Human CD19 ELISA Kit

$245.00 – $350.00

Related Target	CD19
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL 1×Assay Buffer and 50 μL sample
Sensitivity	18.93 pg/mL
Array Range	0.94 ng/mL – 60 ng/mL
Assay Time	3.5 h
Recovery	80% – 125%
Average Recovery	98%

ELISA Kit Detail Information

Related Target	CD19
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL 1×Assay Buffer and 50 μL sample
Sensitivity	18.93 pg/mL
Array Range	0.94 ng/mL – 60 ng/mL
Assay Time	3.5 h
Recovery	80% – 125%
Average Recovery	98%
Intra Precision	3.3% – 7.8%
Inter Precision	1.1% - 5.4%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CD19 monoclonal antibody Human CD19 freeze-dried standard CD19 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human CD19 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, CD19 present in the samples binds to the solid-phase antibody and the detection antibody. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CD19 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

CD19

CD19 Target Infomation Overview

Target Symbol: CD19, CD19 molecule
Gene Groups: CD molecules; Immunoglobulin like domain containing; Minor histocompatibility antigens
Alias Names: CD19 antigen

CD19, CD19 molecule Target Infomation by Species

Human
Mouse
Rat

Human CD19 Target Information

Target Symbol: CD19, CD19 molecule
Alias:
- B-lymphocyte antigen CD19
- B-lymphocyte surface antigen B4
- B4
- cd19 antigen
- CVID3
- differentiation antigen CD19
- MGC12802
- T-cell surface antigen Leu-12
NCBI_Gene: 930
UniProtKB: P15391

Human CD19 Predicted Functions

Involved in several processes, including B cell proliferation involved in immune response; positive regulation of phosphatidylinositol 3-kinase activity; and positive regulation of release of sequestered calcium ion into cytosol. Located in external side of plasma membrane. Is integral component of plasma membrane. Part of protein-containing complex. Implicated in common variable immunodeficiency.

Mouse Cd19 Target Information

Target Symbol: Cd19, CD19 antigen
Alias:
- AW495831
- expressed sequence AW495831
NCBI_Gene: 12478

Mouse Cd19 Predicted Functions

Involved in several processes, including B-1 B cell differentiation; positive regulation of phosphatidylinositol 3-kinase activity; and positive regulation of release of sequestered calcium ion into cytosol. Acts upstream of or within B cell receptor signaling pathway. Located in external side of plasma membrane. Is integral component of plasma membrane. Is expressed in liver and spleen. Human ortholog(s) of this gene implicated in common variable immunodeficiency. Orthologous to human CD19 (CD19 molecule).

Rat Cd19 Target Information

Target Symbol: Cd19, CD19 molecule
Alias:
- B-lymphocyte antigen CD19
- CD19 antigen
- LOC365367
- MGC109570
NCBI_Gene: 365367
- UniprotKB: F1LNH2
- UniprotKB: Q5FVF7

Rat Cd19 Predicted Functions

Predicted to be involved in several processes, including B cell activation; B cell receptor signaling pathway; and positive regulation of phosphatidylinositol 3-kinase activity. Predicted to act upstream of or within positive regulation of release of sequestered calcium ion into cytosol. Predicted to be integral component of plasma membrane. Predicted to be part of protein-containing complex. Predicted to be active in external side of plasma membrane. Human ortholog(s) of this gene implicated in common variable immunodeficiency. Orthologous to human CD19 (CD19 molecule).

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