Human Cystatin C ELISA Kit

$350.00$450.00

Download:
TARGETSCST3
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity10.57 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery85% – 115%
Average Recovery0.98
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity10.57 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery85% – 115%
Average Recovery0.98
Intra Precision3.3% – 7.9%
Inter-Precision3.1% – 6.2%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Cystatin C monoclonal antibody
Human Cystatin C freeze-dried standard
Cystatin C detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Cystatin C antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Cystatin C present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Cystatin C in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

CST3

CST3 Target Infomation Overview

  • Target Symbol: CST3, cystatin C
  • Gene Groups: Cystatins, type 2
  • Alias Names: cystatin C (amyloid angiopathy and cerebral hemorrhage)

CST3, cystatin C Target Infomation by Species

HumanMouseRat

Human CST3 Target Information

  • Target Symbol: CST3, cystatin C
  • Alias:
    • ARMD11
    • bA218C14.4 (cystatin C)
    • cystatin 3
    • cystatin C (amyloid angiopathy and cerebral hemorrhage)
    • cystatin-3
    • cystatin-C
    • epididymis secretory protein Li 2
    • gamma-trace
    • HEL-S-2
    • MGC117328
    • neuroendocrine basic polypeptide
    • post-gamma-globulin
  • NCBI_Gene: 1471
  • UniProtKB: P01034

Human CST3 Predicted Functions

Enables several functions, including amyloid-beta binding activity; cysteine-type endopeptidase inhibitor activity; and identical protein binding activity. Involved in several processes, including negative regulation of blood vessel remodeling; negative regulation of catabolic process; and negative regulation of extracellular matrix disassembly. Located in Golgi apparatus; extracellular space; and plasma membrane. Implicated in CST3-related cerebral amyloid angiopathy and age related macular degeneration 11. Biomarker of several diseases, including artery disease (multiple); autoimmune disease of the nervous system (multiple); brain ischemia; obesity; and type 2 diabetes mellitus.

Mouse Cst3 Target Information

Mouse Cst3 Predicted Functions

Predicted to enable several functions, including amyloid-beta binding activity; cysteine-type endopeptidase inhibitor activity; and identical protein binding activity. Predicted to be involved in several processes, including negative regulation of cell death; positive regulation of DNA replication; and response to oxidative stress. Predicted to act upstream of or within negative regulation of peptidase activity. Located in collagen-containing extracellular matrix and extracellular space. Is expressed in several structures, including central nervous system; early conceptus; heart; reproductive system; and visual system. Human ortholog(s) of this gene implicated in CST3-related cerebral amyloid angiopathy and age related macular degeneration 11. Orthologous to several human genes including CST3 (cystatin C).

Rat Cst3 Target Information

Rat Cst3 Predicted Functions

Enables peptidase inhibitor activity and protease binding activity. Involved in several processes, including animal organ development; circadian sleep/wake cycle, REM sleep; and embryo implantation. Located in several cellular components, including multivesicular body; neuronal cell body; and nuclear membrane. Biomarker of intracranial aneurysm; iron deficiency anemia; and obesity. Human ortholog(s) of this gene implicated in CST3-related cerebral amyloid angiopathy and age related macular degeneration 11. Orthologous to several human genes including CST3 (cystatin C).

CST3 Target News

    5.6
    Microcystin-LR-Induced Interaction between M2 Tumor-Associated Macrophage and Colorectal Cancer Cell Promotes Colorectal Cancer Cell Migration through Regulating the Expression of TGF-β1 and CST3
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
    2.3
    Clinical value of combined plasma brain natriuretic peptide and serum cystatin C measurement on the prediction of heart failure in patients after acute myocardial infarction
    BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
    Obesity Diabetes
    Osteoporosis
    Sepsis
    Transplantation

    Related Products

    more +

    EK1392

    $350.00$450.00

    EK1C01

    $350.00$450.00

    EK1242

    $350.00$450.00

    Same Target