Human IL-23 ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	IL23A
SPECIES	Human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL 1×Assay Buffer and 50 μL sample
Sensitivity	1.82 pg/mL
Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	86% – 110%
Average Recovery	0.99

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL 1×Assay Buffer and 50 μL sample
Sensitivity	1.82 pg/mL
Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	86% – 110%
Average Recovery	0.99
Intra Precision	3.4% – 5.3%
Inter-Precision	3.4% – 4.8%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-23 monoclonal antibody Human IL-23 freeze-dried standard IL-23 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-23 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-23 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-23 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

IL23A

IL23A Target Infomation Overview

Target Symbol: IL23A, interleukin 23 subunit alpha
Gene Groups: Interleukins
Alias: SGRF; IL23P19; IL-23; IL-23A; P19
Alias Names: interleukin-six, G-CSF related factor; interleukin 23, alpha subunit p19

IL23A, interleukin 23 subunit alpha Target Infomation by Species

HumanMouseRat

Human IL23A Target Information

Target Symbol: IL23A, interleukin 23 subunit alpha
Alias:
- IL-23
- IL-23 subunit alpha
- IL-23-A
- IL-23A
- IL-23p19
- IL23P19
- interleukin 23 p19 subunit
- interleukin 23, alpha subunit p19
- interleukin-23 subunit alpha
- interleukin-23 subunit p19
- interleukin-six, G-CSF related factor
- JKA3 induced upon T-cell activation
- MGC79388
- P19
- SGRF
NCBI_Gene: 51561
UniProtKB: Q9NPF7

Human IL23A Predicted Functions

Contributes to interleukin-23 receptor binding activity. Involved in several processes, including positive regulation of cytokine production; positive regulation of lymphocyte proliferation; and positive regulation of peptidyl-tyrosine phosphorylation. Part of interleukin-23 complex.

Mouse Il23a Target Information

Target Symbol: Il23a, interleukin 23, alpha subunit p19
Alias:
- IL-23
- IL-23p19
- p19
NCBI_Gene: 83430
- UniprotKB: Q9EQ14

Mouse Il23a Predicted Functions

Contributes to interleukin-23 receptor binding activity. Involved in positive regulation of defense response to virus by host; positive regulation of immune response; and positive regulation of neutrophil chemotaxis. Acts upstream of or within T cell proliferation and positive regulation of transcription by RNA polymerase II. Located in extracellular space. Part of interleukin-23 complex. Is expressed in decidua; decidua basalis; embryo; and extraembryonic component. Orthologous to human IL23A (interleukin 23 subunit alpha).

Rat Il23a Target Information

Target Symbol: Il23a, interleukin 23 subunit alpha
Alias:
- IL-23 subunit alpha
- IL-23-A
- IL-23p19
- Interleukin 23, alpha subunit p19
- interleukin-23 subunit alpha
- interleukin-23 subunit p19
- MGC114275
NCBI_Gene: 155140
UniProtKB: Q91Z84

Rat Il23a Predicted Functions

Predicted to enable cytokine activity. Predicted to contribute to interleukin-23 receptor binding activity. Predicted to be involved in several processes, including positive regulation of cytokine production; positive regulation of lymphocyte activation; and positive regulation of peptidyl-tyrosine phosphorylation. Predicted to act upstream of or within T cell proliferation and positive regulation of transcription by RNA polymerase II. Predicted to be located in extracellular space. Predicted to be part of interleukin-23 complex. Orthologous to human IL23A (interleukin 23 subunit alpha).