EasyGo!™ Human TNF-α One-Step ELISA Kit

EK182EG

EasyGo!™ Human TNF-α One-Step ELISA Kit

$349.00 – $499.00

 DATASHEET
Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20uL
Sensitivity5.40pg/ml
Array Range15.63-1000pg/ml
Assay Time90min
Recovery70%-130%
Average Recovery/
SKU: EK182EG Category:

ELISA Kit Detail Information

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20uL
Sensitivity5.40pg/ml
Array Range15.63-1000pg/ml
Assay Time90min
Recovery70%-130%
Average Recovery/
Intra Precision< 10%
Inter Precision< 10%
PlateDetachable 96-well Plate
Storage2-8℃
Deliveryblue ice
ComponentsCoated Microplate
standard
Assay Buffer (10×)
TMB
Stop Solution
Washing Buffer (20×)
Assay PrincipleThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Mouse IL-18 has been immobilized onto microwells, and one pellet of the HRP-linked detect antibody specific for IL-18 (light yellow) is pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IL-18 present is bound by the immobilized antibody and detect antibody in the incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-18 bound by the immobilized antibody. The color development is stopped and the intensity of the color is measured by microplate reader.

Related Targets

TNF

TNF Target Infomation Overview

  • Target Symbol: TNF, tumor necrosis factor
  • Gene Groups: Tumor necrosis factor superfamily
  • Alias: TNFSF2; DIF; TNF-alpha
  • Previous Names: TNFA
  • Alias Names: TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2)

TNF, tumor necrosis factor Target Infomation by Species

  • Human
  • Mouse
  • Rat

Human TNF Target Information

  • Target Symbol: TNF, tumor necrosis factor
  • Alias:
    • APC1 protein
    • cachectin
    • DIF
    • TNF superfamily, member 2
    • TNF-a
    • TNF-alpha
    • TNF, macrophage-derived
    • TNF, monocyte-derived
    • TNFA
    • TNFSF2
    • TNLG1F
    • tumor necrosis factor alpha
    • tumor necrosis factor ligand 1F
    • tumor necrosis factor ligand superfamily member 2
    • tumor necrosis factor-alpha
  • NCBI_Gene: 7124
  • UniProtKB: P01375

Human TNF Predicted Functions

Enables several functions, including identical protein binding activity; protease binding activity; and signaling receptor binding activity. Involved in several processes, including negative regulation of macromolecule metabolic process; positive regulation of macromolecule metabolic process; and regulation of apoptotic process. Acts upstream of or within several processes, including regulation of cellular protein metabolic process; regulation of gene expression; and regulation of signal transduction. Located in cell surface; extracellular space; and membrane raft. Is integral component of plasma membrane. Implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Biomarker of several diseases, including anemia (multiple); autoimmune disease (multiple); eye disease (multiple); kidney disease (multiple); and lung disease (multiple).

Mouse Tnf Target Information

Mouse Tnf Predicted Functions

Enables cytokine activity; protease binding activity; and tumor necrosis factor receptor binding activity. Involved in several processes, including endothelial cell apoptotic process; positive regulation of cell communication; and positive regulation of macromolecule metabolic process. Acts upstream of or within several processes, including apoptotic signaling pathway; positive regulation of intracellular signal transduction; and regulation of gene expression. Located in several cellular components, including external side of plasma membrane; phagocytic cup; and recycling endosome. Is expressed in several structures, including alimentary system; brain; integumental system; lung; and reproductive system. Used to study several diseases, including autoimmune disease (multiple); congestive heart failure; heart valve disease (multiple); idiopathic pulmonary fibrosis; and spondyloarthropathy. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Orthologous to human TNF (tumor necrosis factor).

Rat Tnf Target Information

  • Target Symbol: Tnf, tumor necrosis factor
  • Alias:
    • cachectin
    • LOC103694380
    • MGC124630
    • RATTNF
    • TNF-a
    • TNF-alpha
    • Tnfa
    • tumor necrosis factor (TNF superfamily, member 2)
    • tumor necrosis factor alpha
    • tumor necrosis factor ligand superfamily member 2
    • tumor necrosis factor superfamily member 2
    • tumor necrosis factor superfamily, member 2
    • tumor necrosis factor-alpha
    • tumor necrosis factor-like
  • NCBI_Gene: 24835
  • UniProtKB: P16599

Rat Tnf Predicted Functions

Enables tumor necrosis factor receptor binding activity. Involved in several processes, including positive regulation of cell communication; positive regulation of macromolecule metabolic process; and positive regulation of neuron death. Located in several cellular components, including external side of plasma membrane; extracellular space; and neuronal cell body. Used to study several diseases, including cerebrovascular disease (multiple); liver disease (multiple); peptic esophagitis; perinatal necrotizing enterocolitis; and periventricular leukomalacia. Biomarker of several diseases, including brain disease (multiple); congestive heart failure (multiple); inflammatory bowel disease (multiple); lung disease (multiple); and non-alcoholic fatty liver disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Orthologous to human TNF (tumor necrosis factor).

Products with the Same Target - TNF

EasyGo!™ Mouse TNF-α One-Step ELISA Kit

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Rat TNF-α High Sensitivity ELISA Kit

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Rat TNF-a ELISA Kit

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Mouse TNF-α High Sensitivity ELISA Kit

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Mouse TNF-a ELISA Kit

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Human TNF-α High Sensitivity ELISA Kit

$319.00 – $449.00
Competitive ELISA: Principles, Methods, and Key Differences

Competitive ELISA: Principles, Methods, and Key Differences

Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.

Continue Reading Competitive ELISA: Principles, Methods, and Key Differences

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