Human Betacellulin ELISA Kit

$245.00 – $350.00

Related Target	BTC
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL
Sensitivity	0.45 pg/mL
Array Range	3.91 pg/mL – 250 pg/mL
Assay Time	3.5 h
Recovery	89% – 115%
Average Recovery	103%

ELISA Kit Detail Information

Related Target	BTC
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL
Sensitivity	0.45 pg/mL
Array Range	3.91 pg/mL – 250 pg/mL
Assay Time	3.5 h
Recovery	89% – 115%
Average Recovery	103%
Intra Precision	4.1% – 4.9%
Inter Precision	0.6% - 3.4%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Betacellulin monoclonal antibody Human Betacellulin freeze-dried standard Human Betacellulin detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Betacellulin antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the Betacellulin present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Betacellulin in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

BTC

No description available.

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