Human TNF-α High Sensitivity ELISA Kit

$380.00$480.00

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TARGETSTNF
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 20 μL;cell culture supernatant: 100 μL
Sensitivity0.16 pg/mL
Range1.56 pg/mL – 100 pg/mL
Assay Time3.5 h
Recovery100% – 108%
Average Recovery1.04
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 20 μL;cell culture supernatant: 100 μL
Sensitivity0.16 pg/mL
Range1.56 pg/mL – 100 pg/mL
Assay Time3.5 h
Recovery100% – 108%
Average Recovery1.04
Intra Precision4.1% – 4.8%
Inter-Precision5.4% – 7.0%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TNF-α monoclonal antibody
Human TNF-α freeze-dried standard
TNF-α detect Antibody
Standard Diluent
HRP-labeled streptavidin
Signal enhancer concentrate
Signal enhancer diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human TNF-α antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the TNF-α present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of TNF-α in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

TNF

TNF Target Infomation Overview

  • Target Symbol: TNF, tumor necrosis factor
  • Gene Groups: Tumor necrosis factor superfamily
  • Alias: TNFSF2; DIF; TNF-alpha
  • Previous Names: TNFA
  • Alias Names: TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2)

TNF, tumor necrosis factor Target Infomation by Species

HumanMouseRat

Human TNF Target Information

  • Target Symbol: TNF, tumor necrosis factor
  • Alias:
    • APC1 protein
    • cachectin
    • DIF
    • TNF superfamily, member 2
    • TNF-a
    • TNF-alpha
    • TNF, macrophage-derived
    • TNF, monocyte-derived
    • TNFA
    • TNFSF2
    • TNLG1F
    • tumor necrosis factor alpha
    • tumor necrosis factor ligand 1F
    • tumor necrosis factor ligand superfamily member 2
    • tumor necrosis factor-alpha
  • NCBI_Gene: 7124
  • UniProtKB: P01375

Human TNF Predicted Functions

Enables several functions, including identical protein binding activity; protease binding activity; and signaling receptor binding activity. Involved in several processes, including negative regulation of macromolecule metabolic process; positive regulation of macromolecule metabolic process; and regulation of apoptotic process. Acts upstream of or within several processes, including regulation of cellular protein metabolic process; regulation of gene expression; and regulation of signal transduction. Located in cell surface; extracellular space; and membrane raft. Is integral component of plasma membrane. Implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Biomarker of several diseases, including anemia (multiple); autoimmune disease (multiple); eye disease (multiple); kidney disease (multiple); and lung disease (multiple).

Mouse Tnf Target Information

Mouse Tnf Predicted Functions

Enables cytokine activity; protease binding activity; and tumor necrosis factor receptor binding activity. Involved in several processes, including endothelial cell apoptotic process; positive regulation of cell communication; and positive regulation of macromolecule metabolic process. Acts upstream of or within several processes, including apoptotic signaling pathway; positive regulation of intracellular signal transduction; and regulation of gene expression. Located in several cellular components, including external side of plasma membrane; phagocytic cup; and recycling endosome. Is expressed in several structures, including alimentary system; brain; integumental system; lung; and reproductive system. Used to study several diseases, including autoimmune disease (multiple); congestive heart failure; heart valve disease (multiple); idiopathic pulmonary fibrosis; and spondyloarthropathy. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Orthologous to human TNF (tumor necrosis factor).

Rat Tnf Target Information

  • Target Symbol: Tnf, tumor necrosis factor
  • Alias:
    • cachectin
    • LOC103694380
    • MGC124630
    • RATTNF
    • TNF-a
    • TNF-alpha
    • Tnfa
    • tumor necrosis factor (TNF superfamily, member 2)
    • tumor necrosis factor alpha
    • tumor necrosis factor ligand superfamily member 2
    • tumor necrosis factor superfamily member 2
    • tumor necrosis factor superfamily, member 2
    • tumor necrosis factor-alpha
    • tumor necrosis factor-like
  • NCBI_Gene: 24835
  • UniProtKB: P16599

