted to be involved in B cell differentiation and B cell proliferation. Predicted to act upstream of or within B cell receptor signaling pathway. Predicted to be located in external side of plasma membrane; membrane raft; and multivesicular body. Predicted to be part of B cell receptor complex. Human ortholog(s) of this gene implicated in agammaglobulinemia. Orthologous to human CD79A (CD79a molecule).