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Human CD19 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 50 μL 1×Assay Buffer and 50 μL sample |
| Sensitivity | 18.93 pg/mL |
| Range | 0.94 ng/mL – 60 ng/mL |
| Assay Time | 3.5 h |
| Recovery | 80% – 125% |
| Average Recovery | 0.98 |
| Intra Precision | 3.3% – 7.8% |
| Inter-Precision | 1.1%-5.4% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CD19 monoclonal antibody Human CD19 freeze-dried standard CD19 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human CD19 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, CD19 present in the samples binds to the solid-phase antibody and the detection antibody. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CD19 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
CD19
CD19 Target Infomation Overview
- Target Symbol: CD19, CD19 molecule
- Gene Groups: CD molecules; Immunoglobulin like domain containing; Minor histocompatibility antigens
- Alias Names: CD19 antigen
CD19, CD19 molecule Target Infomation by Species
HumanMouseRat
Human CD19 Target Information
- Target Symbol: CD19, CD19 molecule
- Alias:
- B-lymphocyte antigen CD19
- B-lymphocyte surface antigen B4
- B4
- cd19 antigen
- CVID3
- differentiation antigen CD19
- MGC12802
- T-cell surface antigen Leu-12
- NCBI_Gene: 930
- UniProtKB: P15391
Human CD19 Predicted Functions
Involved in several processes, including B cell proliferation involved in immune response; positive regulation of phosphatidylinositol 3-kinase activity; and positive regulation of release of sequestered calcium ion into cytosol. Located in external side of plasma membrane. Is integral component of plasma membrane. Part of protein-containing complex. Implicated in common variable immunodeficiency.
Mouse Cd19 Target Information
- Target Symbol: Cd19, CD19 antigen
- Alias:
- AW495831
- expressed sequence AW495831
- NCBI_Gene: 12478
Mouse Cd19 Predicted Functions
Involved in several processes, including B-1 B cell differentiation; positive regulation of phosphatidylinositol 3-kinase activity; and positive regulation of release of sequestered calcium ion into cytosol. Acts upstream of or within B cell receptor signaling pathway. Located in external side of plasma membrane. Is integral component of plasma membrane. Is expressed in liver and spleen. Human ortholog(s) of this gene implicated in common variable immunodeficiency. Orthologous to human CD19 (CD19 molecule).
Rat Cd19 Target Information
- Target Symbol: Cd19, CD19 molecule
- Alias:
- B-lymphocyte antigen CD19
- CD19 antigen
- LOC365367
- MGC109570
- NCBI_Gene: 365367
Rat Cd19 Predicted Functions
Predicted to be involved in several processes, including B cell activation; B cell receptor signaling pathway; and positive regulation of phosphatidylinositol 3-kinase activity. Predicted to act upstream of or within positive regulation of release of sequestered calcium ion into cytosol. Predicted to be integral component of plasma membrane. Predicted to be part of protein-containing complex. Predicted to be active in external side of plasma membrane. Human ortholog(s) of this gene implicated in common variable immunodeficiency. Orthologous to human CD19 (CD19 molecule).
