Mouse Granzyme B ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	GZMB
SPECIES	Mouse
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	10 μL
Sensitivity	0.91 pg/mL
Range	0.31 ng/mL – 20 ng/mL
Assay Time	3.5 h
Recovery	91% – 105%
Average Recovery	0.99

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	10 μL
Sensitivity	0.91 pg/mL
Range	0.31 ng/mL – 20 ng/mL
Assay Time	3.5 h
Recovery	91% – 105%
Average Recovery	0.99
Intra Precision	2.9%-3.9%
Inter-Precision	1.8% – 2.8%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Granzyme B monoclonal antibody Mouse Granzyme B freeze-dried standard Granzyme B detect Antibody HRP-labeled streptavidin Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse Granzyme B antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Granzyme B present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Granzyme B in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

GZMB

GZMB Target Infomation Overview

Target Symbol: GZMB, granzyme B
Gene Groups: Granule associated serine proteases of immune defence
Alias: CCPI; CGL-1; CSP-B; CGL1; CTSGL1; HLP; SECT
Previous Names: CTLA1; CSPB
Alias Names: fragmentin 2; cytotoxic serine protease B; cathepsin G-like 1; T-cell serine protease 1-3E; granzyme 2; cytotoxic T-lymphocyte-associated serine esterase 1; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)

GZMB, granzyme B Target Infomation by Species

HumanMouseRat

Human GZMB Target Information

Target Symbol: GZMB, granzyme B
Alias:
- C11
- cathepsin G-like 1
- CCPI
- CGL-1
- CGL1
- CSP-B
- CSPB
- CTLA-1
- CTLA1
- CTSGL1
- cytotoxic serine protease B
- cytotoxic T-lymphocyte proteinase 2
- cytotoxic T-lymphocyte-associated serine esterase 1
- fragmentin 2
- fragmentin-2
- granzyme 2
- granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
- granzyme-2
- HLP
- human lymphocyte protein
- lymphocyte protease
- SECT
- T-cell serine protease 1-3E
NCBI_Gene: 3002
UniProtKB: P10144

Human GZMB Predicted Functions

Enables serine-type endopeptidase activity. Involved in several processes, including granzyme-mediated programmed cell death signaling pathway; natural killer cell mediated cytotoxicity; and pyroptosis. Located in cytolytic granule. Biomarker of COVID-19; bronchiolitis obliterans; and chronic obstructive pulmonary disease.

Mouse Gzme Target Information

Target Symbol: Gzme, granzyme E
Alias:
- CCP3
- Ctla-6
- Ctla6
- cytotoxic T lymphocyte-associated protein 6
- MCSP-2
NCBI_Gene: 14942
- UniprotKB: P08884

Mouse Gzme Predicted Functions

Predicted to enable serine-type endopeptidase activity. Predicted to be involved in several processes, including granzyme-mediated programmed cell death signaling pathway; natural killer cell mediated cytotoxicity; and pyroptosis. Predicted to act upstream of or within cytolysis. Predicted to be located in cytolytic granule. Predicted to be active in cytoplasm and intracellular membrane-bounded organelle. Is expressed in extraembryonic component; placenta; spongiotrophoblast; and trophoblast giant cell. Orthologous to human GZMB (granzyme B).

Rat Gzmb Target Information

Target Symbol: Gzmb, granzyme B
Alias:
- fragmentin
- GLP I
- GLP III
- GLP-1
- granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
- granzyme-like protein 1
- granzyme-like protein I
- LOW QUALITY PROTEIN: granzyme-like protein 1
- MGC156565
- natural killer cell protease 1
- RNKP-1
NCBI_Gene: 171528

Rat Gzmb Predicted Functions

Enables serine-type endopeptidase activity. Involved in positive regulation of necroptotic process. Located in early endosome. Orthologous to human GZMB (granzyme B).