Human CCL5/RANTES ELISA Kit

$350.00$450.00

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TARGETSCCL5
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity0.53 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery84% – 117%
Average Recovery0.97
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity0.53 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery84% – 117%
Average Recovery0.97
Intra Precision6.9%-7.8%
Inter-Precision5.5% – 8.3%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CCL5/RANTESA monoclonal antibody
Human CCL5/RANTESA freeze-dried standard
CCL5/RANTESA detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human CCL5 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, CCL5 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CCL5 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

CCL5

CCL5 Target Infomation Overview

  • Target Symbol: CCL5, C-C motif chemokine ligand 5
  • Gene Groups: Chemokine ligands
  • Alias: RANTES; SISd; TCP228; MGC17164
  • Previous Names: D17S136E; SCYA5
  • Alias Names: T-cell specific protein p288; T-cell specific RANTES protein; SIS-delta; regulated upon activation, normally T-expressed, and presumably secreted; beta-chemokine RANTES; small inducible cytokine subfamily A (Cys-Cys), member 5; small inducible cytokine A5 (RANTES); chemokine (C-C motif) ligand 5

CCL5, C-C motif chemokine ligand 5 Target Infomation by Species

HumanMouseRat

Human CCL5 Target Information

  • Target Symbol: CCL5, C-C motif chemokine ligand 5
  • Alias:
    • beta-chemokine RANTES
    • C-C motif chemokine 5
    • chemokine (C-C motif) ligand 5
    • D17S136E
    • eoCP
    • eosinophil chemotactic cytokine
    • MGC17164
    • RANTES
    • regulated upon activation, normally T-expressed, and presumably secreted
    • SCYA5
    • SIS-delta
    • SISd
    • small inducible cytokine A5 (RANTES)
    • small inducible cytokine subfamily A (Cys-Cys), member 5
    • small-inducible cytokine A5
    • t cell-specific protein P228
    • T-cell specific protein p288
    • T-cell-specific protein RANTES
    • TCP228
  • NCBI_Gene: 6352
  • UniProtKB: P13501

Human CCL5 Predicted Functions

Enables several functions, including enzyme activator activity; phosphatidylinositol phospholipase C activity; and signaling receptor binding activity. Involved in several processes, including cellular response to cytokine stimulus; positive regulation of cell migration; and positive regulation of cell-cell adhesion. Acts upstream of or within positive regulation of T cell migration. Predicted to be located in cytoplasm. Predicted to be active in extracellular space. Implicated in several diseases, including hepatitis B; hepatitis C; liver disease (multiple); pulmonary tuberculosis; and severe acute respiratory syndrome. Biomarker of several diseases, including fatty liver disease (multiple); glucose metabolism disease (multiple); hypertension (multiple); liver cirrhosis (multiple); and lung disease (multiple).

Mouse Ccl5 Target Information

Mouse Ccl5 Predicted Functions

Enables CCR1 chemokine receptor binding activity. Involved in negative regulation of macrophage apoptotic process and positive regulation of monocyte chemotaxis. Acts upstream of or within inflammatory response; positive regulation of epithelial cell proliferation; and response to tumor necrosis factor. Located in cytoplasm and extracellular space. Is expressed in several structures, including central nervous system; gonad; hemolymphoid system; late tubule; and retina. Human ortholog(s) of this gene implicated in several diseases, including hepatitis B; hepatitis C; liver disease (multiple); pulmonary tuberculosis; and severe acute respiratory syndrome. Orthologous to human CCL5 (C-C motif chemokine ligand 5).

Rat Ccl5 Target Information

  • Target Symbol: Ccl5, C-C motif chemokine ligand 5
  • Alias:
    • C-C motif chemokine 5
    • chemokine (C-C motif) ligand 5
    • RANTES
    • regulated upon activation normal T-cell expressed and secreted
    • Scya5
    • SIS-delta
    • small inducible cytokine A5
    • small inducible cytokine A5 (RANTES)
    • small-inducible cytokine A5
    • T-cell-specific protein RANTES
  • NCBI_Gene: 81780

Rat Ccl5 Predicted Functions

Enables chemokine activity and heparin binding activity. Involved in several processes, including cellular response to organonitrogen compound; lymphocyte migration; and positive regulation of leukocyte chemotaxis. Located in extracellular space. Used to study Chagas disease; bronchiolitis obliterans; kidney disease; non-alcoholic fatty liver disease; and orchitis. Biomarker of several diseases, including alcoholic hepatitis; artery disease (multiple); cardiac arrest; lung disease (multiple); and uveitis (multiple). Human ortholog(s) of this gene implicated in several diseases, including hepatitis B; hepatitis C; liver disease (multiple); pulmonary tuberculosis; and severe acute respiratory syndrome. Orthologous to human CCL5 (C-C motif chemokine ligand 5).

CCL5 Target News

    12.469
    Chordoma recruits and polarizes tumor-associated macrophages via secreting CCL5 to promote malignant progression
    Journal for ImmunoTherapy of Cancer
    4.8
    MiR-9 promotes G-MDSC recruitment and tumor proliferation by targeting SOCS3 in breast cancer
    FASEB JOURNAL
    Allergic Reaction
    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Lung Disease (Asthma & COPD)
    Multiple Sclerosis
    Osteoarthrosis & Rheumatoid Arthritis
    Sepsis
    Systemic Lupus Erythematosus
    Transplantation

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