EK223

Mouse IL-23 ELISA Kit

$245.00$350.00

Related Target
Speciesmouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity0.31 pg/mL
Array Range1.56 pg/mL – 100 pg/mL
Assay Time3.5 h
Recovery80% – 99%
Average Recovery91%

ELISA Kit Detail Information

Related Target
Speciesmouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity0.31 pg/mL
Array Range1.56 pg/mL – 100 pg/mL
Assay Time3.5 h
Recovery80% – 99%
Average Recovery91%
Intra Precision3.0% – 5.4%
Inter Precision4.4% – 6.4%
PlateDetachable 96-well plate
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-23 monoclonal antibody
Mouse IL-23 freeze-dried standard
IL-23 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IL-23 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-23 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-23 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

IL23A

IL23A Target Infomation Overview

  • Target Symbol: IL23A, interleukin 23 subunit alpha
  • Gene Groups: Interleukins
  • Alias: SGRF; IL23P19; IL-23; IL-23A; P19
  • Alias Names: interleukin-six, G-CSF related factor; interleukin 23, alpha subunit p19

IL23A, interleukin 23 subunit alpha Target Infomation by Species

  • Human
  • Mouse
  • Rat

Human IL23A Target Information

  • Target Symbol: IL23A, interleukin 23 subunit alpha
  • Alias:
    • IL-23
    • IL-23 subunit alpha
    • IL-23-A
    • IL-23A
    • IL-23p19
    • IL23P19
    • interleukin 23 p19 subunit
    • interleukin 23, alpha subunit p19
    • interleukin-23 subunit alpha
    • interleukin-23 subunit p19
    • interleukin-six, G-CSF related factor
    • JKA3 induced upon T-cell activation
    • MGC79388
    • P19
    • SGRF
  • NCBI_Gene: 51561
  • UniProtKB: Q9NPF7

Human IL23A Predicted Functions

Contributes to interleukin-23 receptor binding activity. Involved in several processes, including positive regulation of cytokine production; positive regulation of lymphocyte proliferation; and positive regulation of peptidyl-tyrosine phosphorylation. Part of interleukin-23 complex.

Mouse Il23a Target Information

Mouse Il23a Predicted Functions

Contributes to interleukin-23 receptor binding activity. Involved in positive regulation of defense response to virus by host; positive regulation of immune response; and positive regulation of neutrophil chemotaxis. Acts upstream of or within T cell proliferation and positive regulation of transcription by RNA polymerase II. Located in extracellular space. Part of interleukin-23 complex. Is expressed in decidua; decidua basalis; embryo; and extraembryonic component. Orthologous to human IL23A (interleukin 23 subunit alpha).

Rat Il23a Target Information

  • Target Symbol: Il23a, interleukin 23 subunit alpha
  • Alias:
    • IL-23 subunit alpha
    • IL-23-A
    • IL-23p19
    • Interleukin 23, alpha subunit p19
    • interleukin-23 subunit alpha
    • interleukin-23 subunit p19
    • MGC114275
  • NCBI_Gene: 155140
  • UniProtKB: Q91Z84

Rat Il23a Predicted Functions

Predicted to enable cytokine activity. Predicted to contribute to interleukin-23 receptor binding activity. Predicted to be involved in several processes, including positive regulation of cytokine production; positive regulation of lymphocyte activation; and positive regulation of peptidyl-tyrosine phosphorylation. Predicted to act upstream of or within T cell proliferation and positive regulation of transcription by RNA polymerase II. Predicted to be located in extracellular space. Predicted to be part of interleukin-23 complex. Orthologous to human IL23A (interleukin 23 subunit alpha).

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