Mouse Granzyme B ELISA Kit

$350.00$450.00

TARGETS GZMB
SPECIES Mouse
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume 10 μL
Sensitivity 0.91 pg/mL
Range 0.31 ng/mL – 20 ng/mL
Assay Time 3.5 h
Recovery 91% – 105%
Average Recovery 0.99
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume 10 μL
Sensitivity 0.91 pg/mL
Range 0.31 ng/mL – 20 ng/mL
Assay Time 3.5 h
Recovery 91% – 105%
Average Recovery 0.99
Intra Precision 2.9%-3.9%
Inter-Precision 1.8% – 2.8%
Platform ELISA
Plate Detachable 96-well plate
Size 96T/48T
Storage If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery 4℃ blue ice transportation
Components 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Granzyme B monoclonal antibody
Mouse Granzyme B freeze-dried standard
Granzyme B detect Antibody
HRP-labeled streptavidin
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay Principle This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse Granzyme B antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Granzyme B present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Granzyme B in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

GZMB

GZMB Target Infomation Overview

  • Target Symbol: GZMB, granzyme B
  • Gene Groups: Granule associated serine proteases of immune defence
  • Alias: CCPI; CGL-1; CSP-B; CGL1; CTSGL1; HLP; SECT
  • Previous Names: CTLA1; CSPB
  • Alias Names: fragmentin 2; cytotoxic serine protease B; cathepsin G-like 1; T-cell serine protease 1-3E; granzyme 2; cytotoxic T-lymphocyte-associated serine esterase 1; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)

GZMB, granzyme B Target Infomation by Species

[su_tabs][su_tab title=”Human” disabled=”no” anchor=”” url=”” target=”blank” class=”tab-human”]

Human GZMB Target Information

  • Target Symbol: GZMB, granzyme B
  • Alias:
    • C11
    • cathepsin G-like 1
    • CCPI
    • CGL-1
    • CGL1
    • CSP-B
    • CSPB
    • CTLA-1
    • CTLA1
    • CTSGL1
    • cytotoxic serine protease B
    • cytotoxic T-lymphocyte proteinase 2
    • cytotoxic T-lymphocyte-associated serine esterase 1
    • fragmentin 2
    • fragmentin-2
    • granzyme 2
    • granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
    • granzyme-2
    • HLP
    • human lymphocyte protein
    • lymphocyte protease
    • SECT
    • T-cell serine protease 1-3E
  • NCBI_Gene: 3002
  • UniProtKB: P10144

Human GZMB Predicted Functions

Enables serine-type endopeptidase activity. Involved in several processes, including granzyme-mediated programmed cell death signaling pathway; natural killer cell mediated cytotoxicity; and pyroptosis. Located in cytolytic granule. Biomarker of COVID-19; bronchiolitis obliterans; and chronic obstructive pulmonary disease.

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[su_tab title=”Mouse” disabled=”no” anchor=”” url=”” target=”blank” class=”tab-mouse”]

Mouse Gzme Target Information

Mouse Gzme Predicted Functions

Predicted to enable serine-type endopeptidase activity. Predicted to be involved in several processes, including granzyme-mediated programmed cell death signaling pathway; natural killer cell mediated cytotoxicity; and pyroptosis. Predicted to act upstream of or within cytolysis. Predicted to be located in cytolytic granule. Predicted to be active in cytoplasm and intracellular membrane-bounded organelle. Is expressed in extraembryonic component; placenta; spongiotrophoblast; and trophoblast giant cell. Orthologous to human GZMB (granzyme B).

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[su_tab title=”Rat” disabled=”no” anchor=”” url=”” target=”blank” class=”tab-rat”]

Rat Gzmb Target Information

  • Target Symbol: Gzmb, granzyme B
  • Alias:
    • fragmentin
    • GLP I
    • GLP III
    • GLP-1
    • granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
    • granzyme-like protein 1
    • granzyme-like protein I
    • LOW QUALITY PROTEIN: granzyme-like protein 1
    • MGC156565
    • natural killer cell protease 1
    • RNKP-1
  • NCBI_Gene: 171528

Rat Gzmb Predicted Functions

Enables serine-type endopeptidase activity. Involved in positive regulation of necroptotic process. Located in early endosome. Orthologous to human GZMB (granzyme B).

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GZMB Target News

[catlist tags=”gzmb” template=targetnews thumbnail=yes thumbnail_class=”related-post-media clr” thumbnail_size=833]

8.2
IL-1β mediates Candida tropicalis-induced immunosuppressive function of MDSCs to foster colorectal cancer
Cell Communication and Signaling
6.9
Controlling the speed of antigens transport in dendritic cells improves humoral and cellular immunity for vaccine
BIOMEDICINE & PHARMACOTHERAPY
28.2
An inflammatory checkpoint generated by IL1RN splicing offers therapeutic opportunity for KRAS mutant intrahepatic cholangiocarcinoma
Cancer Discovery
6.4
Deubiquitinase inhibitor PR-619 potentiates colon cancer immunotherapy by inducing ferroptosis
IMMUNOLOGY
13.2
An mRNA-based broad-spectrum vaccine candidate confers cross-protection against heterosubtypic influenza A viruses
Emerging Microbes & Infections
5.6
Astragalus polysaccharides promote neural stem cells-derived oligodendrogenesis through attenuating CD8+T cell infiltration in experimental autoimmune encephalomyelitis
INTERNATIONAL IMMUNOPHARMACOLOGY
4.3
Damage-induced NAD release activates intestinal CD4+ and CD8+ T cell via P2X7R signaling
CELLULAR IMMUNOLOGY
Lung Disease (Asthma & COPD)
Multiple Sclerosis
Systemic Lupus Erythematosus
Transplantation
Tumor Marker

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