Human Granzyme B ELISA Kit
$245.00 – $350.00
| Related Target | |
|---|---|
| Species | human |
| Sample Type | Serum, plasma, cell culture supernatant, DMEM cell culture supernatant and other biological samples |
| Sample Volume | Serum, plasma: 20 μL, cell culture supernatant: 100 μL |
| Sensitivity | 3.54 pg/mL |
| Array Range | 15.63 pg/mL - 1000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 83% - 104% |
| Average Recovery | 94% |
ELISA Kit Detail Information
| Related Target | |
|---|---|
| Species | human |
| Sample Type | Serum, plasma, cell culture supernatant, DMEM cell culture supernatant and other biological samples |
| Sample Volume | Serum, plasma: 20 μL, cell culture supernatant: 100 μL |
| Sensitivity | 3.54 pg/mL |
| Array Range | 15.63 pg/mL - 1000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 83% - 104% |
| Average Recovery | 94% |
| Intra Precision | 5.5% - 7.0% |
| Inter Precision | 3.8% - 5.4% |
| Plate | Detachable 96-well Plate |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ Blue Ice Transport |
| Components | 1× Washing Buffer 1× Assay Buffer Stop Solution Substrate TMB Detect Antibody Standard Diluent Streptavidin-HRP Standard Microplate Wells |
| Assay Principle | This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human Granzyme B has been pre-coated onto a microplate. Standard, samples and biotin-linked detect antibody specific for Granzyme B are pipetted into the wells and Granzyme B present is bound by the immobilized antibody and detect antibody following incubation. After washing away any unbound substances, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of Granzyme B bound in the initial step. The color development is stopped and the intensity of the color is measured |
Related Targets
GZMB
GZMB Target Infomation Overview
- Target Symbol: GZMB, granzyme B
- Gene Groups: Granule associated serine proteases of immune defence
- Alias: CCPI; CGL-1; CSP-B; CGL1; CTSGL1; HLP; SECT
- Previous Names: CTLA1; CSPB
- Alias Names: fragmentin 2; cytotoxic serine protease B; cathepsin G-like 1; T-cell serine protease 1-3E; granzyme 2; cytotoxic T-lymphocyte-associated serine esterase 1; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
GZMB, granzyme B Target Infomation by Species
- Human
- Mouse
- Rat
Human GZMB Target Information
- Target Symbol: GZMB, granzyme B
- Alias:
- C11
- cathepsin G-like 1
- CCPI
- CGL-1
- CGL1
- CSP-B
- CSPB
- CTLA-1
- CTLA1
- CTSGL1
- cytotoxic serine protease B
- cytotoxic T-lymphocyte proteinase 2
- cytotoxic T-lymphocyte-associated serine esterase 1
- fragmentin 2
- fragmentin-2
- granzyme 2
- granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
- granzyme-2
- HLP
- human lymphocyte protein
- lymphocyte protease
- SECT
- T-cell serine protease 1-3E
- NCBI_Gene: 3002
- UniProtKB: P10144
Human GZMB Predicted Functions
Enables serine-type endopeptidase activity. Involved in several processes, including granzyme-mediated programmed cell death signaling pathway; natural killer cell mediated cytotoxicity; and pyroptosis. Located in cytolytic granule. Biomarker of COVID-19; bronchiolitis obliterans; and chronic obstructive pulmonary disease.
Mouse Gzme Target Information
- Target Symbol: Gzme, granzyme E
- Alias:
- CCP3
- Ctla-6
- Ctla6
- cytotoxic T lymphocyte-associated protein 6
- MCSP-2
- NCBI_Gene: 14942
Mouse Gzme Predicted Functions
Predicted to enable serine-type endopeptidase activity. Predicted to be involved in several processes, including granzyme-mediated programmed cell death signaling pathway; natural killer cell mediated cytotoxicity; and pyroptosis. Predicted to act upstream of or within cytolysis. Predicted to be located in cytolytic granule. Predicted to be active in cytoplasm and intracellular membrane-bounded organelle. Is expressed in extraembryonic component; placenta; spongiotrophoblast; and trophoblast giant cell. Orthologous to human GZMB (granzyme B).
Rat Gzmb Target Information
- Target Symbol: Gzmb, granzyme B
- Alias:
- fragmentin
- GLP I
- GLP III
- GLP-1
- granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
- granzyme-like protein 1
- granzyme-like protein I
- LOW QUALITY PROTEIN: granzyme-like protein 1
- MGC156565
- natural killer cell protease 1
- RNKP-1
- NCBI_Gene: 171528
Rat Gzmb Predicted Functions
Enables serine-type endopeptidase activity. Involved in positive regulation of necroptotic process. Located in early endosome. Orthologous to human GZMB (granzyme B).
Products with the Same Target - GZMB
Citations for Human Granzyme B ELISA Kit
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ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol
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ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects
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