Mouse Granzyme B ELISA Kit

$350.00$450.00

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TARGETSGZMB
SPECIESMouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity0.91 pg/mL
Range0.31 ng/mL – 20 ng/mL
Assay Time3.5 h
Recovery91% – 105%
Average Recovery0.99
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity0.91 pg/mL
Range0.31 ng/mL – 20 ng/mL
Assay Time3.5 h
Recovery91% – 105%
Average Recovery0.99
Intra Precision2.9%-3.9%
Inter-Precision1.8% – 2.8%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Granzyme B monoclonal antibody
Mouse Granzyme B freeze-dried standard
Granzyme B detect Antibody
HRP-labeled streptavidin
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse Granzyme B antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Granzyme B present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Granzyme B in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

GZMB

GZMB Target Infomation Overview

  • Target Symbol: GZMB, granzyme B
  • Gene Groups: Granule associated serine proteases of immune defence
  • Alias: CCPI; CGL-1; CSP-B; CGL1; CTSGL1; HLP; SECT
  • Previous Names: CTLA1; CSPB
  • Alias Names: fragmentin 2; cytotoxic serine protease B; cathepsin G-like 1; T-cell serine protease 1-3E; granzyme 2; cytotoxic T-lymphocyte-associated serine esterase 1; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)

GZMB, granzyme B Target Infomation by Species

HumanMouseRat

Human GZMB Target Information

  • Target Symbol: GZMB, granzyme B
  • Alias:
    • C11
    • cathepsin G-like 1
    • CCPI
    • CGL-1
    • CGL1
    • CSP-B
    • CSPB
    • CTLA-1
    • CTLA1
    • CTSGL1
    • cytotoxic serine protease B
    • cytotoxic T-lymphocyte proteinase 2
    • cytotoxic T-lymphocyte-associated serine esterase 1
    • fragmentin 2
    • fragmentin-2
    • granzyme 2
    • granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
    • granzyme-2
    • HLP
    • human lymphocyte protein
    • lymphocyte protease
    • SECT
    • T-cell serine protease 1-3E
  • NCBI_Gene: 3002
  • UniProtKB: P10144

Human GZMB Predicted Functions

Enables serine-type endopeptidase activity. Involved in several processes, including granzyme-mediated programmed cell death signaling pathway; natural killer cell mediated cytotoxicity; and pyroptosis. Located in cytolytic granule. Biomarker of COVID-19; bronchiolitis obliterans; and chronic obstructive pulmonary disease.

Mouse Gzme Target Information

Mouse Gzme Predicted Functions

Predicted to enable serine-type endopeptidase activity. Predicted to be involved in several processes, including granzyme-mediated programmed cell death signaling pathway; natural killer cell mediated cytotoxicity; and pyroptosis. Predicted to act upstream of or within cytolysis. Predicted to be located in cytolytic granule. Predicted to be active in cytoplasm and intracellular membrane-bounded organelle. Is expressed in extraembryonic component; placenta; spongiotrophoblast; and trophoblast giant cell. Orthologous to human GZMB (granzyme B).

Rat Gzmb Target Information

  • Target Symbol: Gzmb, granzyme B
  • Alias:
    • fragmentin
    • GLP I
    • GLP III
    • GLP-1
    • granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
    • granzyme-like protein 1
    • granzyme-like protein I
    • LOW QUALITY PROTEIN: granzyme-like protein 1
    • MGC156565
    • natural killer cell protease 1
    • RNKP-1
  • NCBI_Gene: 171528

Rat Gzmb Predicted Functions

Enables serine-type endopeptidase activity. Involved in positive regulation of necroptotic process. Located in early endosome. Orthologous to human GZMB (granzyme B).

GZMB Target News

    Lung Disease (Asthma & COPD)
    Multiple Sclerosis
    Systemic Lupus Erythematosus
    Transplantation
    Tumor Marker

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