Human TNF-α High Sensitivity ELISA Kit

High Sensitivity ELISA

$380.00 – $480.00

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Datasheet

SDS File

TARGETS	TNF
SPECIES	Human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	Serum, plasma: 20 μL；cell culture supernatant: 100 μL
Sensitivity	0.16 pg/mL
Range	1.56 pg/mL – 100 pg/mL
Assay Time	3.5 h
Recovery	100% – 108%
Average Recovery	1.04

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	Serum, plasma: 20 μL；cell culture supernatant: 100 μL
Sensitivity	0.16 pg/mL
Range	1.56 pg/mL – 100 pg/mL
Assay Time	3.5 h
Recovery	100% – 108%
Average Recovery	1.04
Intra Precision	4.1% – 4.8%
Inter-Precision	5.4% – 7.0%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TNF-α monoclonal antibody Human TNF-α freeze-dried standard TNF-α detect Antibody Standard Diluent HRP-labeled streptavidin Signal enhancer concentrate Signal enhancer diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×)
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human TNF-α antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the TNF-α present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of TNF-α in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

TNF

TNF Target Infomation Overview

Target Symbol: TNF, tumor necrosis factor
Gene Groups: Tumor necrosis factor superfamily
Alias: TNFSF2; DIF; TNF-alpha
Previous Names: TNFA
Alias Names: TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2)

TNF, tumor necrosis factor Target Infomation by Species

HumanMouseRat

Human TNF Target Information

Target Symbol: TNF, tumor necrosis factor
Alias:
- APC1 protein
- cachectin
- DIF
- TNF superfamily, member 2
- TNF-a
- TNF-alpha
- TNF, macrophage-derived
- TNF, monocyte-derived
- TNFA
- TNFSF2
- TNLG1F
- tumor necrosis factor alpha
- tumor necrosis factor ligand 1F
- tumor necrosis factor ligand superfamily member 2
- tumor necrosis factor-alpha
NCBI_Gene: 7124
UniProtKB: P01375

Human TNF Predicted Functions

Enables several functions, including identical protein binding activity; protease binding activity; and signaling receptor binding activity. Involved in several processes, including negative regulation of macromolecule metabolic process; positive regulation of macromolecule metabolic process; and regulation of apoptotic process. Acts upstream of or within several processes, including regulation of cellular protein metabolic process; regulation of gene expression; and regulation of signal transduction. Located in cell surface; extracellular space; and membrane raft. Is integral component of plasma membrane. Implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Biomarker of several diseases, including anemia (multiple); autoimmune disease (multiple); eye disease (multiple); kidney disease (multiple); and lung disease (multiple).

Mouse Tnf Target Information

Target Symbol: Tnf, tumor necrosis factor
Alias:
- DIF
- TNF alpha
- TNF-alpha
- Tnfa
- TNFalpha
- Tnfsf1a
- tumor necrosis factor-alpha
- tumor necrosis factor, alpha
NCBI_Gene: 21926

Mouse Tnf Predicted Functions

Enables cytokine activity; protease binding activity; and tumor necrosis factor receptor binding activity. Involved in several processes, including endothelial cell apoptotic process; positive regulation of cell communication; and positive regulation of macromolecule metabolic process. Acts upstream of or within several processes, including apoptotic signaling pathway; positive regulation of intracellular signal transduction; and regulation of gene expression. Located in several cellular components, including external side of plasma membrane; phagocytic cup; and recycling endosome. Is expressed in several structures, including alimentary system; brain; integumental system; lung; and reproductive system. Used to study several diseases, including autoimmune disease (multiple); congestive heart failure; heart valve disease (multiple); idiopathic pulmonary fibrosis; and spondyloarthropathy. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Orthologous to human TNF (tumor necrosis factor).

Rat Tnf Target Information

Target Symbol: Tnf, tumor necrosis factor
Alias:
- cachectin
- LOC103694380
- MGC124630
- RATTNF
- TNF-a
- TNF-alpha
- Tnfa
- tumor necrosis factor (TNF superfamily, member 2)
- tumor necrosis factor alpha
- tumor necrosis factor ligand superfamily member 2
- tumor necrosis factor superfamily member 2
- tumor necrosis factor superfamily, member 2
- tumor necrosis factor-alpha
- tumor necrosis factor-like
NCBI_Gene: 24835
UniProtKB: P16599

Rat Tnf Predicted Functions

Enables tumor necrosis factor receptor binding activity. Involved in several processes, including positive regulation of cell communication; positive regulation of macromolecule metabolic process; and positive regulation of neuron death. Located in several cellular components, including external side of plasma membrane; extracellular space; and neuronal cell body. Used to study several diseases, including cerebrovascular disease (multiple); liver disease (multiple); peptic esophagitis; perinatal necrotizing enterocolitis; and periventricular leukomalacia. Biomarker of several diseases, including brain disease (multiple); congestive heart failure (multiple); inflammatory bowel disease (multiple); lung disease (multiple); and non-alcoholic fatty liver disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Orthologous to human TNF (tumor necrosis factor).