Mouse Granzyme B ELISA Kit
$245.00 – $350.00
| Related Target | |
|---|---|
| Species | mouse |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 10 μL |
| Sensitivity | 0.91 pg/mL |
| Array Range | 0.31 ng/mL – 20 ng/mL |
| Assay Time | 3.5 h |
| Recovery | 91% – 105% |
| Average Recovery | 99% |
ELISA Kit Detail Information
| Related Target | |
|---|---|
| Species | mouse |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 10 μL |
| Sensitivity | 0.91 pg/mL |
| Array Range | 0.31 ng/mL – 20 ng/mL |
| Assay Time | 3.5 h |
| Recovery | 91% – 105% |
| Average Recovery | 99% |
| Intra Precision | 2.9% - 3.9% |
| Inter Precision | 1.8% – 2.8% |
| Plate | Detachable 96-well plate |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Granzyme B monoclonal antibody Mouse Granzyme B freeze-dried standard Granzyme B detect Antibody HRP-labeled streptavidin Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse Granzyme B antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Granzyme B present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Granzyme B in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Related Targets
GZMB
GZMB Target Infomation Overview
- Target Symbol: GZMB, granzyme B
- Gene Groups: Granule associated serine proteases of immune defence
- Alias: CCPI; CGL-1; CSP-B; CGL1; CTSGL1; HLP; SECT
- Previous Names: CTLA1; CSPB
- Alias Names: fragmentin 2; cytotoxic serine protease B; cathepsin G-like 1; T-cell serine protease 1-3E; granzyme 2; cytotoxic T-lymphocyte-associated serine esterase 1; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
GZMB, granzyme B Target Infomation by Species
- Human
- Mouse
- Rat
Human GZMB Target Information
- Target Symbol: GZMB, granzyme B
- Alias:
- C11
- cathepsin G-like 1
- CCPI
- CGL-1
- CGL1
- CSP-B
- CSPB
- CTLA-1
- CTLA1
- CTSGL1
- cytotoxic serine protease B
- cytotoxic T-lymphocyte proteinase 2
- cytotoxic T-lymphocyte-associated serine esterase 1
- fragmentin 2
- fragmentin-2
- granzyme 2
- granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
- granzyme-2
- HLP
- human lymphocyte protein
- lymphocyte protease
- SECT
- T-cell serine protease 1-3E
- NCBI_Gene: 3002
- UniProtKB: P10144
Human GZMB Predicted Functions
Enables serine-type endopeptidase activity. Involved in several processes, including granzyme-mediated programmed cell death signaling pathway; natural killer cell mediated cytotoxicity; and pyroptosis. Located in cytolytic granule. Biomarker of COVID-19; bronchiolitis obliterans; and chronic obstructive pulmonary disease.
Mouse Gzme Target Information
- Target Symbol: Gzme, granzyme E
- Alias:
- CCP3
- Ctla-6
- Ctla6
- cytotoxic T lymphocyte-associated protein 6
- MCSP-2
- NCBI_Gene: 14942
Mouse Gzme Predicted Functions
Predicted to enable serine-type endopeptidase activity. Predicted to be involved in several processes, including granzyme-mediated programmed cell death signaling pathway; natural killer cell mediated cytotoxicity; and pyroptosis. Predicted to act upstream of or within cytolysis. Predicted to be located in cytolytic granule. Predicted to be active in cytoplasm and intracellular membrane-bounded organelle. Is expressed in extraembryonic component; placenta; spongiotrophoblast; and trophoblast giant cell. Orthologous to human GZMB (granzyme B).
Rat Gzmb Target Information
- Target Symbol: Gzmb, granzyme B
- Alias:
- fragmentin
- GLP I
- GLP III
- GLP-1
- granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
- granzyme-like protein 1
- granzyme-like protein I
- LOW QUALITY PROTEIN: granzyme-like protein 1
- MGC156565
- natural killer cell protease 1
- RNKP-1
- NCBI_Gene: 171528
Rat Gzmb Predicted Functions
Enables serine-type endopeptidase activity. Involved in positive regulation of necroptotic process. Located in early endosome. Orthologous to human GZMB (granzyme B).
Products with the Same Target - GZMB
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