Human Cystatin C ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	CST3
SPECIES	Human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	10.57 pg/mL
Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	85% – 115%
Average Recovery	0.98

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	10.57 pg/mL
Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	85% – 115%
Average Recovery	0.98
Intra Precision	3.3% – 7.9%
Inter-Precision	3.1% – 6.2%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Cystatin C monoclonal antibody Human Cystatin C freeze-dried standard Cystatin C detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Cystatin C antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Cystatin C present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Cystatin C in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

CST3

CST3 Target Infomation Overview

Target Symbol: CST3, cystatin C
Gene Groups: Cystatins, type 2
Alias Names: cystatin C (amyloid angiopathy and cerebral hemorrhage)

CST3, cystatin C Target Infomation by Species

HumanMouseRat

Human CST3 Target Information

Target Symbol: CST3, cystatin C
Alias:
- ARMD11
- bA218C14.4 (cystatin C)
- cystatin 3
- cystatin C (amyloid angiopathy and cerebral hemorrhage)
- cystatin-3
- cystatin-C
- epididymis secretory protein Li 2
- gamma-trace
- HEL-S-2
- MGC117328
- neuroendocrine basic polypeptide
- post-gamma-globulin
NCBI_Gene: 1471
UniProtKB: P01034

Human CST3 Predicted Functions

Enables several functions, including amyloid-beta binding activity; cysteine-type endopeptidase inhibitor activity; and identical protein binding activity. Involved in several processes, including negative regulation of blood vessel remodeling; negative regulation of catabolic process; and negative regulation of extracellular matrix disassembly. Located in Golgi apparatus; extracellular space; and plasma membrane. Implicated in CST3-related cerebral amyloid angiopathy and age related macular degeneration 11. Biomarker of several diseases, including artery disease (multiple); autoimmune disease of the nervous system (multiple); brain ischemia; obesity; and type 2 diabetes mellitus.

Mouse Cst3 Target Information

Mouse Cst3 Predicted Functions

Predicted to enable several functions, including amyloid-beta binding activity; cysteine-type endopeptidase inhibitor activity; and identical protein binding activity. Predicted to be involved in several processes, including negative regulation of cell death; positive regulation of DNA replication; and response to oxidative stress. Predicted to act upstream of or within negative regulation of peptidase activity. Located in collagen-containing extracellular matrix and extracellular space. Is expressed in several structures, including central nervous system; early conceptus; heart; reproductive system; and visual system. Human ortholog(s) of this gene implicated in CST3-related cerebral amyloid angiopathy and age related macular degeneration 11. Orthologous to several human genes including CST3 (cystatin C).

Rat Cst3 Target Information

Target Symbol: Cst3, cystatin C
Alias:
- CYSC
- cystatin 3
- Cystatin C (cysteine proteinase inhibitor)
- cystatin-3
- cystatin-C
- MGC105556
NCBI_Gene: 25307
UniProtKB: P14841

Rat Cst3 Predicted Functions

Enables peptidase inhibitor activity and protease binding activity. Involved in several processes, including animal organ development; circadian sleep/wake cycle, REM sleep; and embryo implantation. Located in several cellular components, including multivesicular body; neuronal cell body; and nuclear membrane. Biomarker of intracranial aneurysm; iron deficiency anemia; and obesity. Human ortholog(s) of this gene implicated in CST3-related cerebral amyloid angiopathy and age related macular degeneration 11. Orthologous to several human genes including CST3 (cystatin C).