Human Betacellulin ELISA Kit

$350.00$450.00

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TARGETSBTC
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity0.45 pg/mL
Range3.91 pg/mL – 250 pg/mL
Assay Time3.5 h
Recovery89% – 115%
Average Recovery1.03
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity0.45 pg/mL
Range3.91 pg/mL – 250 pg/mL
Assay Time3.5 h
Recovery89% – 115%
Average Recovery1.03
Intra Precision4.1% – 4.9%
Inter-Precision0.6%-3.4%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Betacellulin monoclonal antibody
Human Betacellulin freeze-dried standard
Human Betacellulin detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Betacellulin antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the Betacellulin present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Betacellulin in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

BTC

BTC Target Infomation Overview

  • Target Symbol: BTC, betacellulin

BTC, betacellulin Target Infomation by Species

HumanMouseRat

Human BTC Target Information

Human BTC Predicted Functions

Predicted to enable epidermal growth factor receptor binding activity and growth factor activity. Predicted to be involved in several processes, including epidermal growth factor receptor signaling pathway; positive regulation of epidermal growth factor-activated receptor activity; and positive regulation of mitotic nuclear division. Predicted to act upstream of or within negative regulation of apoptotic process. Predicted to be located in clathrin-coated endocytic vesicle membrane and extracellular region. Predicted to be active in extracellular space. Implicated in type 1 diabetes mellitus and type 2 diabetes mellitus. Biomarker of insulinoma.

Mouse Btc Target Information

Mouse Btc Predicted Functions

Predicted to enable epidermal growth factor receptor binding activity and growth factor activity. Acts upstream of or within negative regulation of apoptotic process and positive regulation of cell population proliferation. Predicted to be located in membrane. Predicted to be active in extracellular space. Is expressed in lung; reproductive system; and skin. Human ortholog(s) of this gene implicated in type 1 diabetes mellitus and type 2 diabetes mellitus. Orthologous to human BTC (betacellulin).

Rat Btc Target Information

Rat Btc Predicted Functions

Enables epidermal growth factor receptor binding activity and growth factor activity. Involved in several processes, including positive regulation of fibroblast proliferation; positive regulation of mitotic nuclear division; and positive regulation of urine volume. Predicted to be integral component of membrane. Predicted to be active in extracellular space. Biomarker of otitis media. Human ortholog(s) of this gene implicated in type 1 diabetes mellitus and type 2 diabetes mellitus. Orthologous to human BTC (betacellulin).

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