stylish - MULTI SCIENCES

2 - ELISA Weak/No Signal? Causes & Solutions for Reliable Results

Post published:April 8, 2025
Post category:ELISA FAQS
Post comments:0 Comments

Are you getting no signal or weak signals in your ELISA assays? This common problem can derail your research. Discover the key causes and proven solutions to restore your assay sensitivity: Top 10 Reasons for ELISA Signal Failure & How to Fix Them:

Mixed reagent batches
Improper storage
Expired reagents
Missing detection antibodies
Incorrect incubation
Wrong addition order
Contaminated reagents
Pipetting errors
Microsphere issues
Inadequate washing

Critical Tip: For one-step ELISAs, control humidity exposure and loading intervals to prevent microsphere degradation.

3 - ELISA Standard Curve Problems? Fix Poor Linearity & Gradient Issues

Post published:April 8, 2025
Post category:ELISA FAQS
Post comments:0 Comments

Is your ELISA standard curve showing poor linearity or no gradient distribution? This critical assay problem can compromise your data reliability. Learn the root causes and expert solutions: Top Causes & Solutions for Standard Curve Failures:

Incorrect incubation
Improper washing
Pipetting errors
Microsphere problems
Humidity exposure

Pro Tip: Always include a positive control to validate curve performance before sample testing. Why This Matters: A properly gradiented standard curve is essential for accurate quantification in Biomedical research, Clinical diagnostics and Drug development

A Brife Review of the Apoptosis Pathway

Post published:March 20, 2025
Post category:Apoptosis Technical Article
Post comments:0 Comments

Apoptosis occurs through two main pathways: extrinsic and intrinsic. The extrinsic pathway activates caspases via extracellular signals, while the intrinsic pathway involves cytochrome c release from mitochondria, forming an apoptosome with Apaf-1 and caspase-9 to activate caspase-3. Caspases, cysteine proteases, degrade key intracellular proteins, leading to apoptosis. They are divided into the ICE subfamily (involved in inflammation) and the CED-3 family (involved in apoptosis). The mitochondrial pathway is regulated by Bcl-2 family proteins, which control the permeability transition pore (PT pore) and cytochrome c release. Additional proteins like Smac, AIF, and Endo G also contribute to apoptosis. In Fas signaling, caspase-8 activation amplifies the apoptotic signal through Bid cleavage, particularly in type II cells.

Tumor necrosis factor (TNF): TNF-α & TNF-β

Post published:February 22, 2025
Post category:Target Tips
Post comments:0 Comments

The Tumor Necrosis Factor (TNF) family comprises cytokines that induce cell death (apoptosis). Key members include TNF-α and TNF-β. TNF-α, a pro-inflammatory cytokine, is secreted by various cells like macrophages, T cells, and adipocytes. It signals through TNFR1 and TNFR2 receptors, mediating immune responses, tumor cytotoxicity, and roles in septic shock, autoimmune diseases, and inflammation. TNF-β, produced by activated T and B cells, shares similar functions with TNF-α, including immune regulation, cell proliferation, apoptosis, and tumor cytotoxicity. Both TNF-α and TNF-β exhibit significant cross-reactivity between humans and mice, highlighting their conserved biological roles.

【Flow cytometry】A Review of Apoptosis Data Analysis Techniques

Post published:November 29, 2016
Post category:Apoptosis Technical Article
Post comments:0 Comments

Explore cutting-edge methods for analyzing apoptosis (programmed cell death) in biomedical research. Learn about flow cytometry, TUNEL assays, caspase detection, and computational modeling for accurate apoptosis measurement. Discover best practices for data interpretation in cancer, immunology, and drug development. 🧬 Read now for expert insights!

ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol

Post published:November 29, 2016
Post category:ELISA Technical Article
Post comments:0 Comments

Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.

Flow Cytometry Cell Cycle Analysis: PI Staining, Fixation vs. Live Cell Protocols & Key Considerations

Post published:November 29, 2016
Post category:Cell Cycle Staining Technical Article
Post comments:0 Comments

Learn the principles of cell cycle analysis by flow cytometry, including PI staining, RNase treatment, critical no-wash steps, and protocols for fixed & live cells. Includes step-by-step methods and troubleshooting tips for accurate results.

Interferons (IFNs): Types, Functions, and Their Role in Immunity & Disease

Post published:November 29, 2016
Post category:Target Tips
Post comments:0 Comments

Explore interferons (IFNs), key signaling proteins in immune defense against viruses, bacteria, and cancer. Learn about type I (IFN-α, IFN-β), type II (IFN-γ), and type III IFNs, their mechanisms, therapeutic uses, and links to autoimmune diseases. Discover research-grade IFN products for your studies.

ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects

Post published:November 29, 2016
Post category:ELISA Technical Article
Post comments:0 Comments

Learn whether to use serum or plasma for ELISA testing, including preparation protocols, key differences (fibrinogen, clotting factors), and how hemolysis impacts results. Essential guide for accurate immunoassays.