Rat Tnf Predicted Functions

Enables tumor necrosis factor receptor binding activity. Involved in several processes, including positive regulation of cell communication; positive regulation of macromolecule metabolic process; and positive regulation of neuron death. Located in several cellular components, including external side of plasma membrane; extracellular space; and neuronal cell body. Used to study several diseases, including cerebrovascular disease (multiple); liver disease (multiple); peptic esophagitis; perinatal necrotizing enterocolitis; and periventricular leukomalacia. Biomarker of several diseases, including brain disease (multiple); congestive heart failure (multiple); inflammatory bowel disease (multiple); lung disease (multiple); and non-alcoholic fatty liver disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Orthologous to human TNF (tumor necrosis factor).

TNF Target News

    4.8
    OTUB1’s role in promoting OSCC development by stabilizing RACK1 involves cell proliferation, migration, invasion, and tumor-associated macrophage M1 polarization
    CELLULAR SIGNALLING
    5.9
    Selenium-Enriched Soybean Peptides as Novel Organic Selenium Compound Supplements: Inhibition of Occupational Air Pollution Exposure-Induced Apoptosis in Lung Epithelial Cells
    Nutrients
    4.6
    Dendrobium officinale polysaccharide Converts M2 into M1 Subtype Macrophage Polarization via the STAT6/PPAR-r and JAGGED1/NOTCH1 Signaling Pathways to Inhibit Gastric Cancer
    MOLECULES
    4.927
    Confusoside from Anneslea fragrans Alleviates Acetaminophen-Induced Liver Injury in HepG2 via PI3K-CASP3 Signaling Pathway
    MOLECULES
    5.561
    Ultra-High Hydrostatic Pressure Pretreatment on White Que Zui Tea: Chemical Constituents, Antioxidant, Cytoprotective, and Anti-Inflammatory Activities
    Foods
    2.7
    TIPE2 regulates periodontal inflammation by inhibiting NF-κB p65 phosphorylation
    Journal of Applied Oral Science
    11.3
    Fe-curcumin nanozyme-mediated immunosuppression and anti-inflammation in experimental autoimmune uveitis
    Biomaterials Research
    10.9
    Targeting and cytotoxicity of chimeric antigen receptor T cells grafted with PD1 extramembrane domain
    Experimental Hematology & Oncology
    8.593
    Hypoxia-associated circPRDM4 promotes immune escape via HIF-1α regulation of PD-L1 in hepatocellular carcinoma
    Experimental Hematology & Oncology
    3.2
    Echinococcus granulosus cyst fluid inhibits inflammatory responses through inducing histone demethylase KDM5B in macrophages
    Parasites & Vectors
    4.546
    Gegen Qinlian standard decoction alleviated irinotecan-induced diarrhea via PI3K/AKT/NF-κB axis by network pharmacology prediction and experimental validation combination
    Chinese Medicine
    6.376
    4-Octyl itaconate attenuates glycemic deterioration by regulating macrophage polarization in mouse models of type 1 diabetes
    MOLECULAR MEDICINE
    3.654
    Kaempferol Reverses Acute Kidney Injury in Septic Model by Inhibiting NF-κB/AKT Signaling Pathway
    JOURNAL OF FOOD BIOCHEMISTRY
    0.8
    Impact of intravenous administration of nalbuphine at different time points for postoperative analgesia and sedation in adenotonsillectomized children: a prospective, randomized controlled trial
    World Journal of Pediatric Surgery
    2.5
    Protective effect of small extracellular vesicles (EVs) derived from ACE2-modified human umbilical cord mesenchymal stem cells against renal ischemia–reperfusion injury
    NEPHROLOGY
    2.189
    Maslinic acid attenuates UVB-induced oxidative damage in HFF-1 cells
    Journal of Cosmetic Dermatology
    Allergic Reaction
    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Eye Diseases (Glaucoma & Cataract)
    Lung Disease (Asthma & COPD)
    Multiple Sclerosis
    Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
    Obesity Diabetes
    Osteoarthrosis & Rheumatoid Arthritis
    Osteoporosis
    Sepsis
    Systemic Lupus Erythematosus
    Transplantation
    Tumor Marker

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