Human ACE2 ELISA Kit

$350.00$450.00

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TARGETSACE2
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity29.60 pg/mL
Range0.31 ng/mL – 20 ng/mL
Assay Time3.5 h
Recovery98% – 121%
Average Recovery1.08
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity29.60 pg/mL
Range0.31 ng/mL – 20 ng/mL
Assay Time3.5 h
Recovery98% – 121%
Average Recovery1.08
Intra Precision3.1% – 4.9%
Inter-Precision3.6%-4.2%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-ACE2 monoclonal antibody
Human ACE2 freeze-dried standard
ACE2 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human ACE2 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the ACE2 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of ACE2 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

ACE2

ACE2 Target Infomation Overview

  • Target Symbol: ACE2, angiotensin converting enzyme 2
  • Alias: ACEH
  • Alias Names: peptidyl-dipeptidase A; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin I converting enzyme 2

ACE2, angiotensin converting enzyme 2 Target Infomation by Species

HumanMouseRat

Human ACE2 Target Information

  • Target Symbol: ACE2, angiotensin converting enzyme 2
  • Alias:
    • ACE-related carboxypeptidase
    • ACEH
    • angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
    • angiotensin I converting enzyme 2
    • angiotensin-converting enzyme 2
    • angiotensin-converting enzyme homolog
    • angiotensin-converting enzyme-related carboxypeptidase
    • DKFZp434A014
    • metalloprotease MPROT15
    • peptidyl-dipeptidase A
    • truncated angiotensin converting enzyme 2
  • NCBI_Gene: 59272
  • UniProtKB: Q9BYF1

Human ACE2 Predicted Functions

Enables identical protein binding activity; peptidase activity; and virus receptor activity. Involved in several processes, including angiotensin-mediated drinking behavior; positive regulation of gap junction assembly; and regulation of transmembrane transport. Located in cell surface; extracellular space; and plasma membrane. Part of apical plasma membrane and brush border membrane. Implicated in several diseases, including avian influenza; cerebral malaria; hypertension (multiple); respiratory syncytial virus infectious disease; and severe acute respiratory syndrome. Biomarker of congestive heart failure; hypertrophic cardiomyopathy; and pancreatic ductal carcinoma.

Mouse Ace2 Target Information

Mouse Ace2 Predicted Functions

Predicted to enable identical protein binding activity; peptidase activity; and virus receptor activity. Involved in positive regulation of cardiac muscle contraction and tryptophan transport. Located in brush border membrane and cytoplasm. Is expressed in several structures, including brain; cardiovascular system; extraembryonic component; genitourinary system; and respiratory system. Used to study COVID-19 and congestive heart failure. Human ortholog(s) of this gene implicated in several diseases, including avian influenza; cerebral malaria; hypertension (multiple); respiratory syncytial virus infectious disease; and severe acute respiratory syndrome. Orthologous to human ACE2 (angiotensin converting enzyme 2).

Rat Ace2 Target Information

Rat Ace2 Predicted Functions

Enables carboxypeptidase activity. Involved in maternal process involved in female pregnancy; negative regulation of ERK1 and ERK2 cascade; and negative regulation of smooth muscle cell proliferation. Located in extracellular space and membrane. Used to study glaucoma; myocardial infarction; and thrombosis. Biomarker of hypertension; lung disease; middle cerebral artery infarction; myocardial infarction; and primary biliary cholangitis. Human ortholog(s) of this gene implicated in several diseases, including avian influenza; cerebral malaria; hypertension (multiple); respiratory syncytial virus infectious disease; and severe acute respiratory syndrome. Orthologous to human ACE2 (angiotensin converting enzyme 2).

ACE2 Target News

    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Lung Disease (Asthma & COPD)
    Obesity Diabetes
    Sepsis

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